Hydrogenimonas thermophila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Hydrogenimonaceae; Hydrogenimonas

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2512 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5QGM7|A0A1I5QGM7_9PROT Uncharacterized protein OS=Hydrogenimonas thermophila OX=223786 GN=SAMN05216234_1206 PE=4 SV=1
MM1 pKa = 7.87VFEE4 pKa = 4.78SVIKK8 pKa = 10.53AKK10 pKa = 10.29PLGGWYY16 pKa = 9.41IEE18 pKa = 4.32LTNTDD23 pKa = 2.76TGMSVVCDD31 pKa = 4.23SIDD34 pKa = 3.26EE35 pKa = 4.11YY36 pKa = 10.67MEE38 pKa = 4.93KK39 pKa = 9.52IEE41 pKa = 5.33EE42 pKa = 4.05MGAPYY47 pKa = 10.53GPDD50 pKa = 3.08VQVNWSQGEE59 pKa = 4.31GVIPAYY65 pKa = 9.97INEE68 pKa = 4.14IRR70 pKa = 11.84GLMQKK75 pKa = 9.24YY76 pKa = 8.63QEE78 pKa = 4.15EE79 pKa = 4.53AGLSEE84 pKa = 4.35GG85 pKa = 4.44

Molecular weight: 9.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I5P170|A0A1I5P170_9PROT Dynamin family protein OS=Hydrogenimonas thermophila OX=223786 GN=SAMN05216234_11314 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.34RR14 pKa = 11.84THH16 pKa = 6.22GFRR19 pKa = 11.84TRR21 pKa = 11.84MKK23 pKa = 9.0TKK25 pKa = 10.28NGRR28 pKa = 11.84KK29 pKa = 9.12IINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.62GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2512

0

2512

754384

25

1787

300.3

34.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.474 ± 0.057

0.909 ± 0.017

5.749 ± 0.04

7.533 ± 0.058

4.867 ± 0.039

5.803 ± 0.056

1.81 ± 0.022

8.872 ± 0.051

8.932 ± 0.059

9.609 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.022

5.091 ± 0.053

3.296 ± 0.028

2.833 ± 0.027

3.871 ± 0.038

6.157 ± 0.038

4.759 ± 0.039

6.144 ± 0.042

0.85 ± 0.017

3.941 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski