Xanthomonadaceae bacterium
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3169 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q6C7C1|A0A4Q6C7C1_9GAMM TerC family protein (Fragment) OS=Xanthomonadaceae bacterium OX=1926873 GN=EOP91_07615 PE=4 SV=1
MM1 pKa = 7.68 TDD3 pKa = 3.03 STDD6 pKa = 3.15 ARR8 pKa = 11.84 YY9 pKa = 7.35 QTWMCVVCGFIYY21 pKa = 10.67 DD22 pKa = 3.87 EE23 pKa = 4.55 AKK25 pKa = 10.57 GLPEE29 pKa = 4.53 EE30 pKa = 4.68 GLAPGTRR37 pKa = 11.84 WKK39 pKa = 10.41 DD40 pKa = 3.32 IPDD43 pKa = 3.11 SWTCPDD49 pKa = 4.16 CGVGKK54 pKa = 10.25 DD55 pKa = 3.81 DD56 pKa = 4.93 FEE58 pKa = 4.83 MIEE61 pKa = 3.83 VDD63 pKa = 3.41
Molecular weight: 7.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.872
IPC_protein 3.795
Toseland 3.592
ProMoST 3.897
Dawson 3.808
Bjellqvist 4.024
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.923
Patrickios 1.901
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.87
Protein with the highest isoelectric point:
>tr|A0A4Q6C685|A0A4Q6C685_9GAMM Fused isobutyryl-CoA mutase OS=Xanthomonadaceae bacterium OX=1926873 GN=icmF PE=3 SV=1
MM1 pKa = 7.91 LDD3 pKa = 3.7 RR4 pKa = 11.84 VRR6 pKa = 11.84 DD7 pKa = 3.88 CCRR10 pKa = 11.84 VRR12 pKa = 11.84 HH13 pKa = 5.04 YY14 pKa = 10.96 SLRR17 pKa = 11.84 TEE19 pKa = 3.94 RR20 pKa = 11.84 AYY22 pKa = 11.33 VDD24 pKa = 3.71 WIRR27 pKa = 11.84 RR28 pKa = 11.84 FVVANDD34 pKa = 3.09 KK35 pKa = 10.53 RR36 pKa = 11.84 HH37 pKa = 5.77 PRR39 pKa = 11.84 TMGAPEE45 pKa = 3.97 VEE47 pKa = 4.55 AFLSTLATTHH57 pKa = 7.0 DD58 pKa = 4.12 VAASTQNQALSALLFLYY75 pKa = 10.6 RR76 pKa = 11.84 EE77 pKa = 4.25 VLGMQLPWMEE87 pKa = 4.0 SVVRR91 pKa = 11.84 AKK93 pKa = 10.44 RR94 pKa = 11.84 PQKK97 pKa = 10.32 IPAVLSRR104 pKa = 11.84 DD105 pKa = 3.35 EE106 pKa = 4.22 VGRR109 pKa = 11.84 LLALMDD115 pKa = 3.85 GVRR118 pKa = 11.84 NGKK121 pKa = 9.27 GGKK124 pKa = 8.65 DD125 pKa = 2.79 RR126 pKa = 11.84 RR127 pKa = 11.84 VPLPRR132 pKa = 11.84 RR133 pKa = 11.84 LRR135 pKa = 11.84 EE136 pKa = 3.74 RR137 pKa = 11.84 LLEE140 pKa = 3.8 QVEE143 pKa = 4.43 RR144 pKa = 11.84 VRR146 pKa = 11.84 VQHH149 pKa = 6.38 GRR151 pKa = 11.84 DD152 pKa = 3.23 LAGGAGEE159 pKa = 4.46 VYY161 pKa = 10.39 LPHH164 pKa = 7.06 ALARR168 pKa = 11.84 KK169 pKa = 8.69 YY170 pKa = 10.56 PNAGRR175 pKa = 11.84 QFCWQYY181 pKa = 10.63 VFPSPRR187 pKa = 11.84 EE188 pKa = 3.84 SRR190 pKa = 11.84 DD191 pKa = 3.31 PRR193 pKa = 11.84 SGRR196 pKa = 11.84 VRR198 pKa = 11.84 RR199 pKa = 11.84 HH200 pKa = 6.21 HH201 pKa = 6.72 IDD203 pKa = 3.06 EE204 pKa = 4.85 GVLQRR209 pKa = 11.84 AVKK212 pKa = 9.77 VARR215 pKa = 11.84 DD216 pKa = 3.11 RR217 pKa = 11.84 AGIDD221 pKa = 3.43 KK222 pKa = 9.86 PASCHH227 pKa = 5.08 TLRR230 pKa = 11.84 HH231 pKa = 5.5 SFATHH236 pKa = 6.51 LLEE239 pKa = 4.74 SGQDD243 pKa = 2.86 IRR245 pKa = 11.84 TVQEE249 pKa = 3.9 LMGHH253 pKa = 6.42 KK254 pKa = 10.07 DD255 pKa = 3.11 VSTTQIYY262 pKa = 7.47 THH264 pKa = 5.38 VLGRR268 pKa = 11.84 GAHH271 pKa = 5.8 GVLSPLDD278 pKa = 3.41 QQ279 pKa = 4.9
Molecular weight: 31.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.56
IPC_protein 10.555
Toseland 10.584
ProMoST 10.409
Dawson 10.701
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.745
Grimsley 10.76
Solomon 10.862
Lehninger 10.804
Nozaki 10.599
DTASelect 10.467
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.643
Patrickios 10.423
IPC_peptide 10.862
IPC2_peptide 9.648
IPC2.peptide.svr19 8.626
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3169
0
3169
897507
19
1915
283.2
30.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.033 ± 0.066
0.834 ± 0.014
5.666 ± 0.032
5.422 ± 0.043
3.411 ± 0.03
8.808 ± 0.044
2.199 ± 0.023
4.034 ± 0.035
2.76 ± 0.036
10.988 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.181 ± 0.018
2.442 ± 0.03
5.467 ± 0.039
3.805 ± 0.029
7.673 ± 0.051
5.413 ± 0.032
4.588 ± 0.038
7.413 ± 0.034
1.544 ± 0.021
2.319 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here