Amino acid dipepetide frequency for Daejeonella rubra

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.652AlaAla: 5.652 ± 0.095	0.607AlaCys: 0.607 ± 0.025	4.028AlaAsp: 4.028 ± 0.058	4.591AlaGlu: 4.591 ± 0.068	3.435AlaPhe: 3.435 ± 0.049	5.635AlaGly: 5.635 ± 0.08	1.118AlaHis: 1.118 ± 0.034	5.565AlaIle: 5.565 ± 0.071	4.42AlaLys: 4.42 ± 0.068	6.468AlaLeu: 6.468 ± 0.069	1.66AlaMet: 1.66 ± 0.035	3.395AlaAsn: 3.395 ± 0.068	2.157AlaPro: 2.157 ± 0.05	2.465AlaGln: 2.465 ± 0.048	2.517AlaArg: 2.517 ± 0.043	4.649AlaSer: 4.649 ± 0.066	3.311AlaThr: 3.311 ± 0.075	4.435AlaVal: 4.435 ± 0.063	0.753AlaTrp: 0.753 ± 0.028	2.503AlaTyr: 2.503 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.457CysAla: 0.457 ± 0.024	0.118CysCys: 0.118 ± 0.01	0.402CysAsp: 0.402 ± 0.02	0.4CysGlu: 0.4 ± 0.02	0.4CysPhe: 0.4 ± 0.018	0.634CysGly: 0.634 ± 0.026	0.199CysHis: 0.199 ± 0.014	0.666CysIle: 0.666 ± 0.023	0.436CysLys: 0.436 ± 0.017	0.727CysLeu: 0.727 ± 0.024	0.169CysMet: 0.169 ± 0.012	0.374CysAsn: 0.374 ± 0.019	0.347CysPro: 0.347 ± 0.02	0.248CysGln: 0.248 ± 0.014	0.299CysArg: 0.299 ± 0.017	0.601CysSer: 0.601 ± 0.021	0.41CysThr: 0.41 ± 0.018	0.397CysVal: 0.397 ± 0.018	0.082CysTrp: 0.082 ± 0.008	0.276CysTyr: 0.276 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.545AspAla: 3.545 ± 0.05	0.371AspCys: 0.371 ± 0.018	2.377AspAsp: 2.377 ± 0.049	3.296AspGlu: 3.296 ± 0.057	3.136AspPhe: 3.136 ± 0.055	3.651AspGly: 3.651 ± 0.058	1.107AspHis: 1.107 ± 0.029	4.262AspIle: 4.262 ± 0.063	3.789AspLys: 3.789 ± 0.056	5.54AspLeu: 5.54 ± 0.069	1.283AspMet: 1.283 ± 0.034	2.573AspAsn: 2.573 ± 0.053	2.354AspPro: 2.354 ± 0.041	2.089AspGln: 2.089 ± 0.043	2.235AspArg: 2.235 ± 0.047	3.128AspSer: 3.128 ± 0.058	2.414AspThr: 2.414 ± 0.046	3.138AspVal: 3.138 ± 0.047	0.731AspTrp: 0.731 ± 0.024	2.172AspTyr: 2.172 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	4.074GluAla: 4.074 ± 0.066	0.352GluCys: 0.352 ± 0.019	2.791GluAsp: 2.791 ± 0.06	4.009GluGlu: 4.009 ± 0.078	2.792GluPhe: 2.792 ± 0.05	3.535GluGly: 3.535 ± 0.054	1.091GluHis: 1.091 ± 0.032	5.091GluIle: 5.091 ± 0.07	5.14GluLys: 5.14 ± 0.073	6.338GluLeu: 6.338 ± 0.095	1.607GluMet: 1.607 ± 0.039	3.675GluAsn: 3.675 ± 0.064	1.695GluPro: 1.695 ± 0.039	2.238GluGln: 2.238 ± 0.045	2.629GluArg: 2.629 ± 0.043	3.406GluSer: 3.406 ± 0.053	2.909GluThr: 2.909 ± 0.045	3.749GluVal: 3.749 ± 0.059	0.681GluTrp: 0.681 ± 0.021	2.198GluTyr: 2.198 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	3.299PheAla: 3.299 ± 0.052	0.447PheCys: 0.447 ± 0.02	2.845PheAsp: 2.845 ± 0.043	2.935PheGlu: 2.935 ± 0.053	2.457PhePhe: 2.457 ± 0.053	3.485PheGly: 3.485 ± 0.061	0.84PheHis: 0.84 ± 0.027	3.968PheIle: 3.968 ± 0.061	3.483PheLys: 3.483 ± 0.055	4.67PheLeu: 4.67 ± 0.073	1.115PheMet: 1.115 ± 0.031	3.091PheAsn: 3.091 ± 0.059	1.793PhePro: 1.793 ± 0.037	1.486PheGln: 1.486 ± 0.036	1.957PheArg: 1.957 ± 0.037	4.125PheSer: 4.125 ± 0.069	2.89PheThr: 2.89 ± 0.063	2.723PheVal: 2.723 ± 0.048	0.57PheTrp: 0.57 ± 0.024	1.979PheTyr: 1.979 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.379GlyAla: 4.379 ± 0.069	0.627GlyCys: 0.627 ± 0.03	3.339GlyAsp: 3.339 ± 0.052	3.356GlyGlu: 3.356 ± 0.055	3.732GlyPhe: 3.732 ± 0.057	5.104GlyGly: 5.104 ± 0.1	1.307GlyHis: 1.307 ± 0.038	6.051GlyIle: 6.051 ± 0.076	5.414GlyLys: 5.414 ± 0.073	6.517GlyLeu: 6.517 ± 0.078	1.974GlyMet: 1.974 ± 0.041	3.746GlyAsn: 3.746 ± 0.06	1.802GlyPro: 1.802 ± 0.042	2.181GlyGln: 2.181 ± 0.042	2.71GlyArg: 2.71 ± 0.048	4.969GlySer: 4.969 ± 0.074	4.267GlyThr: 4.267 ± 0.098	4.176GlyVal: 4.176 ± 0.067	0.906GlyTrp: 0.906 ± 0.027	2.852GlyTyr: 2.852 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	1.118HisAla: 1.118 ± 0.03	0.199HisCys: 0.199 ± 0.012	0.872HisAsp: 0.872 ± 0.029	0.946HisGlu: 0.946 ± 0.029	1.084HisPhe: 1.084 ± 0.03	1.174HisGly: 1.174 ± 0.031	0.476HisHis: 0.476 ± 0.018	1.333HisIle: 1.333 ± 0.034	0.966HisLys: 0.966 ± 0.028	1.748HisLeu: 1.748 ± 0.036	0.391HisMet: 0.391 ± 0.017	0.822HisAsn: 0.822 ± 0.023	0.985HisPro: 0.985 ± 0.028	0.708HisGln: 0.708 ± 0.024	0.727HisArg: 0.727 ± 0.025	1.163HisSer: 1.163 ± 0.029	0.915HisThr: 0.915 ± 0.03	0.966HisVal: 0.966 ± 0.03	0.228HisTrp: 0.228 ± 0.014	0.763HisTyr: 0.763 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.851IleAla: 5.851 ± 0.081	0.723IleCys: 0.723 ± 0.026	4.502IleAsp: 4.502 ± 0.054	4.786IleGlu: 4.786 ± 0.067	3.567IlePhe: 3.567 ± 0.058	5.241IleGly: 5.241 ± 0.071	1.379IleHis: 1.379 ± 0.032	6.035IleIle: 6.035 ± 0.082	5.422IleLys: 5.422 ± 0.074	7.529IleLeu: 7.529 ± 0.076	1.653IleMet: 1.653 ± 0.045	4.464IleAsn: 4.464 ± 0.07	3.545IlePro: 3.545 ± 0.052	2.5IleGln: 2.5 ± 0.04	3.494IleArg: 3.494 ± 0.052	6.412IleSer: 6.412 ± 0.076	4.278IleThr: 4.278 ± 0.075	4.49IleVal: 4.49 ± 0.06	0.772IleTrp: 0.772 ± 0.024	2.753IleTyr: 2.753 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	4.993LysAla: 4.993 ± 0.065	0.364LysCys: 0.364 ± 0.02	4.083LysAsp: 4.083 ± 0.062	4.825LysGlu: 4.825 ± 0.082	2.925LysPhe: 2.925 ± 0.051	4.562LysGly: 4.562 ± 0.068	1.192LysHis: 1.192 ± 0.03	5.69LysIle: 5.69 ± 0.077	5.557LysLys: 5.557 ± 0.08	6.536LysLeu: 6.536 ± 0.08	1.983LysMet: 1.983 ± 0.045	4.475LysAsn: 4.475 ± 0.064	2.757LysPro: 2.757 ± 0.057	2.552LysGln: 2.552 ± 0.049	2.878LysArg: 2.878 ± 0.052	4.583LysSer: 4.583 ± 0.065	4.047LysThr: 4.047 ± 0.056	4.472LysVal: 4.472 ± 0.056	0.742LysTrp: 0.742 ± 0.027	2.722LysTyr: 2.722 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	6.674LeuAla: 6.674 ± 0.079	0.719LeuCys: 0.719 ± 0.027	4.795LeuAsp: 4.795 ± 0.065	5.422LeuGlu: 5.422 ± 0.079	4.873LeuPhe: 4.873 ± 0.073	5.891LeuGly: 5.891 ± 0.079	1.583LeuHis: 1.583 ± 0.039	7.843LeuIle: 7.843 ± 0.096	7.625LeuLys: 7.625 ± 0.085	8.972LeuLeu: 8.972 ± 0.1	2.326LeuMet: 2.326 ± 0.047	6.019LeuAsn: 6.019 ± 0.072	3.977LeuPro: 3.977 ± 0.066	3.058LeuGln: 3.058 ± 0.052	3.708LeuArg: 3.708 ± 0.061	7.584LeuSer: 7.584 ± 0.084	5.12LeuThr: 5.12 ± 0.062	5.288LeuVal: 5.288 ± 0.068	0.873LeuTrp: 0.873 ± 0.027	3.057LeuTyr: 3.057 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	1.856MetAla: 1.856 ± 0.041	0.15MetCys: 0.15 ± 0.011	1.349MetAsp: 1.349 ± 0.03	1.497MetGlu: 1.497 ± 0.037	0.925MetPhe: 0.925 ± 0.029	1.736MetGly: 1.736 ± 0.04	0.453MetHis: 0.453 ± 0.017	1.872MetIle: 1.872 ± 0.039	2.195MetLys: 2.195 ± 0.037	2.222MetLeu: 2.222 ± 0.044	0.628MetMet: 0.628 ± 0.025	1.493MetAsn: 1.493 ± 0.036	1.072MetPro: 1.072 ± 0.028	0.876MetGln: 0.876 ± 0.025	0.966MetArg: 0.966 ± 0.03	1.493MetSer: 1.493 ± 0.035	1.148MetThr: 1.148 ± 0.031	1.469MetVal: 1.469 ± 0.04	0.19MetTrp: 0.19 ± 0.013	0.665MetTyr: 0.665 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.668AsnAla: 3.668 ± 0.068	0.413AsnCys: 0.413 ± 0.019	2.676AsnAsp: 2.676 ± 0.051	3.21AsnGlu: 3.21 ± 0.052	2.844AsnPhe: 2.844 ± 0.05	3.953AsnGly: 3.953 ± 0.066	0.901AsnHis: 0.901 ± 0.029	4.425AsnIle: 4.425 ± 0.063	3.939AsnLys: 3.939 ± 0.055	5.166AsnLeu: 5.166 ± 0.074	1.349AsnMet: 1.349 ± 0.033	3.254AsnAsn: 3.254 ± 0.067	2.733AsnPro: 2.733 ± 0.05	2.004AsnGln: 2.004 ± 0.051	2.415AsnArg: 2.415 ± 0.045	4.043AsnSer: 4.043 ± 0.067	3.189AsnThr: 3.189 ± 0.061	3.056AsnVal: 3.056 ± 0.054	0.748AsnTrp: 0.748 ± 0.027	2.491AsnTyr: 2.491 ± 0.052	0.0AsnXaa: 0.0 ± 0.0
Pro	3.045ProAla: 3.045 ± 0.054	0.222ProCys: 0.222 ± 0.014	2.623ProAsp: 2.623 ± 0.047	3.168ProGlu: 3.168 ± 0.051	1.943ProPhe: 1.943 ± 0.035	3.011ProGly: 3.011 ± 0.057	0.647ProHis: 0.647 ± 0.026	2.435ProIle: 2.435 ± 0.047	2.24ProLys: 2.24 ± 0.044	3.364ProLeu: 3.364 ± 0.063	0.841ProMet: 0.841 ± 0.027	1.88ProAsn: 1.88 ± 0.041	1.023ProPro: 1.023 ± 0.045	1.221ProGln: 1.221 ± 0.033	1.215ProArg: 1.215 ± 0.029	2.41ProSer: 2.41 ± 0.046	1.666ProThr: 1.666 ± 0.038	3.282ProVal: 3.282 ± 0.054	0.387ProTrp: 0.387 ± 0.017	1.417ProTyr: 1.417 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.389GlnAla: 2.389 ± 0.043	0.189GlnCys: 0.189 ± 0.013	1.625GlnAsp: 1.625 ± 0.042	1.968GlnGlu: 1.968 ± 0.044	1.619GlnPhe: 1.619 ± 0.037	2.04GlnGly: 2.04 ± 0.043	0.598GlnHis: 0.598 ± 0.022	2.701GlnIle: 2.701 ± 0.054	2.695GlnLys: 2.695 ± 0.05	3.351GlnLeu: 3.351 ± 0.061	0.868GlnMet: 0.868 ± 0.029	2.1GlnAsn: 2.1 ± 0.045	1.131GlnPro: 1.131 ± 0.027	1.39GlnGln: 1.39 ± 0.039	1.323GlnArg: 1.323 ± 0.035	2.248GlnSer: 2.248 ± 0.043	1.945GlnThr: 1.945 ± 0.044	2.15GlnVal: 2.15 ± 0.042	0.37GlnTrp: 0.37 ± 0.017	1.269GlnTyr: 1.269 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	2.461ArgAla: 2.461 ± 0.05	0.233ArgCys: 0.233 ± 0.014	2.131ArgAsp: 2.131 ± 0.046	2.461ArgGlu: 2.461 ± 0.047	2.089ArgPhe: 2.089 ± 0.043	2.374ArgGly: 2.374 ± 0.04	0.647ArgHis: 0.647 ± 0.025	3.46ArgIle: 3.46 ± 0.043	2.992ArgLys: 2.992 ± 0.044	3.813ArgLeu: 3.813 ± 0.06	1.102ArgMet: 1.102 ± 0.029	2.424ArgAsn: 2.424 ± 0.05	1.474ArgPro: 1.474 ± 0.037	1.366ArgGln: 1.366 ± 0.033	1.679ArgArg: 1.679 ± 0.038	2.674ArgSer: 2.674 ± 0.051	2.162ArgThr: 2.162 ± 0.035	2.416ArgVal: 2.416 ± 0.042	0.438ArgTrp: 0.438 ± 0.019	1.65ArgTyr: 1.65 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	4.906SerAla: 4.906 ± 0.07	0.623SerCys: 0.623 ± 0.025	3.765SerAsp: 3.765 ± 0.057	4.044SerGlu: 4.044 ± 0.067	3.849SerPhe: 3.849 ± 0.072	5.876SerGly: 5.876 ± 0.086	1.167SerHis: 1.167 ± 0.029	5.508SerIle: 5.508 ± 0.078	4.703SerLys: 4.703 ± 0.057	6.666SerLeu: 6.666 ± 0.084	1.619SerMet: 1.619 ± 0.04	3.645SerAsn: 3.645 ± 0.066	2.587SerPro: 2.587 ± 0.049	2.192SerGln: 2.192 ± 0.04	2.738SerArg: 2.738 ± 0.055	4.916SerSer: 4.916 ± 0.084	3.716SerThr: 3.716 ± 0.068	4.405SerVal: 4.405 ± 0.064	0.803SerTrp: 0.803 ± 0.03	2.743SerTyr: 2.743 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	3.941ThrAla: 3.941 ± 0.095	0.332ThrCys: 0.332 ± 0.017	3.08ThrAsp: 3.08 ± 0.056	3.173ThrGlu: 3.173 ± 0.049	2.576ThrPhe: 2.576 ± 0.054	4.652ThrGly: 4.652 ± 0.086	0.896ThrHis: 0.896 ± 0.026	4.328ThrIle: 4.328 ± 0.07	3.023ThrLys: 3.023 ± 0.05	4.954ThrLeu: 4.954 ± 0.068	1.078ThrMet: 1.078 ± 0.03	2.62ThrAsn: 2.62 ± 0.059	2.225ThrPro: 2.225 ± 0.043	1.578ThrGln: 1.578 ± 0.036	1.899ThrArg: 1.899 ± 0.046	3.684ThrSer: 3.684 ± 0.065	2.744ThrThr: 2.744 ± 0.069	3.51ThrVal: 3.51 ± 0.073	0.632ThrTrp: 0.632 ± 0.025	2.112ThrTyr: 2.112 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	3.975ValAla: 3.975 ± 0.061	0.529ValCys: 0.529 ± 0.021	3.2ValAsp: 3.2 ± 0.054	3.411ValGlu: 3.411 ± 0.057	3.178ValPhe: 3.178 ± 0.057	3.504ValGly: 3.504 ± 0.067	0.989ValHis: 0.989 ± 0.028	4.875ValIle: 4.875 ± 0.066	4.423ValLys: 4.423 ± 0.073	5.967ValLeu: 5.967 ± 0.076	1.506ValMet: 1.506 ± 0.036	3.599ValAsn: 3.599 ± 0.059	2.411ValPro: 2.411 ± 0.049	1.964ValGln: 1.964 ± 0.035	2.444ValArg: 2.444 ± 0.045	4.717ValSer: 4.717 ± 0.068	3.114ValThr: 3.114 ± 0.068	3.71ValVal: 3.71 ± 0.067	0.664ValTrp: 0.664 ± 0.024	2.299ValTyr: 2.299 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.711TrpAla: 0.711 ± 0.026	0.086TrpCys: 0.086 ± 0.008	0.674TrpAsp: 0.674 ± 0.026	0.628TrpGlu: 0.628 ± 0.021	0.576TrpPhe: 0.576 ± 0.023	0.746TrpGly: 0.746 ± 0.024	0.232TrpHis: 0.232 ± 0.014	0.806TrpIle: 0.806 ± 0.025	0.886TrpLys: 0.886 ± 0.03	1.083TrpLeu: 1.083 ± 0.031	0.359TrpMet: 0.359 ± 0.018	0.649TrpAsn: 0.649 ± 0.023	0.332TrpPro: 0.332 ± 0.019	0.434TrpGln: 0.434 ± 0.016	0.443TrpArg: 0.443 ± 0.021	0.71TrpSer: 0.71 ± 0.025	0.609TrpThr: 0.609 ± 0.024	0.649TrpVal: 0.649 ± 0.025	0.166TrpTrp: 0.166 ± 0.012	0.431TrpTyr: 0.431 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.367TyrAla: 2.367 ± 0.041	0.336TyrCys: 0.336 ± 0.017	2.037TyrAsp: 2.037 ± 0.038	1.873TyrGlu: 1.873 ± 0.045	2.154TyrPhe: 2.154 ± 0.044	2.605TyrGly: 2.605 ± 0.051	0.764TyrHis: 0.764 ± 0.025	2.482TyrIle: 2.482 ± 0.041	2.591TyrLys: 2.591 ± 0.047	3.774TyrLeu: 3.774 ± 0.063	0.745TyrMet: 0.745 ± 0.025	2.18TyrAsn: 2.18 ± 0.047	1.639TyrPro: 1.639 ± 0.039	1.418TyrGln: 1.418 ± 0.035	1.77TyrArg: 1.77 ± 0.039	2.958TyrSer: 2.958 ± 0.049	2.182TyrThr: 2.182 ± 0.046	2.001TyrVal: 2.001 ± 0.038	0.475TyrTrp: 0.475 ± 0.019	1.571TyrTyr: 1.571 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3697 proteins (1263531 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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