Duwamo virus

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2RVQ1|A0A1B2RVQ1_9VIRU Capsid protein OS=Duwamo virus OX=1888317 PE=4 SV=1
MM1 pKa = 7.71SSTSHH6 pKa = 6.61IPQPAAGATDD16 pKa = 3.7EE17 pKa = 5.16VFDD20 pKa = 4.19STSATAVQPTVSRR33 pKa = 11.84LGIPAGPPQEE43 pKa = 4.64IPIHH47 pKa = 5.42TGQVNTYY54 pKa = 9.23DD55 pKa = 4.57ASIHH59 pKa = 4.92QQWVPTDD66 pKa = 3.95SITWTVSQPSGTLLWFKK83 pKa = 10.09PIHH86 pKa = 5.59PTFSNALLAYY96 pKa = 10.37LSRR99 pKa = 11.84IYY101 pKa = 10.37NAWGGALDD109 pKa = 4.15YY110 pKa = 10.53KK111 pKa = 10.65FKK113 pKa = 10.69IAGTGFHH120 pKa = 7.02AGAIAIVRR128 pKa = 11.84IPPNRR133 pKa = 11.84NPSEE137 pKa = 4.03FTTPQSWGAFEE148 pKa = 5.05YY149 pKa = 10.37MVIDD153 pKa = 4.45PKK155 pKa = 10.62TLEE158 pKa = 4.49TISVGISDD166 pKa = 4.17QRR168 pKa = 11.84PIAYY172 pKa = 9.48HH173 pKa = 4.93YY174 pKa = 10.51FPYY177 pKa = 10.62NGEE180 pKa = 4.12NPLSFGGWIAMYY192 pKa = 10.6VLIPLNTSSSGSQTISIQSFCKK214 pKa = 9.42PAEE217 pKa = 3.96FFQYY221 pKa = 10.37SQLIMPSEE229 pKa = 4.69NISADD234 pKa = 3.51PFPSEE239 pKa = 3.55FAYY242 pKa = 10.51AFDD245 pKa = 3.65FTTAKK250 pKa = 10.38YY251 pKa = 10.26CATAPLYY258 pKa = 10.42CDD260 pKa = 3.39QVTLEE265 pKa = 4.67ANSVTQSFYY274 pKa = 11.39VANCYY279 pKa = 8.78STGGDD284 pKa = 4.0RR285 pKa = 11.84LTKK288 pKa = 9.86WMSKK292 pKa = 9.33GSKK295 pKa = 10.27FPDD298 pKa = 3.04VRR300 pKa = 11.84DD301 pKa = 3.44THH303 pKa = 8.02RR304 pKa = 11.84EE305 pKa = 3.52GKK307 pKa = 7.36TQMRR311 pKa = 11.84ALTDD315 pKa = 3.96LSDD318 pKa = 3.41EE319 pKa = 4.44TIKK322 pKa = 11.14VKK324 pKa = 10.71LYY326 pKa = 10.06FKK328 pKa = 10.53QDD330 pKa = 3.6YY331 pKa = 10.35YY332 pKa = 10.64PIALPASKK340 pKa = 10.36EE341 pKa = 4.18NYY343 pKa = 9.58VSLLTYY349 pKa = 10.53SSEE352 pKa = 4.21NVKK355 pKa = 10.79NEE357 pKa = 3.53AWHH360 pKa = 5.7EE361 pKa = 4.26RR362 pKa = 11.84TWTALYY368 pKa = 10.88SEE370 pKa = 5.92DD371 pKa = 3.75NVAWQCATAATDD383 pKa = 3.26IVTDD387 pKa = 3.79TVVSIQNAKK396 pKa = 10.08FVLDD400 pKa = 4.04PSYY403 pKa = 11.37TDD405 pKa = 3.3NTFSTAVTGEE415 pKa = 4.47SILHH419 pKa = 5.82FEE421 pKa = 4.14NTISKK426 pKa = 9.79VKK428 pKa = 10.42SIQTEE433 pKa = 3.75EE434 pKa = 3.8MAALFSTGRR443 pKa = 11.84VKK445 pKa = 10.45QAFYY449 pKa = 11.52GNMCVLFTMISVDD462 pKa = 3.31EE463 pKa = 4.76DD464 pKa = 4.01LPIGYY469 pKa = 9.97AKK471 pKa = 10.51LYY473 pKa = 10.5QEE475 pKa = 4.99GFLSIRR481 pKa = 11.84VGNKK485 pKa = 7.52QVVLKK490 pKa = 10.07PSNLRR495 pKa = 11.84LKK497 pKa = 10.69FSGFIARR504 pKa = 11.84TDD506 pKa = 4.86PIPSSAEE513 pKa = 3.83YY514 pKa = 9.69NKK516 pKa = 10.31NMYY519 pKa = 8.42MVRR522 pKa = 11.84ALSRR526 pKa = 11.84NN527 pKa = 3.51

Molecular weight: 58.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2RVP9|A0A1B2RVP9_9VIRU RNA-dependent RNA polymerase OS=Duwamo virus OX=1888317 PE=4 SV=1
MM1 pKa = 7.28AAAAIGATAAAGASNIVSSGLTAGLQIYY29 pKa = 10.13GNSLNNSQQFAYY41 pKa = 9.57NDD43 pKa = 3.9SVISRR48 pKa = 11.84AEE50 pKa = 3.69HH51 pKa = 6.58AFTDD55 pKa = 3.65NGLPKK60 pKa = 10.71YY61 pKa = 9.25MAYY64 pKa = 10.04QSNSGTTPTKK74 pKa = 9.87MYY76 pKa = 10.14QISGGNFYY84 pKa = 10.99SAGPVNSNLPMYY96 pKa = 7.65TTTAQQAFHH105 pKa = 6.88AAAPRR110 pKa = 11.84PNARR114 pKa = 11.84VGPGEE119 pKa = 3.76PAIPNWGDD127 pKa = 2.9IHH129 pKa = 8.12NGMPMVPVGQNNRR142 pKa = 11.84QGLGFGRR149 pKa = 11.84YY150 pKa = 8.94NIANNFVQGGPMLNANVRR168 pKa = 11.84NADD171 pKa = 3.19QHH173 pKa = 5.18QAFARR178 pKa = 11.84ALQQ181 pKa = 3.59

Molecular weight: 18.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1355

181

647

451.7

50.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.266 ± 1.57

1.033 ± 0.191

5.166 ± 0.822

4.723 ± 0.799

4.797 ± 0.228

6.052 ± 0.878

3.1 ± 0.821

5.609 ± 0.362

5.83 ± 1.434

7.675 ± 1.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.435 ± 0.251

5.387 ± 1.119

5.535 ± 0.527

4.133 ± 0.701

3.321 ± 0.127

7.159 ± 1.321

7.159 ± 0.675

5.978 ± 0.351

1.771 ± 0.225

4.871 ± 0.111
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski