Kwoniella shandongensis
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7422 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5M6BS84|A0A5M6BS84_9TREE Uncharacterized protein OS=Kwoniella shandongensis OX=1734106 GN=CI109_007192 PE=4 SV=1
MM1 pKa = 7.3 FSKK4 pKa = 10.59 FALVTLTAIIGAGVGVTAAPFTKK27 pKa = 9.8 RR28 pKa = 11.84 DD29 pKa = 3.94 TNVLLYY35 pKa = 10.03 SQKK38 pKa = 11.39 DD39 pKa = 3.71 NLCLSVEE46 pKa = 4.42 TGAQANDD53 pKa = 3.14 QTQAYY58 pKa = 8.87 SNHH61 pKa = 5.97 SSPYY65 pKa = 8.43 LYY67 pKa = 10.36 EE68 pKa = 4.03 GLRR71 pKa = 11.84 VVSLPCEE78 pKa = 4.24 NSSTWDD84 pKa = 3.37 VSQGNGSVYY93 pKa = 9.13 VTNSDD98 pKa = 3.24 QAFALEE104 pKa = 4.67 AGPHH108 pKa = 5.49 VDD110 pKa = 3.59 NFQTLWVNKK119 pKa = 8.22 TYY121 pKa = 10.06 PGKK124 pKa = 10.06 YY125 pKa = 9.04 SQTFFYY131 pKa = 10.57 TGDD134 pKa = 3.23 RR135 pKa = 11.84 RR136 pKa = 11.84 LAVTNSTEE144 pKa = 4.55 CVDD147 pKa = 3.9 EE148 pKa = 4.63 GDD150 pKa = 4.42 NGAQEE155 pKa = 4.38 YY156 pKa = 10.71 SCTTGDD162 pKa = 3.89 TNQIWDD168 pKa = 3.76 ATSPNGPLDD177 pKa = 4.18 PQPLTTFASSVSTAAATASPASASSDD203 pKa = 3.45 VTSASSGDD211 pKa = 3.91 DD212 pKa = 3.49 PSTATADD219 pKa = 3.66 ATSVTAAGATASTGSSPTDD238 pKa = 3.38 NASGSSDD245 pKa = 2.63 IAAAATTSPSDD256 pKa = 3.38 TASAATASADD266 pKa = 3.27 ITRR269 pKa = 11.84 LASSPSSDD277 pKa = 3.05 VAAAATASPAFTAATFALGGEE298 pKa = 4.71 PEE300 pKa = 4.65 SSTAPASAASDD311 pKa = 3.61 SASASSCKK319 pKa = 10.02 RR320 pKa = 11.84 SGANN324 pKa = 3.14
Molecular weight: 32.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.923
IPC_protein 3.935
Toseland 3.706
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.884
Rodwell 3.757
Grimsley 3.617
Solomon 3.923
Lehninger 3.884
Nozaki 4.05
DTASelect 4.317
Thurlkill 3.77
EMBOSS 3.897
Sillero 4.062
Patrickios 1.163
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.923
Protein with the highest isoelectric point:
>tr|A0A5M6BWC3|A0A5M6BWC3_9TREE L51_S25_CI-B8 domain-containing protein OS=Kwoniella shandongensis OX=1734106 GN=CI109_004470 PE=4 SV=1
MM1 pKa = 7.5 PRR3 pKa = 11.84 LPRR6 pKa = 11.84 TAFLRR11 pKa = 11.84 SLPLPPLQPRR21 pKa = 11.84 LALPSVSRR29 pKa = 11.84 TTLTPSTFTSSFASLRR45 pKa = 11.84 LSPLNSSLITSSLRR59 pKa = 11.84 PSISNLPTHH68 pKa = 6.83 ILSSSSTSQIQLGGVRR84 pKa = 11.84 GAAMGTFYY92 pKa = 10.92 QPSQRR97 pKa = 11.84 KK98 pKa = 8.72 RR99 pKa = 11.84 KK100 pKa = 8.95 NKK102 pKa = 9.88 HH103 pKa = 4.66 GFLARR108 pKa = 11.84 LRR110 pKa = 11.84 GGKK113 pKa = 7.91 NARR116 pKa = 11.84 KK117 pKa = 8.1 MLSRR121 pKa = 11.84 RR122 pKa = 11.84 LLKK125 pKa = 10.3 GRR127 pKa = 11.84 KK128 pKa = 8.56 NLSHH132 pKa = 7.01
Molecular weight: 14.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.497
IPC2_protein 11.272
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.515
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.237
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.133
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7422
0
7422
3956821
40
4986
533.1
58.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.478 ± 0.026
0.911 ± 0.008
5.541 ± 0.021
6.405 ± 0.026
3.265 ± 0.017
7.519 ± 0.027
2.31 ± 0.013
4.609 ± 0.018
4.918 ± 0.024
8.724 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.043 ± 0.011
3.293 ± 0.013
6.793 ± 0.032
3.73 ± 0.023
6.026 ± 0.023
9.247 ± 0.041
6.299 ± 0.02
6.083 ± 0.019
1.351 ± 0.009
2.456 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here