Amino acid dipepetide frequency for Amoebophilus asiaticus (strain 5a2)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.87AlaAla: 5.87 ± 0.167	0.84AlaCys: 0.84 ± 0.057	3.532AlaAsp: 3.532 ± 0.11	4.323AlaGlu: 4.323 ± 0.131	2.644AlaPhe: 2.644 ± 0.069	4.053AlaGly: 4.053 ± 0.106	1.622AlaHis: 1.622 ± 0.067	6.504AlaIle: 6.504 ± 0.137	5.815AlaLys: 5.815 ± 0.152	7.405AlaLeu: 7.405 ± 0.188	1.409AlaMet: 1.409 ± 0.064	3.362AlaAsn: 3.362 ± 0.095	1.915AlaPro: 1.915 ± 0.067	3.709AlaGln: 3.709 ± 0.154	2.837AlaArg: 2.837 ± 0.085	4.606AlaSer: 4.606 ± 0.106	4.081AlaThr: 4.081 ± 0.104	3.951AlaVal: 3.951 ± 0.119	0.697AlaTrp: 0.697 ± 0.044	2.937AlaTyr: 2.937 ± 0.098	0.0AlaXaa: 0.0 ± 0.0
Cys	0.502CysAla: 0.502 ± 0.033	0.098CysCys: 0.098 ± 0.015	0.391CysAsp: 0.391 ± 0.028	0.453CysGlu: 0.453 ± 0.034	0.468CysPhe: 0.468 ± 0.027	0.646CysGly: 0.646 ± 0.032	0.206CysHis: 0.206 ± 0.022	0.844CysIle: 0.844 ± 0.046	0.718CysLys: 0.718 ± 0.045	1.056CysLeu: 1.056 ± 0.048	0.259CysMet: 0.259 ± 0.025	0.516CysAsn: 0.516 ± 0.035	0.366CysPro: 0.366 ± 0.027	0.446CysGln: 0.446 ± 0.031	0.438CysArg: 0.438 ± 0.03	0.584CysSer: 0.584 ± 0.037	0.465CysThr: 0.465 ± 0.035	0.457CysVal: 0.457 ± 0.032	0.091CysTrp: 0.091 ± 0.013	0.455CysTyr: 0.455 ± 0.033	0.0CysXaa: 0.0 ± 0.0
Asp	2.986AspAla: 2.986 ± 0.097	0.417AspCys: 0.417 ± 0.031	1.702AspAsp: 1.702 ± 0.086	2.988AspGlu: 2.988 ± 0.091	2.132AspPhe: 2.132 ± 0.071	2.176AspGly: 2.176 ± 0.077	0.891AspHis: 0.891 ± 0.046	4.699AspIle: 4.699 ± 0.113	4.568AspLys: 4.568 ± 0.13	4.463AspLeu: 4.463 ± 0.107	0.946AspMet: 0.946 ± 0.047	2.633AspAsn: 2.633 ± 0.087	1.539AspPro: 1.539 ± 0.061	1.828AspGln: 1.828 ± 0.073	1.907AspArg: 1.907 ± 0.066	2.495AspSer: 2.495 ± 0.081	2.778AspThr: 2.778 ± 0.09	2.468AspVal: 2.468 ± 0.077	0.478AspTrp: 0.478 ± 0.032	1.819AspTyr: 1.819 ± 0.07	0.0AspXaa: 0.0 ± 0.0
Glu	5.265GluAla: 5.265 ± 0.162	0.397GluCys: 0.397 ± 0.028	3.331GluAsp: 3.331 ± 0.107	6.157GluGlu: 6.157 ± 0.203	2.023GluPhe: 2.023 ± 0.077	3.656GluGly: 3.656 ± 0.09	1.441GluHis: 1.441 ± 0.049	5.224GluIle: 5.224 ± 0.128	6.423GluLys: 6.423 ± 0.177	6.825GluLeu: 6.825 ± 0.149	1.212GluMet: 1.212 ± 0.049	3.271GluAsn: 3.271 ± 0.092	1.702GluPro: 1.702 ± 0.059	3.917GluGln: 3.917 ± 0.117	3.014GluArg: 3.014 ± 0.11	3.097GluSer: 3.097 ± 0.078	2.759GluThr: 2.759 ± 0.078	4.055GluVal: 4.055 ± 0.106	0.714GluTrp: 0.714 ± 0.069	2.032GluTyr: 2.032 ± 0.069	0.0GluXaa: 0.0 ± 0.0
Phe	2.174PheAla: 2.174 ± 0.078	0.453PheCys: 0.453 ± 0.03	1.811PheAsp: 1.811 ± 0.066	2.179PheGlu: 2.179 ± 0.074	1.792PhePhe: 1.792 ± 0.068	2.0PheGly: 2.0 ± 0.085	0.75PheHis: 0.75 ± 0.037	3.479PheIle: 3.479 ± 0.108	3.175PheLys: 3.175 ± 0.089	3.958PheLeu: 3.958 ± 0.108	0.886PheMet: 0.886 ± 0.047	2.155PheAsn: 2.155 ± 0.078	1.309PhePro: 1.309 ± 0.069	1.679PheGln: 1.679 ± 0.065	1.399PheArg: 1.399 ± 0.056	2.969PheSer: 2.969 ± 0.086	2.363PheThr: 2.363 ± 0.087	1.966PheVal: 1.966 ± 0.073	0.417PheTrp: 0.417 ± 0.033	1.928PheTyr: 1.928 ± 0.069	0.0PheXaa: 0.0 ± 0.0
Gly	3.987GlyAla: 3.987 ± 0.148	0.693GlyCys: 0.693 ± 0.04	2.502GlyAsp: 2.502 ± 0.08	3.382GlyGlu: 3.382 ± 0.092	2.397GlyPhe: 2.397 ± 0.086	3.528GlyGly: 3.528 ± 0.106	1.594GlyHis: 1.594 ± 0.077	5.399GlyIle: 5.399 ± 0.14	5.118GlyLys: 5.118 ± 0.119	5.747GlyLeu: 5.747 ± 0.136	1.42GlyMet: 1.42 ± 0.06	3.333GlyAsn: 3.333 ± 0.115	1.252GlyPro: 1.252 ± 0.058	2.298GlyGln: 2.298 ± 0.084	2.321GlyArg: 2.321 ± 0.083	3.029GlySer: 3.029 ± 0.077	3.256GlyThr: 3.256 ± 0.1	3.462GlyVal: 3.462 ± 0.13	0.765GlyTrp: 0.765 ± 0.049	2.893GlyTyr: 2.893 ± 0.082	0.0GlyXaa: 0.0 ± 0.0
His	1.594HisAla: 1.594 ± 0.067	0.236HisCys: 0.236 ± 0.027	0.85HisAsp: 0.85 ± 0.044	1.131HisGlu: 1.131 ± 0.046	1.029HisPhe: 1.029 ± 0.054	1.216HisGly: 1.216 ± 0.047	0.578HisHis: 0.578 ± 0.045	2.538HisIle: 2.538 ± 0.071	1.715HisLys: 1.715 ± 0.06	2.665HisLeu: 2.665 ± 0.102	0.508HisMet: 0.508 ± 0.034	1.239HisAsn: 1.239 ± 0.054	1.186HisPro: 1.186 ± 0.06	1.197HisGln: 1.197 ± 0.068	0.797HisArg: 0.797 ± 0.048	1.22HisSer: 1.22 ± 0.057	1.739HisThr: 1.739 ± 0.08	1.226HisVal: 1.226 ± 0.053	0.283HisTrp: 0.283 ± 0.031	1.075HisTyr: 1.075 ± 0.049	0.0HisXaa: 0.0 ± 0.0
Ile	6.206IleAla: 6.206 ± 0.143	0.776IleCys: 0.776 ± 0.042	4.616IleAsp: 4.616 ± 0.111	5.813IleGlu: 5.813 ± 0.131	3.001IlePhe: 3.001 ± 0.1	5.235IleGly: 5.235 ± 0.149	1.885IleHis: 1.885 ± 0.06	5.972IleIle: 5.972 ± 0.146	6.336IleLys: 6.336 ± 0.139	7.724IleLeu: 7.724 ± 0.162	1.326IleMet: 1.326 ± 0.054	4.393IleAsn: 4.393 ± 0.127	3.307IlePro: 3.307 ± 0.081	4.196IleGln: 4.196 ± 0.082	3.118IleArg: 3.118 ± 0.085	5.405IleSer: 5.405 ± 0.115	4.812IleThr: 4.812 ± 0.129	4.733IleVal: 4.733 ± 0.105	0.657IleTrp: 0.657 ± 0.04	2.986IleTyr: 2.986 ± 0.103	0.0IleXaa: 0.0 ± 0.0
Lys	6.109LysAla: 6.109 ± 0.206	0.461LysCys: 0.461 ± 0.028	4.417LysAsp: 4.417 ± 0.12	6.597LysGlu: 6.597 ± 0.154	2.217LysPhe: 2.217 ± 0.064	5.131LysGly: 5.131 ± 0.153	2.004LysHis: 2.004 ± 0.072	5.403LysIle: 5.403 ± 0.117	6.527LysLys: 6.527 ± 0.168	8.194LysLeu: 8.194 ± 0.166	1.405LysMet: 1.405 ± 0.06	4.249LysAsn: 4.249 ± 0.119	2.58LysPro: 2.58 ± 0.075	4.21LysGln: 4.21 ± 0.097	3.737LysArg: 3.737 ± 0.105	4.489LysSer: 4.489 ± 0.112	3.639LysThr: 3.639 ± 0.086	4.429LysVal: 4.429 ± 0.109	0.803LysTrp: 0.803 ± 0.046	2.993LysTyr: 2.993 ± 0.107	0.0LysXaa: 0.0 ± 0.0
Leu	8.5LeuAla: 8.5 ± 0.149	1.075LeuCys: 1.075 ± 0.047	4.833LeuAsp: 4.833 ± 0.121	7.173LeuGlu: 7.173 ± 0.164	4.04LeuPhe: 4.04 ± 0.112	6.68LeuGly: 6.68 ± 0.174	2.982LeuHis: 2.982 ± 0.146	7.696LeuIle: 7.696 ± 0.174	7.564LeuLys: 7.564 ± 0.155	11.902LeuLeu: 11.902 ± 0.271	1.968LeuMet: 1.968 ± 0.071	4.51LeuAsn: 4.51 ± 0.112	4.506LeuPro: 4.506 ± 0.107	5.675LeuGln: 5.675 ± 0.145	3.73LeuArg: 3.73 ± 0.095	6.986LeuSer: 6.986 ± 0.128	5.577LeuThr: 5.577 ± 0.114	5.796LeuVal: 5.796 ± 0.116	0.803LeuTrp: 0.803 ± 0.045	3.93LeuTyr: 3.93 ± 0.098	0.0LeuXaa: 0.0 ± 0.0
Met	1.488MetAla: 1.488 ± 0.06	0.179MetCys: 0.179 ± 0.019	0.954MetAsp: 0.954 ± 0.046	1.19MetGlu: 1.19 ± 0.051	0.614MetPhe: 0.614 ± 0.043	1.218MetGly: 1.218 ± 0.054	0.536MetHis: 0.536 ± 0.032	1.292MetIle: 1.292 ± 0.055	1.252MetLys: 1.252 ± 0.051	2.366MetLeu: 2.366 ± 0.069	0.344MetMet: 0.344 ± 0.025	0.721MetAsn: 0.721 ± 0.035	0.918MetPro: 0.918 ± 0.042	1.173MetGln: 1.173 ± 0.05	1.003MetArg: 1.003 ± 0.055	1.112MetSer: 1.112 ± 0.055	0.661MetThr: 0.661 ± 0.038	1.18MetVal: 1.18 ± 0.059	0.153MetTrp: 0.153 ± 0.018	0.952MetTyr: 0.952 ± 0.082	0.0MetXaa: 0.0 ± 0.0
Asn	3.173AsnAla: 3.173 ± 0.105	0.478AsnCys: 0.478 ± 0.038	1.804AsnAsp: 1.804 ± 0.078	2.582AsnGlu: 2.582 ± 0.09	2.111AsnPhe: 2.111 ± 0.07	2.349AsnGly: 2.349 ± 0.079	1.184AsnHis: 1.184 ± 0.068	4.442AsnIle: 4.442 ± 0.105	4.368AsnLys: 4.368 ± 0.113	5.894AsnLeu: 5.894 ± 0.128	0.937AsnMet: 0.937 ± 0.044	3.207AsnAsn: 3.207 ± 0.099	2.066AsnPro: 2.066 ± 0.069	2.672AsnGln: 2.672 ± 0.1	2.07AsnArg: 2.07 ± 0.066	2.99AsnSer: 2.99 ± 0.074	3.241AsnThr: 3.241 ± 0.111	2.459AsnVal: 2.459 ± 0.07	0.553AsnTrp: 0.553 ± 0.038	2.268AsnTyr: 2.268 ± 0.077	0.0AsnXaa: 0.0 ± 0.0
Pro	2.383ProAla: 2.383 ± 0.08	0.285ProCys: 0.285 ± 0.024	1.524ProAsp: 1.524 ± 0.065	2.1ProGlu: 2.1 ± 0.078	1.554ProPhe: 1.554 ± 0.074	1.928ProGly: 1.928 ± 0.07	0.984ProHis: 0.984 ± 0.043	3.452ProIle: 3.452 ± 0.089	2.276ProLys: 2.276 ± 0.068	4.057ProLeu: 4.057 ± 0.131	0.591ProMet: 0.591 ± 0.032	1.856ProAsn: 1.856 ± 0.076	0.929ProPro: 0.929 ± 0.05	1.428ProGln: 1.428 ± 0.063	1.071ProArg: 1.071 ± 0.046	2.453ProSer: 2.453 ± 0.08	2.504ProThr: 2.504 ± 0.074	2.172ProVal: 2.172 ± 0.075	0.361ProTrp: 0.361 ± 0.027	1.524ProTyr: 1.524 ± 0.064	0.0ProXaa: 0.0 ± 0.0
Gln	4.78GlnAla: 4.78 ± 0.144	0.293GlnCys: 0.293 ± 0.025	2.21GlnAsp: 2.21 ± 0.076	4.357GlnGlu: 4.357 ± 0.124	1.583GlnPhe: 1.583 ± 0.059	3.683GlnGly: 3.683 ± 0.151	1.243GlnHis: 1.243 ± 0.057	3.161GlnIle: 3.161 ± 0.095	3.615GlnLys: 3.615 ± 0.1	5.552GlnLeu: 5.552 ± 0.126	0.765GlnMet: 0.765 ± 0.04	2.004GlnAsn: 2.004 ± 0.077	1.76GlnPro: 1.76 ± 0.064	3.284GlnGln: 3.284 ± 0.118	1.938GlnArg: 1.938 ± 0.072	2.306GlnSer: 2.306 ± 0.074	2.174GlnThr: 2.174 ± 0.079	3.135GlnVal: 3.135 ± 0.098	0.446GlnTrp: 0.446 ± 0.046	1.777GlnTyr: 1.777 ± 0.082	0.0GlnXaa: 0.0 ± 0.0
Arg	2.527ArgAla: 2.527 ± 0.076	0.302ArgCys: 0.302 ± 0.021	1.702ArgAsp: 1.702 ± 0.061	2.595ArgGlu: 2.595 ± 0.079	1.728ArgPhe: 1.728 ± 0.071	2.155ArgGly: 2.155 ± 0.08	0.812ArgHis: 0.812 ± 0.043	3.522ArgIle: 3.522 ± 0.088	3.486ArgLys: 3.486 ± 0.09	4.27ArgLeu: 4.27 ± 0.103	1.012ArgMet: 1.012 ± 0.049	1.987ArgAsn: 1.987 ± 0.057	1.367ArgPro: 1.367 ± 0.061	1.739ArgGln: 1.739 ± 0.067	1.892ArgArg: 1.892 ± 0.075	2.03ArgSer: 2.03 ± 0.066	2.064ArgThr: 2.064 ± 0.075	2.193ArgVal: 2.193 ± 0.065	0.412ArgTrp: 0.412 ± 0.026	1.7ArgTyr: 1.7 ± 0.057	0.0ArgXaa: 0.0 ± 0.0
Ser	3.269SerAla: 3.269 ± 0.091	0.846SerCys: 0.846 ± 0.045	2.272SerAsp: 2.272 ± 0.07	3.169SerGlu: 3.169 ± 0.085	2.916SerPhe: 2.916 ± 0.084	3.141SerGly: 3.141 ± 0.101	1.433SerHis: 1.433 ± 0.068	5.66SerIle: 5.66 ± 0.103	4.536SerLys: 4.536 ± 0.097	7.148SerLeu: 7.148 ± 0.146	1.326SerMet: 1.326 ± 0.057	3.133SerAsn: 3.133 ± 0.082	2.006SerPro: 2.006 ± 0.066	2.621SerGln: 2.621 ± 0.079	2.123SerArg: 2.123 ± 0.079	4.353SerSer: 4.353 ± 0.187	3.598SerThr: 3.598 ± 0.097	3.141SerVal: 3.141 ± 0.074	0.616SerTrp: 0.616 ± 0.043	2.612SerTyr: 2.612 ± 0.091	0.0SerXaa: 0.0 ± 0.0
Thr	3.856ThrAla: 3.856 ± 0.108	0.485ThrCys: 0.485 ± 0.033	2.495ThrAsp: 2.495 ± 0.079	3.112ThrGlu: 3.112 ± 0.089	2.261ThrPhe: 2.261 ± 0.076	3.097ThrGly: 3.097 ± 0.087	1.365ThrHis: 1.365 ± 0.06	4.742ThrIle: 4.742 ± 0.124	3.783ThrLys: 3.783 ± 0.09	6.004ThrLeu: 6.004 ± 0.109	0.793ThrMet: 0.793 ± 0.046	2.797ThrAsn: 2.797 ± 0.085	2.85ThrPro: 2.85 ± 0.123	2.474ThrGln: 2.474 ± 0.079	1.862ThrArg: 1.862 ± 0.071	3.613ThrSer: 3.613 ± 0.102	3.473ThrThr: 3.473 ± 0.105	2.942ThrVal: 2.942 ± 0.094	0.572ThrTrp: 0.572 ± 0.035	2.438ThrTyr: 2.438 ± 0.083	0.0ThrXaa: 0.0 ± 0.0
Val	4.325ValAla: 4.325 ± 0.107	0.614ValCys: 0.614 ± 0.036	2.959ValAsp: 2.959 ± 0.082	4.043ValGlu: 4.043 ± 0.097	2.083ValPhe: 2.083 ± 0.073	3.539ValGly: 3.539 ± 0.105	1.339ValHis: 1.339 ± 0.076	4.253ValIle: 4.253 ± 0.112	4.391ValLys: 4.391 ± 0.142	5.343ValLeu: 5.343 ± 0.116	1.133ValMet: 1.133 ± 0.053	2.899ValAsn: 2.899 ± 0.09	2.053ValPro: 2.053 ± 0.065	2.38ValGln: 2.38 ± 0.077	2.047ValArg: 2.047 ± 0.068	3.458ValSer: 3.458 ± 0.08	2.837ValThr: 2.837 ± 0.088	3.418ValVal: 3.418 ± 0.107	0.436ValTrp: 0.436 ± 0.033	2.238ValTyr: 2.238 ± 0.075	0.0ValXaa: 0.0 ± 0.0
Trp	0.578TrpAla: 0.578 ± 0.037	0.091TrpCys: 0.091 ± 0.014	0.38TrpAsp: 0.38 ± 0.03	0.599TrpGlu: 0.599 ± 0.04	0.451TrpPhe: 0.451 ± 0.043	0.601TrpGly: 0.601 ± 0.035	0.249TrpHis: 0.249 ± 0.026	0.725TrpIle: 0.725 ± 0.041	0.74TrpLys: 0.74 ± 0.041	1.101TrpLeu: 1.101 ± 0.055	0.268TrpMet: 0.268 ± 0.026	0.459TrpAsn: 0.459 ± 0.033	0.332TrpPro: 0.332 ± 0.031	0.584TrpGln: 0.584 ± 0.038	0.4TrpArg: 0.4 ± 0.027	0.538TrpSer: 0.538 ± 0.038	0.361TrpThr: 0.361 ± 0.034	0.553TrpVal: 0.553 ± 0.037	0.104TrpTrp: 0.104 ± 0.015	0.67TrpTyr: 0.67 ± 0.071	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.542TyrAla: 2.542 ± 0.075	0.442TyrCys: 0.442 ± 0.03	1.626TyrAsp: 1.626 ± 0.066	2.572TyrGlu: 2.572 ± 0.095	1.798TyrPhe: 1.798 ± 0.067	2.342TyrGly: 2.342 ± 0.083	0.925TyrHis: 0.925 ± 0.05	3.492TyrIle: 3.492 ± 0.092	3.188TyrLys: 3.188 ± 0.082	4.255TyrLeu: 4.255 ± 0.119	0.795TyrMet: 0.795 ± 0.043	2.123TyrAsn: 2.123 ± 0.083	1.488TyrPro: 1.488 ± 0.063	2.559TyrGln: 2.559 ± 0.12	1.792TyrArg: 1.792 ± 0.068	2.187TyrSer: 2.187 ± 0.077	2.629TyrThr: 2.629 ± 0.074	2.051TyrVal: 2.051 ± 0.059	0.421TyrTrp: 0.421 ± 0.035	1.853TyrTyr: 1.853 ± 0.079	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1253 proteins (470492 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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