Candidatus Accumulibacter sp. SK-11

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Accumulibacter; unclassified Candidatus Accumulibacter

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4767 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A011NK49|A0A011NK49_9PROT Arsenate reductase OS=Candidatus Accumulibacter sp. SK-11 OX=1454000 GN=arsC PE=3 SV=1
MM1 pKa = 7.78ASFSPNMPTTANGRR15 pKa = 11.84TVTSQGVYY23 pKa = 10.28SDD25 pKa = 4.81PLLPSYY31 pKa = 9.91WYY33 pKa = 10.92LGDD36 pKa = 3.56ASGAVKK42 pKa = 10.28GVNAMRR48 pKa = 11.84AWDD51 pKa = 4.24DD52 pKa = 3.49YY53 pKa = 10.08RR54 pKa = 11.84GSGIVVAVIDD64 pKa = 4.51DD65 pKa = 4.03GVEE68 pKa = 4.03YY69 pKa = 9.66THH71 pKa = 7.43LDD73 pKa = 2.93LAANYY78 pKa = 10.01RR79 pKa = 11.84SDD81 pKa = 3.38LAYY84 pKa = 8.8DD85 pKa = 3.53TRR87 pKa = 11.84DD88 pKa = 3.21RR89 pKa = 11.84DD90 pKa = 3.33ADD92 pKa = 3.75AFPGEE97 pKa = 4.17SSDD100 pKa = 3.5RR101 pKa = 11.84HH102 pKa = 4.55GTAVSGVIAAALNNGVGGAGVAPGASLVGYY132 pKa = 10.17RR133 pKa = 11.84IGFGANGTLEE143 pKa = 4.01QLVAAFQLLTAVDD156 pKa = 3.8VANNSWGFDD165 pKa = 3.23GFFGDD170 pKa = 5.32NFLDD174 pKa = 3.92PDD176 pKa = 4.39FAPIGDD182 pKa = 4.29ALATALAAGRR192 pKa = 11.84GGLGTIVVMAAGNARR207 pKa = 11.84TSGQDD212 pKa = 3.2VNYY215 pKa = 10.56HH216 pKa = 6.04GFQNHH221 pKa = 6.65RR222 pKa = 11.84GTIAVAATDD231 pKa = 3.12SGGNVTYY238 pKa = 10.87YY239 pKa = 9.08STPGAALLVAAPGHH253 pKa = 6.59GITTTDD259 pKa = 3.13RR260 pKa = 11.84VDD262 pKa = 3.29GAGYY266 pKa = 11.0ASGDD270 pKa = 3.7YY271 pKa = 9.25ATLNGTSFAAPMVSGIAALLLDD293 pKa = 4.93ANPGLGWRR301 pKa = 11.84DD302 pKa = 3.42VQEE305 pKa = 4.15ILAATAVRR313 pKa = 11.84TGSPASWSFNAADD326 pKa = 3.44NWNGGGMHH334 pKa = 6.65VSHH337 pKa = 8.04DD338 pKa = 3.9YY339 pKa = 11.64GFGLVDD345 pKa = 4.02AYY347 pKa = 10.94AAVRR351 pKa = 11.84VAEE354 pKa = 4.31SWRR357 pKa = 11.84SVSTSLNEE365 pKa = 3.74WVAEE369 pKa = 4.02GLQYY373 pKa = 10.18PASPIAIPDD382 pKa = 3.85GGSASSTITLAAGLRR397 pKa = 11.84IDD399 pKa = 4.02RR400 pKa = 11.84VEE402 pKa = 3.78VDD404 pKa = 3.76LALAHH409 pKa = 6.93PYY411 pKa = 9.86LVQLRR416 pKa = 11.84VTLTAPDD423 pKa = 3.66GTEE426 pKa = 3.77SVLVQNPSTSQGNIYY441 pKa = 8.94FTFSTTRR448 pKa = 11.84DD449 pKa = 2.93WGEE452 pKa = 3.61FSGGNWTLTVTDD464 pKa = 4.05MQVGATGVVYY474 pKa = 10.27AWGIRR479 pKa = 11.84AYY481 pKa = 10.67GDD483 pKa = 3.39LAGDD487 pKa = 3.54DD488 pKa = 3.9TYY490 pKa = 11.74LYY492 pKa = 9.57TGEE495 pKa = 4.12FAALSAADD503 pKa = 3.32ASRR506 pKa = 11.84RR507 pKa = 11.84VLSDD511 pKa = 3.02AGGMDD516 pKa = 5.56AINAAAIAGDD526 pKa = 3.79TLLDD530 pKa = 3.6LRR532 pKa = 11.84PAHH535 pKa = 6.39VSLIAGQEE543 pKa = 4.0VTISAGTIIEE553 pKa = 4.27NADD556 pKa = 3.56SGDD559 pKa = 3.96GNDD562 pKa = 3.72TLIGNDD568 pKa = 3.33AANSLRR574 pKa = 11.84GWRR577 pKa = 11.84GNDD580 pKa = 3.35FLDD583 pKa = 4.29GGAGVDD589 pKa = 4.03TLDD592 pKa = 4.07GGAGVDD598 pKa = 3.76TLDD601 pKa = 4.23GGVGDD606 pKa = 4.55DD607 pKa = 3.9VYY609 pKa = 11.62VVDD612 pKa = 4.21VAADD616 pKa = 3.82VIVEE620 pKa = 4.16RR621 pKa = 11.84PGGGTDD627 pKa = 3.39TVRR630 pKa = 11.84TTLASYY636 pKa = 11.29LLGLEE641 pKa = 4.3LEE643 pKa = 4.34NLVFVGSGNFKK654 pKa = 9.67GTGNAAANVIDD665 pKa = 4.68GGAGNDD671 pKa = 3.8SLNGGLGADD680 pKa = 4.6LLRR683 pKa = 11.84GGLGDD688 pKa = 3.64DD689 pKa = 4.39TYY691 pKa = 11.24TVDD694 pKa = 3.14HH695 pKa = 7.0AGDD698 pKa = 3.98SVVEE702 pKa = 4.36LPGEE706 pKa = 4.39GNDD709 pKa = 3.64YY710 pKa = 10.98VYY712 pKa = 11.37SSVSWTLGANLEE724 pKa = 4.06RR725 pKa = 11.84LYY727 pKa = 10.39LTGSAAIDD735 pKa = 3.75GAGNDD740 pKa = 4.03LGNRR744 pKa = 11.84LYY746 pKa = 10.83GQSNSAINTLAGGPGNDD763 pKa = 3.31TYY765 pKa = 11.87YY766 pKa = 10.99VGSKK770 pKa = 10.7DD771 pKa = 4.43VIVEE775 pKa = 4.02LAGEE779 pKa = 4.52GTDD782 pKa = 3.25TAYY785 pKa = 10.49GYY787 pKa = 11.65GDD789 pKa = 3.56YY790 pKa = 10.46TLAAGVSVEE799 pKa = 3.78YY800 pKa = 9.77FYY802 pKa = 11.2INVTTGHH809 pKa = 6.03TLAGNEE815 pKa = 4.18LANNLRR821 pKa = 11.84GNSGNDD827 pKa = 3.21TLIGFEE833 pKa = 5.11GNDD836 pKa = 3.7SLNGGLGVDD845 pKa = 4.7LLRR848 pKa = 11.84GGPGDD853 pKa = 3.55DD854 pKa = 4.06TYY856 pKa = 11.5TVDD859 pKa = 3.14HH860 pKa = 7.0AGDD863 pKa = 3.9SVVEE867 pKa = 3.85LLGEE871 pKa = 4.51GKK873 pKa = 8.7DD874 pKa = 3.65TVYY877 pKa = 11.28SSVSWTLGDD886 pKa = 3.55HH887 pKa = 7.32LEE889 pKa = 4.03RR890 pKa = 11.84LYY892 pKa = 10.68LTGNAAIAGAGNEE905 pKa = 4.2LANTLVGYY913 pKa = 8.77TNAAGNALAGGAGDD927 pKa = 3.6DD928 pKa = 4.47AYY930 pKa = 11.5YY931 pKa = 11.16VDD933 pKa = 4.62ANDD936 pKa = 3.58VVVEE940 pKa = 4.04LVGEE944 pKa = 4.48GNDD947 pKa = 3.04IVYY950 pKa = 10.67GSVSWTLGANLEE962 pKa = 4.06RR963 pKa = 11.84LYY965 pKa = 10.39LTGSAAIDD973 pKa = 3.63GTGNDD978 pKa = 4.24LDD980 pKa = 3.72NRR982 pKa = 11.84LYY984 pKa = 10.93GQANGAINTLTGGTGNDD1001 pKa = 2.95IYY1003 pKa = 11.52YY1004 pKa = 10.53VGSNDD1009 pKa = 3.94VIVEE1013 pKa = 4.01LAVEE1017 pKa = 4.53GTDD1020 pKa = 3.16TAYY1023 pKa = 10.48GYY1025 pKa = 11.59GDD1027 pKa = 3.52YY1028 pKa = 10.66TLATGVSVEE1037 pKa = 3.9NLYY1040 pKa = 11.42LNVTTGQTLTGNEE1053 pKa = 4.32LANKK1057 pKa = 10.19LSGNAGSDD1065 pKa = 3.42TLRR1068 pKa = 11.84GLDD1071 pKa = 4.14GNDD1074 pKa = 3.46SLSGGLGADD1083 pKa = 3.76VLDD1086 pKa = 4.66GGQGNDD1092 pKa = 3.41TLAGGLGNDD1101 pKa = 3.83TVTGGNGNDD1110 pKa = 2.94IFRR1113 pKa = 11.84FATALDD1119 pKa = 4.02ANSNLDD1125 pKa = 3.43SVIDD1129 pKa = 3.82FNVVDD1134 pKa = 6.44DD1135 pKa = 5.08SFQLEE1140 pKa = 4.13NGIFTSLTQTGTLAVGLFVIGTAALDD1166 pKa = 4.08ANDD1169 pKa = 4.0KK1170 pKa = 10.85LIYY1173 pKa = 10.68DD1174 pKa = 4.02NTTGALFYY1182 pKa = 10.91DD1183 pKa = 4.5LDD1185 pKa = 4.36GSGSGGAIQFAVLSTNLALTNLDD1208 pKa = 3.86FVVTT1212 pKa = 4.39

Molecular weight: 123.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A011P3T9|A0A011P3T9_9PROT OsmC-like protein OS=Candidatus Accumulibacter sp. SK-11 OX=1454000 GN=AW07_03572 PE=4 SV=1
MM1 pKa = 7.37SAITRR6 pKa = 11.84MSVFGSLAAVSIIRR20 pKa = 11.84SAHH23 pKa = 4.39IVARR27 pKa = 11.84FRR29 pKa = 11.84SALPAAIRR37 pKa = 11.84RR38 pKa = 11.84PTRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84FSMTASRR50 pKa = 11.84SMIGIAHH57 pKa = 6.9SSPSFSAVTVV67 pKa = 3.6

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4767

0

4767

1187205

29

4072

249.0

27.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.706 ± 0.059

1.247 ± 0.015

5.359 ± 0.033

5.459 ± 0.04

3.319 ± 0.022

8.241 ± 0.044

2.329 ± 0.018

4.325 ± 0.026

2.526 ± 0.035

10.995 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.15 ± 0.017

2.297 ± 0.024

5.296 ± 0.033

3.977 ± 0.027

8.624 ± 0.047

5.718 ± 0.039

4.582 ± 0.028

7.392 ± 0.046

1.341 ± 0.016

1.967 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski