Amino acid dipepetide frequency for Betaproteobacteria bacterium UKL13-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.368AlaAla: 14.368 ± 0.184
1.051AlaCys: 1.051 ± 0.039
5.978AlaAsp: 5.978 ± 0.101
6.369AlaGlu: 6.369 ± 0.098
4.098AlaPhe: 4.098 ± 0.08
8.561AlaGly: 8.561 ± 0.118
2.079AlaHis: 2.079 ± 0.054
6.464AlaIle: 6.464 ± 0.096
5.656AlaLys: 5.656 ± 0.091
11.591AlaLeu: 11.591 ± 0.147
3.366AlaMet: 3.366 ± 0.065
3.906AlaAsn: 3.906 ± 0.074
4.881AlaPro: 4.881 ± 0.095
4.091AlaGln: 4.091 ± 0.072
6.42AlaArg: 6.42 ± 0.112
6.827AlaSer: 6.827 ± 0.089
6.86AlaThr: 6.86 ± 0.148
8.069AlaVal: 8.069 ± 0.133
1.375AlaTrp: 1.375 ± 0.039
2.447AlaTyr: 2.447 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
1.062CysAla: 1.062 ± 0.045
0.114CysCys: 0.114 ± 0.012
0.545CysAsp: 0.545 ± 0.027
0.556CysGlu: 0.556 ± 0.029
0.346CysPhe: 0.346 ± 0.023
0.944CysGly: 0.944 ± 0.039
0.252CysHis: 0.252 ± 0.016
0.455CysIle: 0.455 ± 0.022
0.303CysLys: 0.303 ± 0.02
0.797CysLeu: 0.797 ± 0.032
0.203CysMet: 0.203 ± 0.014
0.265CysAsn: 0.265 ± 0.019
0.485CysPro: 0.485 ± 0.026
0.289CysGln: 0.289 ± 0.016
0.571CysArg: 0.571 ± 0.028
0.48CysSer: 0.48 ± 0.025
0.606CysThr: 0.606 ± 0.061
0.716CysVal: 0.716 ± 0.03
0.133CysTrp: 0.133 ± 0.018
0.225CysTyr: 0.225 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
6.375AspAla: 6.375 ± 0.111
0.562AspCys: 0.562 ± 0.024
2.846AspAsp: 2.846 ± 0.065
3.247AspGlu: 3.247 ± 0.06
2.282AspPhe: 2.282 ± 0.059
4.257AspGly: 4.257 ± 0.083
1.109AspHis: 1.109 ± 0.039
3.119AspIle: 3.119 ± 0.057
2.345AspLys: 2.345 ± 0.052
4.852AspLeu: 4.852 ± 0.084
1.173AspMet: 1.173 ± 0.035
1.387AspAsn: 1.387 ± 0.041
2.514AspPro: 2.514 ± 0.059
1.809AspGln: 1.809 ± 0.043
3.161AspArg: 3.161 ± 0.074
2.431AspSer: 2.431 ± 0.052
2.788AspThr: 2.788 ± 0.063
4.345AspVal: 4.345 ± 0.071
0.935AspTrp: 0.935 ± 0.03
1.559AspTyr: 1.559 ± 0.044
0.0AspXaa: 0.0 ± 0.0
Glu
6.086GluAla: 6.086 ± 0.092
0.382GluCys: 0.382 ± 0.021
2.27GluAsp: 2.27 ± 0.063
2.481GluGlu: 2.481 ± 0.058
2.064GluPhe: 2.064 ± 0.053
3.365GluGly: 3.365 ± 0.062
1.172GluHis: 1.172 ± 0.04
3.502GluIle: 3.502 ± 0.065
2.887GluLys: 2.887 ± 0.06
5.066GluLeu: 5.066 ± 0.074
1.501GluMet: 1.501 ± 0.04
1.767GluAsn: 1.767 ± 0.05
2.072GluPro: 2.072 ± 0.05
2.178GluGln: 2.178 ± 0.054
4.007GluArg: 4.007 ± 0.084
3.035GluSer: 3.035 ± 0.062
2.966GluThr: 2.966 ± 0.063
4.02GluVal: 4.02 ± 0.064
0.748GluTrp: 0.748 ± 0.033
1.268GluTyr: 1.268 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
4.523PheAla: 4.523 ± 0.077
0.422PheCys: 0.422 ± 0.02
2.713PheAsp: 2.713 ± 0.058
2.111PheGlu: 2.111 ± 0.051
1.644PhePhe: 1.644 ± 0.054
3.509PheGly: 3.509 ± 0.065
0.819PheHis: 0.819 ± 0.028
1.913PheIle: 1.913 ± 0.046
1.492PheLys: 1.492 ± 0.045
3.295PheLeu: 3.295 ± 0.074
0.835PheMet: 0.835 ± 0.034
1.405PheAsn: 1.405 ± 0.041
1.67PhePro: 1.67 ± 0.046
1.218PheGln: 1.218 ± 0.038
2.094PheArg: 2.094 ± 0.052
2.728PheSer: 2.728 ± 0.054
2.169PheThr: 2.169 ± 0.076
3.074PheVal: 3.074 ± 0.056
0.492PheTrp: 0.492 ± 0.026
1.041PheTyr: 1.041 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
7.899GlyAla: 7.899 ± 0.12
0.831GlyCys: 0.831 ± 0.033
3.904GlyAsp: 3.904 ± 0.075
4.239GlyGlu: 4.239 ± 0.07
3.388GlyPhe: 3.388 ± 0.058
6.419GlyGly: 6.419 ± 0.127
1.573GlyHis: 1.573 ± 0.045
4.398GlyIle: 4.398 ± 0.085
4.036GlyLys: 4.036 ± 0.069
7.208GlyLeu: 7.208 ± 0.103
2.21GlyMet: 2.21 ± 0.057
2.622GlyAsn: 2.622 ± 0.087
2.31GlyPro: 2.31 ± 0.049
2.669GlyGln: 2.669 ± 0.059
4.526GlyArg: 4.526 ± 0.093
4.266GlySer: 4.266 ± 0.082
4.515GlyThr: 4.515 ± 0.138
6.258GlyVal: 6.258 ± 0.084
1.189GlyTrp: 1.189 ± 0.039
2.263GlyTyr: 2.263 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
2.351HisAla: 2.351 ± 0.055
0.227HisCys: 0.227 ± 0.02
1.108HisAsp: 1.108 ± 0.043
1.072HisGlu: 1.072 ± 0.035
0.899HisPhe: 0.899 ± 0.032
1.728HisGly: 1.728 ± 0.051
0.62HisHis: 0.62 ± 0.026
1.146HisIle: 1.146 ± 0.036
0.723HisLys: 0.723 ± 0.033
2.077HisLeu: 2.077 ± 0.054
0.47HisMet: 0.47 ± 0.024
0.613HisAsn: 0.613 ± 0.027
1.266HisPro: 1.266 ± 0.044
0.801HisGln: 0.801 ± 0.035
1.353HisArg: 1.353 ± 0.036
1.018HisSer: 1.018 ± 0.031
1.098HisThr: 1.098 ± 0.039
1.44HisVal: 1.44 ± 0.043
0.307HisTrp: 0.307 ± 0.02
0.647HisTyr: 0.647 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
7.096IleAla: 7.096 ± 0.107
0.572IleCys: 0.572 ± 0.027
3.758IleAsp: 3.758 ± 0.073
3.871IleGlu: 3.871 ± 0.076
1.825IlePhe: 1.825 ± 0.052
4.819IleGly: 4.819 ± 0.086
1.059IleHis: 1.059 ± 0.036
2.52IleIle: 2.52 ± 0.054
2.332IleLys: 2.332 ± 0.054
4.167IleLeu: 4.167 ± 0.073
1.037IleMet: 1.037 ± 0.038
1.939IleAsn: 1.939 ± 0.047
2.524IlePro: 2.524 ± 0.057
1.634IleGln: 1.634 ± 0.043
3.224IleArg: 3.224 ± 0.061
3.414IleSer: 3.414 ± 0.069
3.305IleThr: 3.305 ± 0.077
4.212IleVal: 4.212 ± 0.078
0.553IleTrp: 0.553 ± 0.026
1.211IleTyr: 1.211 ± 0.038
0.0IleXaa: 0.0 ± 0.0
Lys
4.458LysAla: 4.458 ± 0.071
0.306LysCys: 0.306 ± 0.022
2.15LysAsp: 2.15 ± 0.059
2.106LysGlu: 2.106 ± 0.057
1.629LysPhe: 1.629 ± 0.055
2.72LysGly: 2.72 ± 0.06
1.081LysHis: 1.081 ± 0.035
2.467LysIle: 2.467 ± 0.056
2.389LysLys: 2.389 ± 0.076
4.705LysLeu: 4.705 ± 0.073
1.413LysMet: 1.413 ± 0.052
1.606LysAsn: 1.606 ± 0.046
2.629LysPro: 2.629 ± 0.058
1.8LysGln: 1.8 ± 0.054
3.158LysArg: 3.158 ± 0.066
2.851LysSer: 2.851 ± 0.068
2.666LysThr: 2.666 ± 0.055
3.157LysVal: 3.157 ± 0.071
0.551LysTrp: 0.551 ± 0.029
1.018LysTyr: 1.018 ± 0.04
0.0LysXaa: 0.0 ± 0.0
Leu
12.133LeuAla: 12.133 ± 0.157
0.871LeuCys: 0.871 ± 0.031
5.562LeuAsp: 5.562 ± 0.094
4.67LeuGlu: 4.67 ± 0.087
3.56LeuPhe: 3.56 ± 0.071
7.019LeuGly: 7.019 ± 0.096
1.998LeuHis: 1.998 ± 0.058
5.009LeuIle: 5.009 ± 0.091
4.545LeuLys: 4.545 ± 0.074
9.321LeuLeu: 9.321 ± 0.148
2.403LeuMet: 2.403 ± 0.056
3.299LeuAsn: 3.299 ± 0.071
5.191LeuPro: 5.191 ± 0.079
3.149LeuGln: 3.149 ± 0.08
6.003LeuArg: 6.003 ± 0.084
6.405LeuSer: 6.405 ± 0.082
5.856LeuThr: 5.856 ± 0.117
7.188LeuVal: 7.188 ± 0.103
1.003LeuTrp: 1.003 ± 0.036
2.137LeuTyr: 2.137 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
2.648MetAla: 2.648 ± 0.064
0.184MetCys: 0.184 ± 0.015
1.096MetAsp: 1.096 ± 0.035
1.167MetGlu: 1.167 ± 0.034
0.844MetPhe: 0.844 ± 0.036
1.861MetGly: 1.861 ± 0.055
0.563MetHis: 0.563 ± 0.025
1.267MetIle: 1.267 ± 0.042
1.382MetLys: 1.382 ± 0.044
2.72MetLeu: 2.72 ± 0.069
0.699MetMet: 0.699 ± 0.029
1.018MetAsn: 1.018 ± 0.035
1.481MetPro: 1.481 ± 0.04
1.077MetGln: 1.077 ± 0.031
1.753MetArg: 1.753 ± 0.046
1.781MetSer: 1.781 ± 0.047
1.693MetThr: 1.693 ± 0.046
1.877MetVal: 1.877 ± 0.049
0.248MetTrp: 0.248 ± 0.016
0.455MetTyr: 0.455 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
3.998AsnAla: 3.998 ± 0.082
0.359AsnCys: 0.359 ± 0.031
1.706AsnAsp: 1.706 ± 0.054
1.665AsnGlu: 1.665 ± 0.045
1.308AsnPhe: 1.308 ± 0.04
2.685AsnGly: 2.685 ± 0.076
0.667AsnHis: 0.667 ± 0.026
1.934AsnIle: 1.934 ± 0.048
1.295AsnLys: 1.295 ± 0.048
3.241AsnLeu: 3.241 ± 0.061
0.746AsnMet: 0.746 ± 0.03
1.17AsnAsn: 1.17 ± 0.043
2.178AsnPro: 2.178 ± 0.064
1.16AsnGln: 1.16 ± 0.04
2.075AsnArg: 2.075 ± 0.052
1.687AsnSer: 1.687 ± 0.054
1.996AsnThr: 1.996 ± 0.071
2.614AsnVal: 2.614 ± 0.051
0.486AsnTrp: 0.486 ± 0.024
0.824AsnTyr: 0.824 ± 0.036
0.0AsnXaa: 0.0 ± 0.0
Pro
5.196ProAla: 5.196 ± 0.095
0.328ProCys: 0.328 ± 0.018
2.634ProAsp: 2.634 ± 0.054
2.611ProGlu: 2.611 ± 0.058
1.932ProPhe: 1.932 ± 0.046
3.324ProGly: 3.324 ± 0.071
1.054ProHis: 1.054 ± 0.04
2.666ProIle: 2.666 ± 0.051
2.145ProLys: 2.145 ± 0.052
4.435ProLeu: 4.435 ± 0.077
1.277ProMet: 1.277 ± 0.04
1.908ProAsn: 1.908 ± 0.046
2.26ProPro: 2.26 ± 0.064
1.747ProGln: 1.747 ± 0.045
2.401ProArg: 2.401 ± 0.06
3.079ProSer: 3.079 ± 0.073
3.115ProThr: 3.115 ± 0.076
3.591ProVal: 3.591 ± 0.071
0.615ProTrp: 0.615 ± 0.029
1.257ProTyr: 1.257 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
3.943GlnAla: 3.943 ± 0.077
0.266GlnCys: 0.266 ± 0.017
1.523GlnAsp: 1.523 ± 0.041
1.441GlnGlu: 1.441 ± 0.045
1.42GlnPhe: 1.42 ± 0.043
2.256GlnGly: 2.256 ± 0.051
0.835GlnHis: 0.835 ± 0.033
2.116GlnIle: 2.116 ± 0.06
1.462GlnLys: 1.462 ± 0.042
3.783GlnLeu: 3.783 ± 0.073
1.041GlnMet: 1.041 ± 0.038
1.09GlnAsn: 1.09 ± 0.04
1.73GlnPro: 1.73 ± 0.044
1.583GlnGln: 1.583 ± 0.049
2.56GlnArg: 2.56 ± 0.063
2.318GlnSer: 2.318 ± 0.049
2.125GlnThr: 2.125 ± 0.057
2.635GlnVal: 2.635 ± 0.053
0.476GlnTrp: 0.476 ± 0.027
0.951GlnTyr: 0.951 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
6.497ArgAla: 6.497 ± 0.114
0.519ArgCys: 0.519 ± 0.028
3.598ArgAsp: 3.598 ± 0.073
3.894ArgGlu: 3.894 ± 0.072
2.722ArgPhe: 2.722 ± 0.065
4.295ArgGly: 4.295 ± 0.07
1.377ArgHis: 1.377 ± 0.041
3.605ArgIle: 3.605 ± 0.074
2.36ArgLys: 2.36 ± 0.053
6.41ArgLeu: 6.41 ± 0.096
1.758ArgMet: 1.758 ± 0.047
1.875ArgAsn: 1.875 ± 0.049
2.634ArgPro: 2.634 ± 0.059
2.376ArgGln: 2.376 ± 0.057
4.036ArgArg: 4.036 ± 0.081
3.043ArgSer: 3.043 ± 0.064
2.982ArgThr: 2.982 ± 0.052
4.621ArgVal: 4.621 ± 0.077
0.854ArgTrp: 0.854 ± 0.03
1.892ArgTyr: 1.892 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
6.809SerAla: 6.809 ± 0.117
0.566SerCys: 0.566 ± 0.033
2.86SerAsp: 2.86 ± 0.062
2.745SerGlu: 2.745 ± 0.064
2.321SerPhe: 2.321 ± 0.051
5.459SerGly: 5.459 ± 0.096
1.248SerHis: 1.248 ± 0.043
3.209SerIle: 3.209 ± 0.067
2.337SerLys: 2.337 ± 0.052
5.828SerLeu: 5.828 ± 0.079
1.49SerMet: 1.49 ± 0.046
2.028SerAsn: 2.028 ± 0.059
2.948SerPro: 2.948 ± 0.066
2.106SerGln: 2.106 ± 0.055
3.614SerArg: 3.614 ± 0.067
3.436SerSer: 3.436 ± 0.075
3.766SerThr: 3.766 ± 0.104
4.466SerVal: 4.466 ± 0.08
0.74SerTrp: 0.74 ± 0.031
1.402SerTyr: 1.402 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
6.227ThrAla: 6.227 ± 0.135
0.56ThrCys: 0.56 ± 0.044
2.803ThrAsp: 2.803 ± 0.065
2.586ThrGlu: 2.586 ± 0.057
2.306ThrPhe: 2.306 ± 0.059
4.763ThrGly: 4.763 ± 0.134
1.303ThrHis: 1.303 ± 0.039
3.358ThrIle: 3.358 ± 0.092
2.471ThrLys: 2.471 ± 0.057
6.31ThrLeu: 6.31 ± 0.1
1.28ThrMet: 1.28 ± 0.041
2.17ThrAsn: 2.17 ± 0.087
3.691ThrPro: 3.691 ± 0.078
1.973ThrGln: 1.973 ± 0.053
3.236ThrArg: 3.236 ± 0.069
3.545ThrSer: 3.545 ± 0.097
3.812ThrThr: 3.812 ± 0.159
4.476ThrVal: 4.476 ± 0.146
0.701ThrTrp: 0.701 ± 0.031
1.483ThrTyr: 1.483 ± 0.062
0.0ThrXaa: 0.0 ± 0.0
Val
9.027ValAla: 9.027 ± 0.111
0.786ValCys: 0.786 ± 0.035
4.051ValAsp: 4.051 ± 0.079
4.24ValGlu: 4.24 ± 0.069
2.905ValPhe: 2.905 ± 0.067
5.911ValGly: 5.911 ± 0.099
1.349ValHis: 1.349 ± 0.046
4.184ValIle: 4.184 ± 0.068
3.202ValLys: 3.202 ± 0.073
7.273ValLeu: 7.273 ± 0.11
2.069ValMet: 2.069 ± 0.054
2.527ValAsn: 2.527 ± 0.061
3.417ValPro: 3.417 ± 0.055
2.174ValGln: 2.174 ± 0.056
4.253ValArg: 4.253 ± 0.077
4.874ValSer: 4.874 ± 0.089
4.677ValThr: 4.677 ± 0.133
6.306ValVal: 6.306 ± 0.091
0.969ValTrp: 0.969 ± 0.036
1.667ValTyr: 1.667 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.992TrpAla: 0.992 ± 0.032
0.152TrpCys: 0.152 ± 0.015
0.636TrpAsp: 0.636 ± 0.025
0.564TrpGlu: 0.564 ± 0.025
0.53TrpPhe: 0.53 ± 0.026
0.867TrpGly: 0.867 ± 0.035
0.311TrpHis: 0.311 ± 0.017
0.668TrpIle: 0.668 ± 0.026
0.552TrpLys: 0.552 ± 0.023
1.711TrpLeu: 1.711 ± 0.05
0.403TrpMet: 0.403 ± 0.022
0.408TrpAsn: 0.408 ± 0.02
0.506TrpPro: 0.506 ± 0.027
0.701TrpGln: 0.701 ± 0.027
1.12TrpArg: 1.12 ± 0.038
0.758TrpSer: 0.758 ± 0.033
0.611TrpThr: 0.611 ± 0.031
0.957TrpVal: 0.957 ± 0.032
0.261TrpTrp: 0.261 ± 0.021
0.324TrpTyr: 0.324 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.771TyrAla: 2.771 ± 0.063
0.288TyrCys: 0.288 ± 0.02
1.354TyrAsp: 1.354 ± 0.04
1.203TyrGlu: 1.203 ± 0.035
1.144TyrPhe: 1.144 ± 0.035
1.962TyrGly: 1.962 ± 0.049
0.492TyrHis: 0.492 ± 0.026
1.071TyrIle: 1.071 ± 0.036
0.947TyrLys: 0.947 ± 0.04
2.534TyrLeu: 2.534 ± 0.061
0.437TyrMet: 0.437 ± 0.026
0.792TyrAsn: 0.792 ± 0.031
1.188TyrPro: 1.188 ± 0.041
1.019TyrGln: 1.019 ± 0.036
1.785TyrArg: 1.785 ± 0.046
1.403TyrSer: 1.403 ± 0.04
1.449TyrThr: 1.449 ± 0.05
1.838TyrVal: 1.838 ± 0.051
0.395TyrTrp: 0.395 ± 0.023
0.671TyrTyr: 0.671 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2745 proteins (861845 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski