Vibrio phage CP-T1 (Bacteriophage CP-T1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9C0F0|H9C0F0_BPCPT Uncharacterized protein OS=Vibrio phage CP-T1 OX=10689 GN=CP-T1_0046 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 6.55LLIDD6 pKa = 3.88WLGDD10 pKa = 3.64CPNCGKK16 pKa = 10.05NDD18 pKa = 4.01PLQVTTTKK26 pKa = 10.88GSATLLYY33 pKa = 10.79VGDD36 pKa = 4.62EE37 pKa = 4.45VTCTYY42 pKa = 9.51CQHH45 pKa = 6.18GGEE48 pKa = 4.75IEE50 pKa = 4.17VCDD53 pKa = 3.86DD54 pKa = 3.35CTYY57 pKa = 11.33VNWGDD62 pKa = 4.33LPEE65 pKa = 5.58PDD67 pKa = 5.27DD68 pKa = 4.15GHH70 pKa = 7.16DD71 pKa = 3.29SAMM74 pKa = 4.01

Molecular weight: 8.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9C0B1|H9C0B1_BPCPT Uncharacterized protein OS=Vibrio phage CP-T1 OX=10689 GN=CP-T1_0007 PE=4 SV=1
MM1 pKa = 7.09FQRR4 pKa = 11.84IVALLMAQANHH15 pKa = 5.95TAANSIGNQLGRR27 pKa = 11.84PVSDD31 pKa = 3.62NAWGNRR37 pKa = 11.84KK38 pKa = 9.46GGRR41 pKa = 11.84KK42 pKa = 7.17QAHH45 pKa = 6.34RR46 pKa = 11.84FSGVAAQRR54 pKa = 11.84RR55 pKa = 11.84AARR58 pKa = 11.84KK59 pKa = 8.91IRR61 pKa = 11.84NQRR64 pKa = 11.84KK65 pKa = 9.16SKK67 pKa = 10.81

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13968

38

993

199.5

22.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.517 ± 0.342

1.468 ± 0.158

6.901 ± 0.217

5.484 ± 0.205

3.537 ± 0.223

7.36 ± 0.285

1.933 ± 0.173

6.823 ± 0.261

5.262 ± 0.332

7.66 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.144

5.176 ± 0.202

4.396 ± 0.313

3.723 ± 0.223

5.491 ± 0.274

6.128 ± 0.188

6.708 ± 0.286

6.959 ± 0.254

1.425 ± 0.121

3.451 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski