Paspalum striate mosaic virus
Average proteome isoelectric point is 7.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1CSA7|G1CSA7_9GEMI Replication-associated protein OS=Paspalum striate mosaic virus OX=1072672 PE=3 SV=1
MM1 pKa = 7.51 SSLVSEE7 pKa = 4.65 TSNSEE12 pKa = 3.66 VGSQMEE18 pKa = 4.29 SPGRR22 pKa = 11.84 GGQSIDD28 pKa = 4.11 APSSSCFKK36 pKa = 10.61 VRR38 pKa = 11.84 ARR40 pKa = 11.84 NLFLTYY46 pKa = 10.4 SKK48 pKa = 11.26 CNLTAVFLLEE58 pKa = 4.56 YY59 pKa = 10.31 ISSLLKK65 pKa = 10.36 KK66 pKa = 10.11 YY67 pKa = 10.27 CPTYY71 pKa = 10.32 IYY73 pKa = 10.34 VAQEE77 pKa = 3.55 AHH79 pKa = 7.0 KK80 pKa = 10.84 DD81 pKa = 3.84 GSHH84 pKa = 6.72 HH85 pKa = 6.01 LHH87 pKa = 7.26 CIIQCSKK94 pKa = 10.6 YY95 pKa = 10.89 VRR97 pKa = 11.84 TTSAKK102 pKa = 10.01 FFDD105 pKa = 3.42 IKK107 pKa = 10.64 EE108 pKa = 4.03 FHH110 pKa = 7.32 PNVQNPRR117 pKa = 11.84 MPKK120 pKa = 9.95 KK121 pKa = 10.49 ALSYY125 pKa = 10.07 CKK127 pKa = 10.06 KK128 pKa = 10.52 SPISEE133 pKa = 4.09 AEE135 pKa = 3.75 YY136 pKa = 10.93 GVFQEE141 pKa = 4.71 IKK143 pKa = 10.24 RR144 pKa = 11.84 PRR146 pKa = 11.84 KK147 pKa = 9.52 KK148 pKa = 10.52 KK149 pKa = 10.51 ADD151 pKa = 3.62 APSTKK156 pKa = 9.4 DD157 pKa = 2.93 AKK159 pKa = 9.68 MAEE162 pKa = 4.41 IIKK165 pKa = 10.69 SSTNKK170 pKa = 9.84 EE171 pKa = 4.34 DD172 pKa = 3.65 YY173 pKa = 11.07 LSMVRR178 pKa = 11.84 KK179 pKa = 10.02 SFPFDD184 pKa = 2.63 WATRR188 pKa = 11.84 LQQFQFSAEE197 pKa = 4.17 SLFPSTPPPYY207 pKa = 10.16 VDD209 pKa = 3.41 PFGMPSQDD217 pKa = 2.97 THH219 pKa = 6.58 PVIGAWLRR227 pKa = 11.84 DD228 pKa = 3.46 EE229 pKa = 5.67 LYY231 pKa = 10.07 TVSPYY236 pKa = 10.84 AYY238 pKa = 9.12 SIHH241 pKa = 6.61 NGVSEE246 pKa = 4.27 EE247 pKa = 3.99 QAKK250 pKa = 10.77 LDD252 pKa = 3.97 LQWMSDD258 pKa = 3.34 LSRR261 pKa = 11.84 TEE263 pKa = 3.67 VLLNEE268 pKa = 5.02 DD269 pKa = 3.51 VPSTYY274 pKa = 10.35 VVQQEE279 pKa = 4.0 QEE281 pKa = 3.9 RR282 pKa = 11.84 LLGQEE287 pKa = 3.83 VSEE290 pKa = 4.98 VITTGNIPWTSSTT303 pKa = 3.81
Molecular weight: 34.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.44
IPC2_protein 6.504
IPC_protein 6.649
Toseland 6.664
ProMoST 7.0
Dawson 7.146
Bjellqvist 7.044
Wikipedia 7.117
Rodwell 7.146
Grimsley 6.751
Solomon 7.19
Lehninger 7.19
Nozaki 7.366
DTASelect 7.424
Thurlkill 7.468
EMBOSS 7.497
Sillero 7.556
Patrickios 4.075
IPC_peptide 7.19
IPC2_peptide 7.0
IPC2.peptide.svr19 6.952
Protein with the highest isoelectric point:
>tr|G1CSA6|G1CSA6_9GEMI Replication-associated protein OS=Paspalum striate mosaic virus OX=1072672 PE=3 SV=1
MM1 pKa = 7.39 AASSKK6 pKa = 10.15 RR7 pKa = 11.84 KK8 pKa = 9.68 RR9 pKa = 11.84 GSTSATKK16 pKa = 9.95 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 9.37 KK20 pKa = 10.11 PRR22 pKa = 11.84 YY23 pKa = 7.2 TKK25 pKa = 8.14 WTSARR30 pKa = 11.84 SSNRR34 pKa = 11.84 DD35 pKa = 3.08 ALQVQTFTWGEE46 pKa = 3.91 DD47 pKa = 3.25 QAFNAGGRR55 pKa = 11.84 AILLTAFTRR64 pKa = 11.84 GSAEE68 pKa = 3.99 NQRR71 pKa = 11.84 KK72 pKa = 7.88 SQEE75 pKa = 4.03 TITYY79 pKa = 9.06 KK80 pKa = 10.68 VSINLGVSASATVLKK95 pKa = 9.77 YY96 pKa = 10.38 CCKK99 pKa = 10.26 SQPLCWLVYY108 pKa = 10.14 DD109 pKa = 4.87 KK110 pKa = 11.28 SPTGITDD117 pKa = 3.98 LTPSDD122 pKa = 3.74 IFDD125 pKa = 3.86 VPSGLQNWPSTWKK138 pKa = 9.85 VKK140 pKa = 10.64 RR141 pKa = 11.84 EE142 pKa = 3.66 ASHH145 pKa = 6.57 RR146 pKa = 11.84 FVVKK150 pKa = 10.34 RR151 pKa = 11.84 RR152 pKa = 11.84 WPFTLSCNGSTFQKK166 pKa = 10.53 DD167 pKa = 3.78 YY168 pKa = 11.5 TNLPVCNTDD177 pKa = 2.96 NLVSVTRR184 pKa = 11.84 FAKK187 pKa = 10.25 GLGVRR192 pKa = 11.84 TEE194 pKa = 4.04 WKK196 pKa = 8.74 DD197 pKa = 3.15 TTTADD202 pKa = 3.56 ASDD205 pKa = 3.4 IKK207 pKa = 11.11 GGALYY212 pKa = 10.66 LVVAPANGLVFTARR226 pKa = 11.84 GVIKK230 pKa = 10.61 VYY232 pKa = 10.27 FKK234 pKa = 11.22 SVGNQQ239 pKa = 2.8
Molecular weight: 26.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.215
IPC2_protein 9.472
IPC_protein 9.516
Toseland 10.335
ProMoST 9.911
Dawson 10.467
Bjellqvist 10.101
Wikipedia 10.599
Rodwell 10.994
Grimsley 10.511
Solomon 10.496
Lehninger 10.482
Nozaki 10.335
DTASelect 10.087
Thurlkill 10.35
EMBOSS 10.716
Sillero 10.379
Patrickios 10.687
IPC_peptide 10.511
IPC2_peptide 8.858
IPC2.peptide.svr19 8.436
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1017
111
364
254.3
28.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.276 ± 1.421
2.065 ± 0.249
4.326 ± 0.501
4.818 ± 1.014
4.818 ± 0.35
4.72 ± 0.68
1.77 ± 0.607
4.621 ± 0.604
7.08 ± 1.028
6.883 ± 0.166
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.672 ± 0.462
3.343 ± 0.744
5.998 ± 0.747
4.425 ± 0.421
5.211 ± 0.677
11.504 ± 0.864
6.785 ± 1.311
6.981 ± 1.009
1.77 ± 0.5
3.933 ± 0.556
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here