Mesoplasma lactucae ATCC 49193

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Entomoplasmataceae; Mesoplasma; Mesoplasma lactucae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 689 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291IRH1|A0A291IRH1_9MOLU Uncharacterized protein OS=Mesoplasma lactucae ATCC 49193 OX=81460 GN=CP520_00745 PE=4 SV=1
MM1 pKa = 8.5SEE3 pKa = 3.84MFFYY7 pKa = 11.08NHH9 pKa = 6.09TKK11 pKa = 9.81MDD13 pKa = 3.52MTDD16 pKa = 3.57WEE18 pKa = 4.41DD19 pKa = 3.54LYY21 pKa = 12.04NNLFITAKK29 pKa = 10.44EE30 pKa = 3.84ILNLEE35 pKa = 4.21NNNLSLAITWVDD47 pKa = 3.07KK48 pKa = 11.41PEE50 pKa = 3.93ALKK53 pKa = 11.02LNQEE57 pKa = 4.24YY58 pKa = 9.89RR59 pKa = 11.84HH60 pKa = 5.5QEE62 pKa = 3.86YY63 pKa = 10.44VPDD66 pKa = 4.26VISFPTEE73 pKa = 3.43MDD75 pKa = 3.37EE76 pKa = 4.74EE77 pKa = 4.57LVKK80 pKa = 11.01EE81 pKa = 4.18MGEE84 pKa = 4.23HH85 pKa = 6.05EE86 pKa = 4.87LGDD89 pKa = 4.38LFICYY94 pKa = 9.92DD95 pKa = 3.63VALEE99 pKa = 4.18KK100 pKa = 10.54ADD102 pKa = 3.99KK103 pKa = 9.22YY104 pKa = 10.34HH105 pKa = 6.56HH106 pKa = 6.61NIYY109 pKa = 10.99EE110 pKa = 3.95EE111 pKa = 4.0MAFLFVHH118 pKa = 6.73GFLHH122 pKa = 6.98LLGYY126 pKa = 10.66DD127 pKa = 3.44HH128 pKa = 7.69DD129 pKa = 5.63LSLEE133 pKa = 4.13DD134 pKa = 3.84EE135 pKa = 4.66KK136 pKa = 11.66EE137 pKa = 3.92MFTIQDD143 pKa = 4.51KK144 pKa = 10.69ILKK147 pKa = 9.49AYY149 pKa = 9.48NINYY153 pKa = 8.76EE154 pKa = 4.17VIYY157 pKa = 10.74DD158 pKa = 3.99DD159 pKa = 4.91EE160 pKa = 7.25DD161 pKa = 3.92YY162 pKa = 11.61LL163 pKa = 5.43

Molecular weight: 19.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291ISL1|A0A291ISL1_9MOLU 30S ribosomal protein S18 OS=Mesoplasma lactucae ATCC 49193 OX=81460 GN=rpsR PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.46IKK11 pKa = 9.25TAHH14 pKa = 4.44THH16 pKa = 4.99GFRR19 pKa = 11.84ARR21 pKa = 11.84MASKK25 pKa = 10.57NGRR28 pKa = 11.84KK29 pKa = 9.28VIKK32 pKa = 10.14ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.34GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

689

0

689

246924

37

2475

358.4

40.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.1 ± 0.097

0.517 ± 0.021

6.477 ± 0.071

6.544 ± 0.108

4.709 ± 0.094

5.65 ± 0.081

1.362 ± 0.038

8.344 ± 0.079

9.317 ± 0.089

8.894 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.451 ± 0.05

6.982 ± 0.1

3.062 ± 0.04

3.486 ± 0.055

3.121 ± 0.069

5.76 ± 0.082

5.757 ± 0.082

6.348 ± 0.075

1.106 ± 0.04

4.012 ± 0.074
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski