Catonella morbi ATCC 51271
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3288 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V2Y2B9|V2Y2B9_9FIRM Putative transposase OS=Catonella morbi ATCC 51271 OX=592026 GN=GCWU0000282_002336 PE=4 SV=1
MM1 pKa = 7.45 SEE3 pKa = 3.74 QRR5 pKa = 11.84 ITKK8 pKa = 10.36 DD9 pKa = 3.18 MIIADD14 pKa = 4.5 IIQIDD19 pKa = 4.07 EE20 pKa = 4.12 NLIPVLLNAGMHH32 pKa = 6.09 CVGCPSSMGEE42 pKa = 4.15 TLEE45 pKa = 4.21 EE46 pKa = 3.85 AAEE49 pKa = 4.09 VHH51 pKa = 7.15 GIDD54 pKa = 5.3 ADD56 pKa = 4.14 EE57 pKa = 5.12 LCDD60 pKa = 4.81 LLNEE64 pKa = 5.37 FIDD67 pKa = 3.72
Molecular weight: 7.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.795
IPC_protein 3.694
Toseland 3.516
ProMoST 3.783
Dawson 3.694
Bjellqvist 3.923
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.439
Solomon 3.656
Lehninger 3.617
Nozaki 3.821
DTASelect 3.973
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.821
Patrickios 1.85
IPC_peptide 3.656
IPC2_peptide 3.795
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|V2Y387|V2Y387_9FIRM Uncharacterized protein OS=Catonella morbi ATCC 51271 OX=592026 GN=GCWU0000282_002663 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 8.95 KK9 pKa = 7.58 RR10 pKa = 11.84 QRR12 pKa = 11.84 SKK14 pKa = 9.07 VHH16 pKa = 6.0 GFRR19 pKa = 11.84 KK20 pKa = 9.75 RR21 pKa = 11.84 MKK23 pKa = 8.33 TADD26 pKa = 3.28 GRR28 pKa = 11.84 RR29 pKa = 11.84 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.17 GRR39 pKa = 11.84 KK40 pKa = 8.79 KK41 pKa = 10.63 LSAA44 pKa = 3.95
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.407
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.427
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.149
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.037
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3288
0
3288
1036118
37
2273
315.1
35.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.72 ± 0.046
1.153 ± 0.018
5.653 ± 0.035
7.788 ± 0.047
4.508 ± 0.036
6.819 ± 0.044
1.389 ± 0.017
8.3 ± 0.04
8.585 ± 0.044
8.886 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.824 ± 0.023
5.271 ± 0.033
2.931 ± 0.02
2.252 ± 0.02
3.889 ± 0.031
6.134 ± 0.03
5.219 ± 0.038
6.636 ± 0.039
0.822 ± 0.014
4.222 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here