Desulfotomaculum nigrificans (strain DSM 14880 / VKM B-2319 / CO-1-SRB) (Desulfotomaculum carboxydivorans)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfotomaculum; Desulfotomaculum nigrificans

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2620 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6B4I8|F6B4I8_DESCC Spore cortex biosynthesis protein YabQ OS=Desulfotomaculum nigrificans (strain DSM 14880 / VKM B-2319 / CO-1-SRB) OX=868595 GN=Desca_0106 PE=4 SV=1
MM1 pKa = 7.52SNQEE5 pKa = 3.73EE6 pKa = 4.63GRR8 pKa = 11.84PDD10 pKa = 3.35MLEE13 pKa = 3.93TGVSSWSYY21 pKa = 10.32SPEE24 pKa = 4.12VPDD27 pKa = 4.35EE28 pKa = 4.03EE29 pKa = 4.73MNVDD33 pKa = 5.74FYY35 pKa = 11.54YY36 pKa = 10.79NWSAHH41 pKa = 4.32WW42 pKa = 3.71

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6B7E6|F6B7E6_DESCC Transposase IS605 OrfB family OS=Desulfotomaculum nigrificans (strain DSM 14880 / VKM B-2319 / CO-1-SRB) OX=868595 GN=Desca_0504 PE=4 SV=1
MM1 pKa = 7.36NLEE4 pKa = 4.11TNLANSRR11 pKa = 11.84SSSHH15 pKa = 4.93SRR17 pKa = 11.84AWLLILGIILLASTLRR33 pKa = 11.84APLTAVGPLIGFIRR47 pKa = 11.84DD48 pKa = 3.37DD49 pKa = 3.65TGISNTFAGLLTTFPLLAFAFFSPLAPKK77 pKa = 10.13LARR80 pKa = 11.84RR81 pKa = 11.84FGTEE85 pKa = 3.48YY86 pKa = 10.68TLFASLIVMSLGIVLRR102 pKa = 11.84SLRR105 pKa = 11.84SLEE108 pKa = 3.97TLLVGTALIGIAIAFGNVLLPSLIKK133 pKa = 9.96RR134 pKa = 11.84DD135 pKa = 3.59FPEE138 pKa = 4.13RR139 pKa = 11.84VGLMTGIYY147 pKa = 9.85SIFMNMWAAVASGISIPLAQKK168 pKa = 10.73LGFGWRR174 pKa = 11.84GSLICWSVLSIVSLVAWLPQLRR196 pKa = 11.84VCQRR200 pKa = 11.84PSTSQGVQTPNNNLWHH216 pKa = 6.83SRR218 pKa = 11.84LAWQVSVFMGLQSLTFYY235 pKa = 11.73VMIAWLPEE243 pKa = 3.53ILHH246 pKa = 5.26QQGINHH252 pKa = 6.41SLAGWMLSLMQFVSLPTTLIIPVLAGRR279 pKa = 11.84CSNQRR284 pKa = 11.84RR285 pKa = 11.84LVGFGAVILLTGYY298 pKa = 10.14IGLLSRR304 pKa = 11.84NTLLAPLWIVLIGIAVGTNFSLALIFFVLRR334 pKa = 11.84TQNANDD340 pKa = 3.67AAEE343 pKa = 4.3LSGMAQSIGYY353 pKa = 8.53FLAAVGPPLLGFVHH367 pKa = 7.56DD368 pKa = 4.57MTQSWTAPLCILVVIAILLFIFGLAAGSKK397 pKa = 10.75GYY399 pKa = 8.15VTSKK403 pKa = 11.23NNN405 pKa = 3.12

Molecular weight: 43.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2620

0

2620

789997

30

2306

301.5

33.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.523 ± 0.05

1.199 ± 0.022

4.826 ± 0.037

6.508 ± 0.053

3.667 ± 0.032

7.781 ± 0.041

1.889 ± 0.018

6.982 ± 0.041

6.074 ± 0.048

10.34 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.619 ± 0.022

3.971 ± 0.03

4.363 ± 0.033

4.004 ± 0.034

5.223 ± 0.045

4.936 ± 0.031

5.171 ± 0.032

7.853 ± 0.036

0.964 ± 0.018

3.106 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski