Microbacterium phage Celaena

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dismasvirus; unclassified Dismasvirus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DFN8|A0A514DFN8_9CAUD DNA primase/helicase OS=Microbacterium phage Celaena OX=2591214 GN=39 PE=4 SV=1
MM1 pKa = 7.47NNNDD5 pKa = 3.57TSRR8 pKa = 11.84LPHH11 pKa = 6.51EE12 pKa = 4.24VTLAIVQRR20 pKa = 11.84ADD22 pKa = 3.31QMVDD26 pKa = 3.47DD27 pKa = 5.11FLADD31 pKa = 3.43EE32 pKa = 5.2GEE34 pKa = 4.49TLSSAGILLSVIEE47 pKa = 4.43VVSDD51 pKa = 3.41NAGFEE56 pKa = 3.85IEE58 pKa = 5.53LRR60 pKa = 11.84ITKK63 pKa = 10.19CGYY66 pKa = 9.86QLAFPQSVTVDD77 pKa = 3.56DD78 pKa = 4.19VLDD81 pKa = 4.66NIDD84 pKa = 3.6RR85 pKa = 11.84GFEE88 pKa = 4.11VMHH91 pKa = 5.36EE92 pKa = 4.46TIYY95 pKa = 11.38NPIDD99 pKa = 3.14

Molecular weight: 11.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DFK9|A0A514DFK9_9CAUD Uncharacterized protein OS=Microbacterium phage Celaena OX=2591214 GN=23 PE=4 SV=1
MM1 pKa = 7.51AGAVKK6 pKa = 9.97KK7 pKa = 11.02VRR9 pKa = 11.84MNPAGVVALLKK20 pKa = 10.87SSGVQADD27 pKa = 3.89LSARR31 pKa = 11.84GQRR34 pKa = 11.84VQSALPTSDD43 pKa = 3.31GEE45 pKa = 4.2EE46 pKa = 3.89WAVDD50 pKa = 3.39SFLGYY55 pKa = 10.73DD56 pKa = 3.5RR57 pKa = 11.84AQTVVRR63 pKa = 11.84TGNAAARR70 pKa = 11.84RR71 pKa = 11.84TQAEE75 pKa = 4.2TNALLRR81 pKa = 11.84ALDD84 pKa = 3.57RR85 pKa = 11.84GRR87 pKa = 4.36

Molecular weight: 9.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13187

39

925

206.0

22.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.382 ± 0.389

0.402 ± 0.082

6.34 ± 0.286

6.302 ± 0.286

3.284 ± 0.179

8.615 ± 0.388

1.782 ± 0.154

4.231 ± 0.207

4.186 ± 0.223

8.44 ± 0.341

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.017 ± 0.128

3.056 ± 0.246

5.24 ± 0.198

3.875 ± 0.162

6.431 ± 0.354

5.437 ± 0.269

6.446 ± 0.366

7.818 ± 0.278

2.146 ± 0.164

2.571 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski