Amino acid dipepetide frequency for Pseudomonas phage vB_PaeS_PAO1_Ab18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.385AlaAla: 10.385 ± 0.963
0.959AlaCys: 0.959 ± 0.276
4.854AlaAsp: 4.854 ± 0.572
8.24AlaGlu: 8.24 ± 0.868
3.386AlaPhe: 3.386 ± 0.456
6.886AlaGly: 6.886 ± 0.66
2.032AlaHis: 2.032 ± 0.329
3.725AlaIle: 3.725 ± 0.458
5.136AlaLys: 5.136 ± 0.528
9.143AlaLeu: 9.143 ± 0.787
1.806AlaMet: 1.806 ± 0.402
3.33AlaAsn: 3.33 ± 0.551
4.515AlaPro: 4.515 ± 0.395
4.459AlaGln: 4.459 ± 0.578
6.152AlaArg: 6.152 ± 0.623
6.039AlaSer: 6.039 ± 0.704
4.628AlaThr: 4.628 ± 0.516
6.208AlaVal: 6.208 ± 0.619
1.806AlaTrp: 1.806 ± 0.352
3.104AlaTyr: 3.104 ± 0.502
0.0AlaXaa: 0.0 ± 0.0
Cys
0.564CysAla: 0.564 ± 0.149
0.169CysCys: 0.169 ± 0.107
0.452CysAsp: 0.452 ± 0.14
0.621CysGlu: 0.621 ± 0.188
0.226CysPhe: 0.226 ± 0.102
1.129CysGly: 1.129 ± 0.249
0.564CysHis: 0.564 ± 0.187
0.564CysIle: 0.564 ± 0.167
0.452CysLys: 0.452 ± 0.164
1.016CysLeu: 1.016 ± 0.224
0.056CysMet: 0.056 ± 0.062
0.282CysAsn: 0.282 ± 0.142
1.072CysPro: 1.072 ± 0.264
0.339CysGln: 0.339 ± 0.131
0.621CysArg: 0.621 ± 0.173
0.677CysSer: 0.677 ± 0.183
0.677CysThr: 0.677 ± 0.194
0.677CysVal: 0.677 ± 0.179
0.395CysTrp: 0.395 ± 0.156
0.508CysTyr: 0.508 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
4.628AspAla: 4.628 ± 0.544
0.847AspCys: 0.847 ± 0.224
3.161AspAsp: 3.161 ± 0.526
5.136AspGlu: 5.136 ± 0.495
2.145AspPhe: 2.145 ± 0.316
4.572AspGly: 4.572 ± 0.386
0.79AspHis: 0.79 ± 0.212
2.596AspIle: 2.596 ± 0.426
2.54AspLys: 2.54 ± 0.31
5.362AspLeu: 5.362 ± 0.477
1.637AspMet: 1.637 ± 0.256
2.258AspAsn: 2.258 ± 0.356
3.669AspPro: 3.669 ± 0.453
1.975AspGln: 1.975 ± 0.356
3.838AspArg: 3.838 ± 0.481
2.653AspSer: 2.653 ± 0.329
2.653AspThr: 2.653 ± 0.447
3.386AspVal: 3.386 ± 0.407
1.242AspTrp: 1.242 ± 0.261
1.58AspTyr: 1.58 ± 0.35
0.0AspXaa: 0.0 ± 0.0
Glu
8.748GluAla: 8.748 ± 0.742
1.016GluCys: 1.016 ± 0.241
4.064GluAsp: 4.064 ± 0.671
6.716GluGlu: 6.716 ± 0.779
3.443GluPhe: 3.443 ± 0.399
5.588GluGly: 5.588 ± 0.505
1.242GluHis: 1.242 ± 0.256
2.314GluIle: 2.314 ± 0.322
3.669GluLys: 3.669 ± 0.511
9.256GluLeu: 9.256 ± 0.838
2.37GluMet: 2.37 ± 0.411
2.088GluAsn: 2.088 ± 0.325
2.314GluPro: 2.314 ± 0.313
2.822GluGln: 2.822 ± 0.439
5.362GluArg: 5.362 ± 0.575
3.499GluSer: 3.499 ± 0.504
3.781GluThr: 3.781 ± 0.512
5.87GluVal: 5.87 ± 0.528
1.75GluTrp: 1.75 ± 0.321
1.863GluTyr: 1.863 ± 0.316
0.0GluXaa: 0.0 ± 0.0
Phe
2.822PheAla: 2.822 ± 0.425
0.452PheCys: 0.452 ± 0.166
2.709PheAsp: 2.709 ± 0.367
2.709PheGlu: 2.709 ± 0.348
1.524PhePhe: 1.524 ± 0.271
2.991PheGly: 2.991 ± 0.45
0.621PheHis: 0.621 ± 0.175
1.129PheIle: 1.129 ± 0.249
1.806PheLys: 1.806 ± 0.41
2.878PheLeu: 2.878 ± 0.348
0.734PheMet: 0.734 ± 0.206
1.185PheAsn: 1.185 ± 0.25
1.919PhePro: 1.919 ± 0.323
1.411PheGln: 1.411 ± 0.243
3.217PheArg: 3.217 ± 0.41
1.863PheSer: 1.863 ± 0.311
2.483PheThr: 2.483 ± 0.359
2.032PheVal: 2.032 ± 0.275
0.734PheTrp: 0.734 ± 0.222
1.072PheTyr: 1.072 ± 0.326
0.0PheXaa: 0.0 ± 0.0
Gly
6.378GlyAla: 6.378 ± 0.591
1.072GlyCys: 1.072 ± 0.313
4.346GlyAsp: 4.346 ± 0.529
5.475GlyGlu: 5.475 ± 0.505
2.878GlyPhe: 2.878 ± 0.358
6.603GlyGly: 6.603 ± 0.597
1.185GlyHis: 1.185 ± 0.252
3.161GlyIle: 3.161 ± 0.429
5.08GlyLys: 5.08 ± 0.506
6.547GlyLeu: 6.547 ± 0.771
1.75GlyMet: 1.75 ± 0.307
2.596GlyAsn: 2.596 ± 0.336
2.935GlyPro: 2.935 ± 0.462
2.878GlyGln: 2.878 ± 0.424
5.588GlyArg: 5.588 ± 0.497
5.249GlySer: 5.249 ± 0.868
4.628GlyThr: 4.628 ± 0.7
5.305GlyVal: 5.305 ± 0.618
2.201GlyTrp: 2.201 ± 0.334
2.709GlyTyr: 2.709 ± 0.518
0.0GlyXaa: 0.0 ± 0.0
His
1.524HisAla: 1.524 ± 0.26
0.282HisCys: 0.282 ± 0.159
0.903HisAsp: 0.903 ± 0.212
1.129HisGlu: 1.129 ± 0.254
1.072HisPhe: 1.072 ± 0.256
1.975HisGly: 1.975 ± 0.352
0.169HisHis: 0.169 ± 0.097
1.072HisIle: 1.072 ± 0.289
0.847HisLys: 0.847 ± 0.229
1.919HisLeu: 1.919 ± 0.404
0.564HisMet: 0.564 ± 0.181
0.508HisAsn: 0.508 ± 0.166
0.903HisPro: 0.903 ± 0.232
0.564HisGln: 0.564 ± 0.198
1.58HisArg: 1.58 ± 0.276
0.79HisSer: 0.79 ± 0.174
1.072HisThr: 1.072 ± 0.251
1.637HisVal: 1.637 ± 0.305
0.734HisTrp: 0.734 ± 0.196
0.847HisTyr: 0.847 ± 0.23
0.0HisXaa: 0.0 ± 0.0
Ile
3.838IleAla: 3.838 ± 0.546
0.339IleCys: 0.339 ± 0.14
3.274IleAsp: 3.274 ± 0.428
3.556IleGlu: 3.556 ± 0.491
0.79IlePhe: 0.79 ± 0.164
2.653IleGly: 2.653 ± 0.387
1.242IleHis: 1.242 ± 0.296
1.524IleIle: 1.524 ± 0.349
1.467IleLys: 1.467 ± 0.297
3.217IleLeu: 3.217 ± 0.5
0.734IleMet: 0.734 ± 0.199
1.863IleAsn: 1.863 ± 0.312
1.975IlePro: 1.975 ± 0.337
1.919IleGln: 1.919 ± 0.364
2.596IleArg: 2.596 ± 0.436
2.258IleSer: 2.258 ± 0.32
1.75IleThr: 1.75 ± 0.365
2.54IleVal: 2.54 ± 0.434
0.508IleTrp: 0.508 ± 0.17
0.959IleTyr: 0.959 ± 0.226
0.0IleXaa: 0.0 ± 0.0
Lys
5.983LysAla: 5.983 ± 0.566
0.113LysCys: 0.113 ± 0.111
2.991LysAsp: 2.991 ± 0.393
3.33LysGlu: 3.33 ± 0.512
1.693LysPhe: 1.693 ± 0.309
3.274LysGly: 3.274 ± 0.465
1.242LysHis: 1.242 ± 0.288
1.467LysIle: 1.467 ± 0.278
2.427LysLys: 2.427 ± 0.479
4.797LysLeu: 4.797 ± 0.604
1.242LysMet: 1.242 ± 0.197
1.693LysAsn: 1.693 ± 0.284
1.919LysPro: 1.919 ± 0.327
2.088LysGln: 2.088 ± 0.345
2.766LysArg: 2.766 ± 0.384
2.258LysSer: 2.258 ± 0.331
3.612LysThr: 3.612 ± 0.589
3.951LysVal: 3.951 ± 0.516
0.564LysTrp: 0.564 ± 0.159
1.355LysTyr: 1.355 ± 0.213
0.0LysXaa: 0.0 ± 0.0
Leu
9.256LeuAla: 9.256 ± 0.71
0.903LeuCys: 0.903 ± 0.223
5.305LeuAsp: 5.305 ± 0.601
6.716LeuGlu: 6.716 ± 0.651
2.991LeuPhe: 2.991 ± 0.407
7.45LeuGly: 7.45 ± 0.622
1.75LeuHis: 1.75 ± 0.285
3.386LeuIle: 3.386 ± 0.482
4.459LeuLys: 4.459 ± 0.459
8.974LeuLeu: 8.974 ± 0.88
2.201LeuMet: 2.201 ± 0.342
3.725LeuAsn: 3.725 ± 0.42
4.854LeuPro: 4.854 ± 0.708
3.894LeuGln: 3.894 ± 0.425
6.66LeuArg: 6.66 ± 0.615
5.136LeuSer: 5.136 ± 0.59
5.08LeuThr: 5.08 ± 0.495
6.491LeuVal: 6.491 ± 0.59
1.524LeuTrp: 1.524 ± 0.293
2.314LeuTyr: 2.314 ± 0.362
0.0LeuXaa: 0.0 ± 0.0
Met
3.217MetAla: 3.217 ± 0.394
0.113MetCys: 0.113 ± 0.075
1.185MetAsp: 1.185 ± 0.204
1.411MetGlu: 1.411 ± 0.306
0.339MetPhe: 0.339 ± 0.152
1.524MetGly: 1.524 ± 0.437
0.395MetHis: 0.395 ± 0.167
0.79MetIle: 0.79 ± 0.272
1.072MetLys: 1.072 ± 0.244
1.355MetLeu: 1.355 ± 0.262
0.282MetMet: 0.282 ± 0.124
0.734MetAsn: 0.734 ± 0.246
1.129MetPro: 1.129 ± 0.246
0.734MetGln: 0.734 ± 0.183
1.072MetArg: 1.072 ± 0.183
1.693MetSer: 1.693 ± 0.294
2.314MetThr: 2.314 ± 0.364
1.129MetVal: 1.129 ± 0.254
0.339MetTrp: 0.339 ± 0.13
0.169MetTyr: 0.169 ± 0.098
0.0MetXaa: 0.0 ± 0.0
Asn
2.878AsnAla: 2.878 ± 0.313
0.452AsnCys: 0.452 ± 0.145
1.863AsnAsp: 1.863 ± 0.287
1.919AsnGlu: 1.919 ± 0.342
1.129AsnPhe: 1.129 ± 0.239
3.612AsnGly: 3.612 ± 0.445
0.734AsnHis: 0.734 ± 0.201
1.355AsnIle: 1.355 ± 0.261
1.242AsnLys: 1.242 ± 0.255
2.935AsnLeu: 2.935 ± 0.378
0.564AsnMet: 0.564 ± 0.161
0.734AsnAsn: 0.734 ± 0.192
2.766AsnPro: 2.766 ± 0.37
1.524AsnGln: 1.524 ± 0.295
2.653AsnArg: 2.653 ± 0.357
1.411AsnSer: 1.411 ± 0.373
1.298AsnThr: 1.298 ± 0.309
2.991AsnVal: 2.991 ± 0.631
1.072AsnTrp: 1.072 ± 0.2
0.903AsnTyr: 0.903 ± 0.198
0.0AsnXaa: 0.0 ± 0.0
Pro
4.854ProAla: 4.854 ± 0.497
0.847ProCys: 0.847 ± 0.191
3.274ProAsp: 3.274 ± 0.516
4.515ProGlu: 4.515 ± 0.439
2.201ProPhe: 2.201 ± 0.358
4.289ProGly: 4.289 ± 0.573
0.621ProHis: 0.621 ± 0.17
1.411ProIle: 1.411 ± 0.321
2.032ProLys: 2.032 ± 0.366
3.556ProLeu: 3.556 ± 0.507
0.847ProMet: 0.847 ± 0.263
1.75ProAsn: 1.75 ± 0.324
2.314ProPro: 2.314 ± 0.417
1.693ProGln: 1.693 ± 0.264
2.37ProArg: 2.37 ± 0.387
3.217ProSer: 3.217 ± 0.354
2.766ProThr: 2.766 ± 0.447
3.161ProVal: 3.161 ± 0.689
1.016ProTrp: 1.016 ± 0.279
1.806ProTyr: 1.806 ± 0.386
0.0ProXaa: 0.0 ± 0.0
Gln
4.177GlnAla: 4.177 ± 0.444
0.282GlnCys: 0.282 ± 0.112
2.145GlnAsp: 2.145 ± 0.332
3.386GlnGlu: 3.386 ± 0.353
1.58GlnPhe: 1.58 ± 0.404
2.991GlnGly: 2.991 ± 0.323
0.959GlnHis: 0.959 ± 0.223
1.58GlnIle: 1.58 ± 0.297
1.355GlnLys: 1.355 ± 0.257
4.572GlnLeu: 4.572 ± 0.544
1.129GlnMet: 1.129 ± 0.252
1.355GlnAsn: 1.355 ± 0.327
1.693GlnPro: 1.693 ± 0.356
2.088GlnGln: 2.088 ± 0.477
2.54GlnArg: 2.54 ± 0.442
1.806GlnSer: 1.806 ± 0.302
1.467GlnThr: 1.467 ± 0.28
2.709GlnVal: 2.709 ± 0.422
0.621GlnTrp: 0.621 ± 0.168
1.298GlnTyr: 1.298 ± 0.296
0.0GlnXaa: 0.0 ± 0.0
Arg
5.531ArgAla: 5.531 ± 0.592
0.677ArgCys: 0.677 ± 0.203
3.781ArgAsp: 3.781 ± 0.376
5.983ArgGlu: 5.983 ± 0.6
2.088ArgPhe: 2.088 ± 0.42
4.289ArgGly: 4.289 ± 0.415
2.201ArgHis: 2.201 ± 0.39
3.443ArgIle: 3.443 ± 0.421
3.499ArgLys: 3.499 ± 0.395
7.224ArgLeu: 7.224 ± 0.763
1.242ArgMet: 1.242 ± 0.272
2.032ArgAsn: 2.032 ± 0.337
3.556ArgPro: 3.556 ± 0.469
2.822ArgGln: 2.822 ± 0.432
5.7ArgArg: 5.7 ± 0.638
3.217ArgSer: 3.217 ± 0.403
2.935ArgThr: 2.935 ± 0.433
4.854ArgVal: 4.854 ± 0.596
1.637ArgTrp: 1.637 ± 0.309
1.863ArgTyr: 1.863 ± 0.321
0.0ArgXaa: 0.0 ± 0.0
Ser
4.177SerAla: 4.177 ± 0.446
0.564SerCys: 0.564 ± 0.2
2.37SerAsp: 2.37 ± 0.402
3.838SerGlu: 3.838 ± 0.438
2.145SerPhe: 2.145 ± 0.407
5.023SerGly: 5.023 ± 0.654
1.072SerHis: 1.072 ± 0.262
2.766SerIle: 2.766 ± 0.402
3.161SerLys: 3.161 ± 0.345
4.854SerLeu: 4.854 ± 0.568
1.298SerMet: 1.298 ± 0.245
2.088SerAsn: 2.088 ± 0.383
2.088SerPro: 2.088 ± 0.374
2.483SerGln: 2.483 ± 0.477
3.951SerArg: 3.951 ± 0.495
3.894SerSer: 3.894 ± 0.569
2.878SerThr: 2.878 ± 0.426
4.12SerVal: 4.12 ± 0.504
1.637SerTrp: 1.637 ± 0.335
1.806SerTyr: 1.806 ± 0.331
0.0SerXaa: 0.0 ± 0.0
Thr
5.249ThrAla: 5.249 ± 0.472
0.677ThrCys: 0.677 ± 0.187
2.709ThrAsp: 2.709 ± 0.402
3.556ThrGlu: 3.556 ± 0.44
2.54ThrPhe: 2.54 ± 0.405
4.515ThrGly: 4.515 ± 0.699
1.185ThrHis: 1.185 ± 0.246
2.37ThrIle: 2.37 ± 0.335
2.766ThrLys: 2.766 ± 0.319
4.572ThrLeu: 4.572 ± 0.508
0.621ThrMet: 0.621 ± 0.203
1.524ThrAsn: 1.524 ± 0.279
3.443ThrPro: 3.443 ± 0.496
1.919ThrGln: 1.919 ± 0.39
2.935ThrArg: 2.935 ± 0.453
3.274ThrSer: 3.274 ± 0.53
3.161ThrThr: 3.161 ± 0.51
4.064ThrVal: 4.064 ± 0.562
0.847ThrTrp: 0.847 ± 0.242
1.975ThrTyr: 1.975 ± 0.353
0.0ThrXaa: 0.0 ± 0.0
Val
7.337ValAla: 7.337 ± 0.542
0.452ValCys: 0.452 ± 0.155
3.951ValAsp: 3.951 ± 0.57
5.362ValGlu: 5.362 ± 0.562
2.653ValPhe: 2.653 ± 0.343
5.249ValGly: 5.249 ± 0.541
1.411ValHis: 1.411 ± 0.299
2.201ValIle: 2.201 ± 0.324
3.725ValLys: 3.725 ± 0.412
6.095ValLeu: 6.095 ± 0.644
0.959ValMet: 0.959 ± 0.256
1.975ValAsn: 1.975 ± 0.374
2.991ValPro: 2.991 ± 0.421
2.709ValGln: 2.709 ± 0.388
5.192ValArg: 5.192 ± 0.59
4.402ValSer: 4.402 ± 0.499
4.572ValThr: 4.572 ± 0.626
5.418ValVal: 5.418 ± 0.503
1.58ValTrp: 1.58 ± 0.313
1.411ValTyr: 1.411 ± 0.276
0.0ValXaa: 0.0 ± 0.0
Trp
2.709TrpAla: 2.709 ± 0.344
0.395TrpCys: 0.395 ± 0.148
1.467TrpAsp: 1.467 ± 0.311
1.919TrpGlu: 1.919 ± 0.372
0.677TrpPhe: 0.677 ± 0.177
1.185TrpGly: 1.185 ± 0.272
0.169TrpHis: 0.169 ± 0.093
0.79TrpIle: 0.79 ± 0.222
1.185TrpLys: 1.185 ± 0.252
2.088TrpLeu: 2.088 ± 0.335
0.508TrpMet: 0.508 ± 0.156
0.903TrpAsn: 0.903 ± 0.226
1.129TrpPro: 1.129 ± 0.223
0.621TrpGln: 0.621 ± 0.178
1.411TrpArg: 1.411 ± 0.367
1.016TrpSer: 1.016 ± 0.255
0.847TrpThr: 0.847 ± 0.217
1.016TrpVal: 1.016 ± 0.245
0.282TrpTrp: 0.282 ± 0.141
0.847TrpTyr: 0.847 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.54TyrAla: 2.54 ± 0.478
0.452TyrCys: 0.452 ± 0.161
1.863TyrAsp: 1.863 ± 0.32
2.258TyrGlu: 2.258 ± 0.352
0.79TyrPhe: 0.79 ± 0.235
2.766TyrGly: 2.766 ± 0.4
0.508TyrHis: 0.508 ± 0.166
1.355TyrIle: 1.355 ± 0.33
0.847TyrLys: 0.847 ± 0.221
2.709TyrLeu: 2.709 ± 0.412
0.395TyrMet: 0.395 ± 0.174
1.411TyrAsn: 1.411 ± 0.246
1.355TyrPro: 1.355 ± 0.266
0.79TyrGln: 0.79 ± 0.197
2.427TyrArg: 2.427 ± 0.365
1.863TyrSer: 1.863 ± 0.441
1.298TyrThr: 1.298 ± 0.287
2.088TyrVal: 2.088 ± 0.287
0.677TyrTrp: 0.677 ± 0.181
0.734TyrTyr: 0.734 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (17719 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski