Paracoccus sulfuroxidans
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3917 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A562NMA5|A0A562NMA5_9RHOB 16S rRNA (Guanine1207-N2)-methyltransferase OS=Paracoccus sulfuroxidans OX=384678 GN=IQ24_02755 PE=4 SV=1
MM1 pKa = 7.01 TVSRR5 pKa = 11.84 LNTGFTTTGAISYY18 pKa = 9.87 LGEE21 pKa = 4.12 SDD23 pKa = 3.86 TYY25 pKa = 10.6 NTTFIQGLTYY35 pKa = 9.95 SVRR38 pKa = 11.84 VSGASSGGGSLADD51 pKa = 4.28 PNLGLYY57 pKa = 7.59 NTAGQRR63 pKa = 11.84 LLFNDD68 pKa = 5.33 DD69 pKa = 3.28 INPGVNRR76 pKa = 11.84 DD77 pKa = 3.34 SALTFTVNQTSDD89 pKa = 2.96 YY90 pKa = 10.4 RR91 pKa = 11.84 MLVGEE96 pKa = 4.4 QGNNATGSYY105 pKa = 9.42 TIAISAGYY113 pKa = 10.33 ANNNNNTVYY122 pKa = 9.36 GTYY125 pKa = 10.51 NADD128 pKa = 3.36 AVNGMGGNDD137 pKa = 3.66 ILNGGAGNDD146 pKa = 3.92 RR147 pKa = 11.84 LWGGTGNDD155 pKa = 3.25 QLLGGLHH162 pKa = 7.03 NDD164 pKa = 4.16 TLWGEE169 pKa = 4.08 QGNDD173 pKa = 3.17 VLRR176 pKa = 11.84 GGEE179 pKa = 4.25 GNDD182 pKa = 3.53 ALVGGAGADD191 pKa = 3.57 RR192 pKa = 11.84 LIGGNGADD200 pKa = 2.37 RR201 pKa = 11.84 FVFNFASDD209 pKa = 3.9 SARR212 pKa = 11.84 GAPDD216 pKa = 4.42 TIAMGDD222 pKa = 3.72 GAIAMQGVGVAGGDD236 pKa = 3.78 VIDD239 pKa = 5.8 LSGIDD244 pKa = 3.93 ANVNYY249 pKa = 10.38 AGNQAFTWSTSHH261 pKa = 7.47 AAGTLSLSEE270 pKa = 4.28 QNGSTVVQGHH280 pKa = 5.45 VNNDD284 pKa = 3.95 GIADD288 pKa = 4.58 FVLVIADD295 pKa = 3.7 GAGINAFSYY304 pKa = 9.96 TSNEE308 pKa = 3.84 FVLL311 pKa = 4.62
Molecular weight: 31.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.834
IPC_protein 3.859
Toseland 3.617
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.681
Grimsley 3.528
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.291
Thurlkill 3.681
EMBOSS 3.846
Sillero 3.986
Patrickios 1.926
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|A0A562NX08|A0A562NX08_9RHOB Microcin C transport system permease protein OS=Paracoccus sulfuroxidans OX=384678 GN=IQ24_00526 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 ARR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.37 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.12 GGRR29 pKa = 11.84 RR30 pKa = 11.84 VLNARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.0 GRR40 pKa = 11.84 KK41 pKa = 8.91 SLSAA45 pKa = 3.86
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3917
0
3917
1217529
39
2200
310.8
33.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.458 ± 0.049
0.785 ± 0.013
5.888 ± 0.037
5.728 ± 0.037
3.527 ± 0.023
8.82 ± 0.051
1.995 ± 0.019
5.265 ± 0.024
2.909 ± 0.034
10.206 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.792 ± 0.018
2.643 ± 0.03
5.37 ± 0.033
3.528 ± 0.025
7.044 ± 0.043
5.203 ± 0.027
5.326 ± 0.031
6.992 ± 0.031
1.429 ± 0.019
2.092 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here