Streptococcus phage Javan266

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AXG9|A0A4D6AXG9_9CAUD Uncharacterized protein OS=Streptococcus phage Javan266 OX=2548082 GN=Javan266_0022 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 4.97GFFNYY7 pKa = 9.79DD8 pKa = 3.65RR9 pKa = 11.84DD10 pKa = 3.88MMDD13 pKa = 3.35PPEE16 pKa = 4.25EE17 pKa = 4.29RR18 pKa = 11.84EE19 pKa = 3.93EE20 pKa = 4.49LDD22 pKa = 3.38PEE24 pKa = 4.52LYY26 pKa = 10.7VYY28 pKa = 10.05IGCGQYY34 pKa = 10.39RR35 pKa = 11.84YY36 pKa = 10.72VGDD39 pKa = 4.28EE40 pKa = 3.68II41 pKa = 5.83

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AYF6|A0A4D6AYF6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan266 OX=2548082 GN=Javan266_0024 PE=4 SV=1
MM1 pKa = 8.42IEE3 pKa = 4.1EE4 pKa = 4.32LLAEE8 pKa = 4.61IGQWRR13 pKa = 11.84SDD15 pKa = 3.9YY16 pKa = 10.58IHH18 pKa = 7.1LGIEE22 pKa = 3.64LGKK25 pKa = 10.33IINEE29 pKa = 3.96QQDD32 pKa = 3.97IIVKK36 pKa = 9.26LQNEE40 pKa = 3.95NRR42 pKa = 11.84RR43 pKa = 11.84LKK45 pKa = 10.76RR46 pKa = 11.84EE47 pKa = 3.62NRR49 pKa = 11.84NLKK52 pKa = 7.89KK53 pKa = 9.47TKK55 pKa = 9.57GRR57 pKa = 11.84KK58 pKa = 8.57KK59 pKa = 10.52

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10357

39

1233

199.2

22.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.266 ± 0.471

0.531 ± 0.099

6.517 ± 0.267

8.159 ± 0.499

4.171 ± 0.258

6.797 ± 0.661

1.448 ± 0.173

6.701 ± 0.321

8.246 ± 0.456

8.226 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.404 ± 0.227

5.407 ± 0.195

2.887 ± 0.286

4.181 ± 0.203

4.606 ± 0.291

5.87 ± 0.24

5.697 ± 0.35

6.479 ± 0.281

1.313 ± 0.133

4.094 ± 0.351
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski