East African cassava mosaic virus-KE2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; East African cassava mosaic virus

Average proteome isoelectric point is 7.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2A8X4|Q2A8X4_9GEMI Protein V2 OS=East African cassava mosaic virus-KE2 OX=374778 GN=AV2 PE=3 SV=1
MM1 pKa = 8.09WDD3 pKa = 3.51PLLNDD8 pKa = 3.72FPEE11 pKa = 4.7TVHH14 pKa = 6.61GFRR17 pKa = 11.84SMLAVKK23 pKa = 10.21YY24 pKa = 10.06LLHH27 pKa = 7.24LEE29 pKa = 4.05QEE31 pKa = 4.43YY32 pKa = 11.26DD33 pKa = 3.21RR34 pKa = 11.84GTVGAEE40 pKa = 4.07YY41 pKa = 10.14IRR43 pKa = 11.84DD44 pKa = 3.91LIGVLRR50 pKa = 11.84CKK52 pKa = 10.54SYY54 pKa = 11.16VEE56 pKa = 3.55ATRR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 9.31NNLNTRR67 pKa = 11.84IQGAEE72 pKa = 3.96EE73 pKa = 4.16AEE75 pKa = 4.18LRR77 pKa = 11.84QPIHH81 pKa = 6.58EE82 pKa = 4.56PCCCPHH88 pKa = 6.94CPRR91 pKa = 11.84HH92 pKa = 5.47QKK94 pKa = 10.61QNMGQQAHH102 pKa = 5.32VSEE105 pKa = 4.72AQDD108 pKa = 3.39VQNVSKK114 pKa = 10.21PRR116 pKa = 11.84CSS118 pKa = 3.17

Molecular weight: 13.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2A8X4|Q2A8X4_9GEMI Protein V2 OS=East African cassava mosaic virus-KE2 OX=374778 GN=AV2 PE=3 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.44RR4 pKa = 11.84PGDD7 pKa = 3.95IIISTPVSKK16 pKa = 10.56VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.03SPYY27 pKa = 8.09TNRR30 pKa = 11.84VVAPTVRR37 pKa = 11.84VTRR40 pKa = 11.84SKK42 pKa = 10.08IWANRR47 pKa = 11.84PMYY50 pKa = 9.87RR51 pKa = 11.84KK52 pKa = 8.82PKK54 pKa = 7.7MYY56 pKa = 10.61RR57 pKa = 11.84MYY59 pKa = 10.67RR60 pKa = 11.84SPDD63 pKa = 3.29VPKK66 pKa = 10.81GCEE69 pKa = 4.39GPCKK73 pKa = 9.37VQSYY77 pKa = 8.49EE78 pKa = 3.72QGDD81 pKa = 4.21DD82 pKa = 3.53VKK84 pKa = 10.01HH85 pKa = 5.79TGMVRR90 pKa = 11.84CVSDD94 pKa = 3.26VTRR97 pKa = 11.84GPGITHH103 pKa = 6.76RR104 pKa = 11.84VGKK107 pKa = 9.5RR108 pKa = 11.84FCVKK112 pKa = 10.3SIYY115 pKa = 10.1ILGKK119 pKa = 9.09IWMDD123 pKa = 3.64EE124 pKa = 4.09NIKK127 pKa = 10.06KK128 pKa = 9.97QNHH131 pKa = 5.06TNHH134 pKa = 5.22VMFFLVRR141 pKa = 11.84DD142 pKa = 3.77RR143 pKa = 11.84RR144 pKa = 11.84PYY146 pKa = 10.68GPSPQDD152 pKa = 3.16FGQVFNMFDD161 pKa = 3.83NEE163 pKa = 4.14PTTATVKK170 pKa = 10.66NDD172 pKa = 2.87LRR174 pKa = 11.84DD175 pKa = 3.47RR176 pKa = 11.84YY177 pKa = 9.53QVLRR181 pKa = 11.84KK182 pKa = 9.66FYY184 pKa = 8.73ATVVGGPSGMKK195 pKa = 9.47EE196 pKa = 3.54QALVKK201 pKa = 10.41RR202 pKa = 11.84FFRR205 pKa = 11.84INNHH209 pKa = 4.14VVYY212 pKa = 10.6NHH214 pKa = 5.79QEE216 pKa = 3.59QAKK219 pKa = 9.66YY220 pKa = 10.54EE221 pKa = 4.12NHH223 pKa = 6.33TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.86ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.65FYY252 pKa = 11.26DD253 pKa = 3.34AVTNN257 pKa = 4.16

Molecular weight: 29.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1702

77

359

212.8

24.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.818 ± 0.624

2.056 ± 0.342

5.053 ± 0.292

4.289 ± 0.521

4.759 ± 0.52

5.288 ± 0.827

3.937 ± 0.68

5.875 ± 0.725

5.523 ± 0.521

7.462 ± 0.669

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.35 ± 0.415

5.229 ± 0.391

6.228 ± 0.373

4.289 ± 0.657

7.051 ± 0.743

8.519 ± 1.023

5.875 ± 0.489

6.287 ± 0.858

1.058 ± 0.171

4.054 ± 0.676
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski