Amino acid dipepetide frequency for bacterium HR23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.02AlaAla: 5.02 ± 0.138
1.327AlaCys: 1.327 ± 0.053
4.247AlaAsp: 4.247 ± 0.098
5.876AlaGlu: 5.876 ± 0.114
4.289AlaPhe: 4.289 ± 0.11
9.029AlaGly: 9.029 ± 0.165
2.645AlaHis: 2.645 ± 0.076
4.908AlaIle: 4.908 ± 0.097
2.307AlaLys: 2.307 ± 0.075
16.619AlaLeu: 16.619 ± 0.231
2.204AlaMet: 2.204 ± 0.058
1.887AlaAsn: 1.887 ± 0.058
5.889AlaPro: 5.889 ± 0.131
5.627AlaGln: 5.627 ± 0.113
9.215AlaArg: 9.215 ± 0.134
5.521AlaSer: 5.521 ± 0.121
4.934AlaThr: 4.934 ± 0.111
9.921AlaVal: 9.921 ± 0.175
1.703AlaTrp: 1.703 ± 0.068
2.954AlaTyr: 2.954 ± 0.085
0.0AlaXaa: 0.0 ± 0.0
Cys
1.111CysAla: 1.111 ± 0.048
0.101CysCys: 0.101 ± 0.015
0.387CysAsp: 0.387 ± 0.027
0.359CysGlu: 0.359 ± 0.025
0.296CysPhe: 0.296 ± 0.028
1.086CysGly: 1.086 ± 0.056
0.332CysHis: 0.332 ± 0.031
0.503CysIle: 0.503 ± 0.033
0.258CysLys: 0.258 ± 0.022
0.925CysLeu: 0.925 ± 0.041
0.139CysMet: 0.139 ± 0.016
0.222CysAsn: 0.222 ± 0.02
0.894CysPro: 0.894 ± 0.05
0.444CysGln: 0.444 ± 0.029
0.843CysArg: 0.843 ± 0.048
0.463CysSer: 0.463 ± 0.035
0.596CysThr: 0.596 ± 0.04
0.653CysVal: 0.653 ± 0.041
0.154CysTrp: 0.154 ± 0.018
0.296CysTyr: 0.296 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
4.849AspAla: 4.849 ± 0.108
0.357AspCys: 0.357 ± 0.03
1.504AspAsp: 1.504 ± 0.061
2.09AspGlu: 2.09 ± 0.061
1.534AspPhe: 1.534 ± 0.057
3.385AspGly: 3.385 ± 0.103
0.723AspHis: 0.723 ± 0.039
2.483AspIle: 2.483 ± 0.077
1.006AspLys: 1.006 ± 0.049
5.809AspLeu: 5.809 ± 0.117
0.917AspMet: 0.917 ± 0.046
0.729AspAsn: 0.729 ± 0.038
3.387AspPro: 3.387 ± 0.086
1.016AspGln: 1.016 ± 0.05
2.641AspArg: 2.641 ± 0.074
1.219AspSer: 1.219 ± 0.052
2.242AspThr: 2.242 ± 0.073
2.994AspVal: 2.994 ± 0.078
0.543AspTrp: 0.543 ± 0.038
1.559AspTyr: 1.559 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
8.336GluAla: 8.336 ± 0.144
0.48GluCys: 0.48 ± 0.032
2.593GluAsp: 2.593 ± 0.079
5.796GluGlu: 5.796 ± 0.147
1.585GluPhe: 1.585 ± 0.069
6.438GluGly: 6.438 ± 0.121
1.479GluHis: 1.479 ± 0.063
2.121GluIle: 2.121 ± 0.073
2.016GluLys: 2.016 ± 0.072
3.753GluLeu: 3.753 ± 0.1
1.475GluMet: 1.475 ± 0.05
1.054GluAsn: 1.054 ± 0.045
2.821GluPro: 2.821 ± 0.083
2.231GluGln: 2.231 ± 0.073
6.65GluArg: 6.65 ± 0.134
1.76GluSer: 1.76 ± 0.055
2.764GluThr: 2.764 ± 0.075
6.979GluVal: 6.979 ± 0.108
0.82GluTrp: 0.82 ± 0.04
1.111GluTyr: 1.111 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
3.41PheAla: 3.41 ± 0.084
0.48PheCys: 0.48 ± 0.034
1.198PheAsp: 1.198 ± 0.051
1.223PheGlu: 1.223 ± 0.05
1.378PhePhe: 1.378 ± 0.052
2.84PheGly: 2.84 ± 0.075
0.866PheHis: 0.866 ± 0.041
1.54PheIle: 1.54 ± 0.062
0.581PheLys: 0.581 ± 0.036
4.332PheLeu: 4.332 ± 0.101
0.486PheMet: 0.486 ± 0.034
0.771PheAsn: 0.771 ± 0.042
2.293PhePro: 2.293 ± 0.055
1.357PheGln: 1.357 ± 0.054
2.61PheArg: 2.61 ± 0.075
1.747PheSer: 1.747 ± 0.054
2.198PheThr: 2.198 ± 0.078
2.242PheVal: 2.242 ± 0.075
0.668PheTrp: 0.668 ± 0.038
0.997PheTyr: 0.997 ± 0.052
0.0PheXaa: 0.0 ± 0.0
Gly
8.642GlyAla: 8.642 ± 0.159
0.945GlyCys: 0.945 ± 0.057
3.63GlyAsp: 3.63 ± 0.099
5.506GlyGlu: 5.506 ± 0.119
2.973GlyPhe: 2.973 ± 0.075
7.75GlyGly: 7.75 ± 0.184
2.092GlyHis: 2.092 ± 0.068
4.735GlyIle: 4.735 ± 0.108
3.387GlyLys: 3.387 ± 0.086
9.625GlyLeu: 9.625 ± 0.159
2.624GlyMet: 2.624 ± 0.07
1.688GlyAsn: 1.688 ± 0.079
4.224GlyPro: 4.224 ± 0.087
3.503GlyGln: 3.503 ± 0.103
7.357GlyArg: 7.357 ± 0.138
3.694GlySer: 3.694 ± 0.092
4.444GlyThr: 4.444 ± 0.098
8.845GlyVal: 8.845 ± 0.147
1.745GlyTrp: 1.745 ± 0.074
2.61GlyTyr: 2.61 ± 0.076
0.0GlyXaa: 0.0 ± 0.0
His
2.121HisAla: 2.121 ± 0.076
0.289HisCys: 0.289 ± 0.025
0.811HisAsp: 0.811 ± 0.044
0.875HisGlu: 0.875 ± 0.046
0.794HisPhe: 0.794 ± 0.04
1.785HisGly: 1.785 ± 0.062
0.699HisHis: 0.699 ± 0.044
1.312HisIle: 1.312 ± 0.058
0.501HisLys: 0.501 ± 0.035
3.214HisLeu: 3.214 ± 0.083
0.448HisMet: 0.448 ± 0.029
0.52HisAsn: 0.52 ± 0.032
2.221HisPro: 2.221 ± 0.064
0.917HisGln: 0.917 ± 0.051
2.062HisArg: 2.062 ± 0.07
0.864HisSer: 0.864 ± 0.039
1.359HisThr: 1.359 ± 0.053
0.928HisVal: 0.928 ± 0.044
0.37HisTrp: 0.37 ± 0.027
0.706HisTyr: 0.706 ± 0.041
0.0HisXaa: 0.0 ± 0.0
Ile
5.247IleAla: 5.247 ± 0.104
0.425IleCys: 0.425 ± 0.032
1.815IleAsp: 1.815 ± 0.065
2.1IleGlu: 2.1 ± 0.071
1.219IlePhe: 1.219 ± 0.055
3.991IleGly: 3.991 ± 0.095
0.9IleHis: 0.9 ± 0.045
1.764IleIle: 1.764 ± 0.069
0.919IleLys: 0.919 ± 0.048
4.693IleLeu: 4.693 ± 0.103
0.651IleMet: 0.651 ± 0.034
0.909IleAsn: 0.909 ± 0.043
3.235IlePro: 3.235 ± 0.085
1.376IleGln: 1.376 ± 0.066
3.153IleArg: 3.153 ± 0.088
1.792IleSer: 1.792 ± 0.059
2.544IleThr: 2.544 ± 0.07
3.501IleVal: 3.501 ± 0.094
0.465IleTrp: 0.465 ± 0.032
1.05IleTyr: 1.05 ± 0.056
0.0IleXaa: 0.0 ± 0.0
Lys
3.632LysAla: 3.632 ± 0.09
0.137LysCys: 0.137 ± 0.019
1.253LysAsp: 1.253 ± 0.049
2.219LysGlu: 2.219 ± 0.071
0.524LysPhe: 0.524 ± 0.032
3.106LysGly: 3.106 ± 0.079
0.406LysHis: 0.406 ± 0.028
0.906LysIle: 0.906 ± 0.052
0.682LysLys: 0.682 ± 0.043
1.559LysLeu: 1.559 ± 0.066
0.52LysMet: 0.52 ± 0.035
0.442LysAsn: 0.442 ± 0.028
1.707LysPro: 1.707 ± 0.06
0.71LysGln: 0.71 ± 0.045
2.143LysArg: 2.143 ± 0.084
0.925LysSer: 0.925 ± 0.045
1.325LysThr: 1.325 ± 0.051
2.916LysVal: 2.916 ± 0.076
0.304LysTrp: 0.304 ± 0.026
0.537LysTyr: 0.537 ± 0.036
0.0LysXaa: 0.0 ± 0.0
Leu
14.842LeuAla: 14.842 ± 0.218
1.158LeuCys: 1.158 ± 0.055
4.919LeuAsp: 4.919 ± 0.1
7.873LeuGlu: 7.873 ± 0.15
3.448LeuPhe: 3.448 ± 0.086
10.742LeuGly: 10.742 ± 0.192
2.487LeuHis: 2.487 ± 0.091
3.37LeuIle: 3.37 ± 0.088
3.066LeuLys: 3.066 ± 0.086
13.686LeuLeu: 13.686 ± 0.247
1.881LeuMet: 1.881 ± 0.063
1.946LeuAsn: 1.946 ± 0.064
7.372LeuPro: 7.372 ± 0.138
4.393LeuGln: 4.393 ± 0.101
9.663LeuArg: 9.663 ± 0.148
5.289LeuSer: 5.289 ± 0.115
5.733LeuThr: 5.733 ± 0.114
9.914LeuVal: 9.914 ± 0.172
1.684LeuTrp: 1.684 ± 0.065
2.472LeuTyr: 2.472 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
2.867MetAla: 2.867 ± 0.088
0.167MetCys: 0.167 ± 0.019
0.915MetAsp: 0.915 ± 0.048
1.433MetGlu: 1.433 ± 0.056
0.412MetPhe: 0.412 ± 0.029
2.341MetGly: 2.341 ± 0.08
0.404MetHis: 0.404 ± 0.031
0.399MetIle: 0.399 ± 0.032
0.49MetLys: 0.49 ± 0.031
1.891MetLeu: 1.891 ± 0.067
0.306MetMet: 0.306 ± 0.028
0.344MetAsn: 0.344 ± 0.025
1.454MetPro: 1.454 ± 0.053
0.583MetGln: 0.583 ± 0.038
1.752MetArg: 1.752 ± 0.064
0.852MetSer: 0.852 ± 0.036
0.987MetThr: 0.987 ± 0.046
1.783MetVal: 1.783 ± 0.071
0.209MetTrp: 0.209 ± 0.023
0.277MetTyr: 0.277 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.263AsnAla: 2.263 ± 0.074
0.209AsnCys: 0.209 ± 0.021
0.737AsnAsp: 0.737 ± 0.038
0.778AsnGlu: 0.778 ± 0.035
0.676AsnPhe: 0.676 ± 0.04
1.747AsnGly: 1.747 ± 0.069
0.33AsnHis: 0.33 ± 0.028
1.137AsnIle: 1.137 ± 0.053
0.427AsnLys: 0.427 ± 0.031
2.35AsnLeu: 2.35 ± 0.06
0.382AsnMet: 0.382 ± 0.029
0.454AsnAsn: 0.454 ± 0.03
2.014AsnPro: 2.014 ± 0.065
0.488AsnGln: 0.488 ± 0.032
1.401AsnArg: 1.401 ± 0.056
0.655AsnSer: 0.655 ± 0.036
1.073AsnThr: 1.073 ± 0.051
1.014AsnVal: 1.014 ± 0.049
0.27AsnTrp: 0.27 ± 0.023
0.461AsnTyr: 0.461 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
5.919ProAla: 5.919 ± 0.127
0.585ProCys: 0.585 ± 0.034
3.019ProAsp: 3.019 ± 0.086
4.139ProGlu: 4.139 ± 0.088
2.483ProPhe: 2.483 ± 0.072
5.745ProGly: 5.745 ± 0.138
1.756ProHis: 1.756 ± 0.065
2.498ProIle: 2.498 ± 0.081
1.578ProLys: 1.578 ± 0.059
7.643ProLeu: 7.643 ± 0.141
1.183ProMet: 1.183 ± 0.049
1.316ProAsn: 1.316 ± 0.058
5.566ProPro: 5.566 ± 0.16
3.028ProGln: 3.028 ± 0.091
4.773ProArg: 4.773 ± 0.104
3.82ProSer: 3.82 ± 0.092
4.205ProThr: 4.205 ± 0.187
5.008ProVal: 5.008 ± 0.106
1.154ProTrp: 1.154 ± 0.058
1.836ProTyr: 1.836 ± 0.067
0.0ProXaa: 0.0 ± 0.0
Gln
5.384GlnAla: 5.384 ± 0.12
0.262GlnCys: 0.262 ± 0.027
1.665GlnAsp: 1.665 ± 0.062
3.867GlnGlu: 3.867 ± 0.108
0.953GlnPhe: 0.953 ± 0.049
4.247GlnGly: 4.247 ± 0.095
0.816GlnHis: 0.816 ± 0.051
1.063GlnIle: 1.063 ± 0.048
1.232GlnLys: 1.232 ± 0.058
1.766GlnLeu: 1.766 ± 0.06
0.83GlnMet: 0.83 ± 0.042
0.604GlnAsn: 0.604 ± 0.04
1.912GlnPro: 1.912 ± 0.067
1.559GlnGln: 1.559 ± 0.071
4.125GlnArg: 4.125 ± 0.113
1.204GlnSer: 1.204 ± 0.049
1.669GlnThr: 1.669 ± 0.065
4.615GlnVal: 4.615 ± 0.1
0.668GlnTrp: 0.668 ± 0.043
0.606GlnTyr: 0.606 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
7.469ArgAla: 7.469 ± 0.136
0.841ArgCys: 0.841 ± 0.044
3.062ArgAsp: 3.062 ± 0.082
5.923ArgGlu: 5.923 ± 0.12
2.84ArgPhe: 2.84 ± 0.077
6.301ArgGly: 6.301 ± 0.116
1.826ArgHis: 1.826 ± 0.063
4.199ArgIle: 4.199 ± 0.084
2.119ArgLys: 2.119 ± 0.073
11.011ArgLeu: 11.011 ± 0.169
1.914ArgMet: 1.914 ± 0.059
1.496ArgAsn: 1.496 ± 0.055
5.456ArgPro: 5.456 ± 0.116
3.624ArgGln: 3.624 ± 0.107
8.07ArgArg: 8.07 ± 0.146
3.442ArgSer: 3.442 ± 0.081
3.584ArgThr: 3.584 ± 0.095
6.377ArgVal: 6.377 ± 0.131
2.009ArgTrp: 2.009 ± 0.067
2.312ArgTyr: 2.312 ± 0.071
0.0ArgXaa: 0.0 ± 0.0
Ser
5.79SerAla: 5.79 ± 0.122
0.446SerCys: 0.446 ± 0.031
1.469SerAsp: 1.469 ± 0.055
1.593SerGlu: 1.593 ± 0.058
1.464SerPhe: 1.464 ± 0.056
3.573SerGly: 3.573 ± 0.083
0.915SerHis: 0.915 ± 0.052
1.912SerIle: 1.912 ± 0.074
1.048SerLys: 1.048 ± 0.053
5.593SerLeu: 5.593 ± 0.127
0.887SerMet: 0.887 ± 0.043
0.778SerAsn: 0.778 ± 0.039
3.877SerPro: 3.877 ± 0.102
1.526SerGln: 1.526 ± 0.069
3.047SerArg: 3.047 ± 0.083
1.914SerSer: 1.914 ± 0.072
2.362SerThr: 2.362 ± 0.074
3.074SerVal: 3.074 ± 0.086
0.682SerTrp: 0.682 ± 0.039
1.012SerTyr: 1.012 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
5.411ThrAla: 5.411 ± 0.108
0.488ThrCys: 0.488 ± 0.033
1.916ThrAsp: 1.916 ± 0.069
2.024ThrGlu: 2.024 ± 0.066
1.984ThrPhe: 1.984 ± 0.072
4.676ThrGly: 4.676 ± 0.106
1.135ThrHis: 1.135 ± 0.048
2.075ThrIle: 2.075 ± 0.064
0.885ThrLys: 0.885 ± 0.048
6.582ThrLeu: 6.582 ± 0.13
0.735ThrMet: 0.735 ± 0.037
0.921ThrAsn: 0.921 ± 0.044
5.316ThrPro: 5.316 ± 0.193
1.581ThrGln: 1.581 ± 0.055
3.313ThrArg: 3.313 ± 0.082
2.13ThrSer: 2.13 ± 0.073
2.652ThrThr: 2.652 ± 0.079
4.437ThrVal: 4.437 ± 0.097
0.716ThrTrp: 0.716 ± 0.044
1.409ThrTyr: 1.409 ± 0.054
0.0ThrXaa: 0.0 ± 0.0
Val
9.563ValAla: 9.563 ± 0.151
0.934ValCys: 0.934 ± 0.046
4.023ValAsp: 4.023 ± 0.095
5.988ValGlu: 5.988 ± 0.111
2.984ValPhe: 2.984 ± 0.098
7.383ValGly: 7.383 ± 0.154
1.99ValHis: 1.99 ± 0.054
3.26ValIle: 3.26 ± 0.08
2.326ValLys: 2.326 ± 0.066
9.948ValLeu: 9.948 ± 0.179
1.532ValMet: 1.532 ± 0.061
1.817ValAsn: 1.817 ± 0.062
5.016ValPro: 5.016 ± 0.114
3.311ValGln: 3.311 ± 0.085
7.243ValArg: 7.243 ± 0.135
3.998ValSer: 3.998 ± 0.095
3.56ValThr: 3.56 ± 0.088
9.289ValVal: 9.289 ± 0.162
1.234ValTrp: 1.234 ± 0.053
1.91ValTyr: 1.91 ± 0.069
0.0ValXaa: 0.0 ± 0.0
Trp
1.731TrpAla: 1.731 ± 0.067
0.146TrpCys: 0.146 ± 0.017
0.672TrpAsp: 0.672 ± 0.042
1.295TrpGlu: 1.295 ± 0.051
0.463TrpPhe: 0.463 ± 0.03
1.57TrpGly: 1.57 ± 0.061
0.385TrpHis: 0.385 ± 0.026
0.393TrpIle: 0.393 ± 0.031
0.545TrpLys: 0.545 ± 0.033
1.853TrpLeu: 1.853 ± 0.068
0.359TrpMet: 0.359 ± 0.027
0.344TrpAsn: 0.344 ± 0.027
0.835TrpPro: 0.835 ± 0.036
0.628TrpGln: 0.628 ± 0.043
1.424TrpArg: 1.424 ± 0.054
0.632TrpSer: 0.632 ± 0.037
0.737TrpThr: 0.737 ± 0.042
1.45TrpVal: 1.45 ± 0.061
0.368TrpTrp: 0.368 ± 0.031
0.315TrpTyr: 0.315 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.517TyrAla: 2.517 ± 0.071
0.285TyrCys: 0.285 ± 0.026
1.152TyrAsp: 1.152 ± 0.047
1.304TyrGlu: 1.304 ± 0.058
0.917TyrPhe: 0.917 ± 0.051
2.282TyrGly: 2.282 ± 0.071
0.613TyrHis: 0.613 ± 0.04
1.168TyrIle: 1.168 ± 0.057
0.579TyrLys: 0.579 ± 0.033
3.087TyrLeu: 3.087 ± 0.088
0.389TyrMet: 0.389 ± 0.029
0.623TyrAsn: 0.623 ± 0.036
1.766TyrPro: 1.766 ± 0.067
1.147TyrGln: 1.147 ± 0.048
2.39TyrArg: 2.39 ± 0.081
1.042TyrSer: 1.042 ± 0.05
1.422TyrThr: 1.422 ± 0.055
1.371TyrVal: 1.371 ± 0.05
0.376TyrTrp: 0.376 ± 0.033
0.744TyrTyr: 0.744 ± 0.042
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1642 proteins (473262 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski