Bacteroides sp. CAG:144
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2103 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R5YAR8|R5YAR8_9BACE PadR domain-containing protein OS=Bacteroides sp. CAG:144 OX=1262736 GN=BN496_01736 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.39 KK3 pKa = 10.32 KK4 pKa = 10.65 LFLAMSLAMGLFVATSCSDD23 pKa = 3.56 DD24 pKa = 5.24 DD25 pKa = 4.55 NDD27 pKa = 4.95 GGNGGNPTINSNYY40 pKa = 9.85 DD41 pKa = 3.32 LAYY44 pKa = 10.2 SAEE47 pKa = 4.16 NATAWGNYY55 pKa = 4.86 MVRR58 pKa = 11.84 VAEE61 pKa = 4.55 LLRR64 pKa = 11.84 DD65 pKa = 3.94 DD66 pKa = 5.38 AATLYY71 pKa = 11.14 ADD73 pKa = 3.95 WNSSYY78 pKa = 11.61 NGGASYY84 pKa = 11.51 ASIFKK89 pKa = 10.21 SHH91 pKa = 6.69 NNDD94 pKa = 3.01 TYY96 pKa = 11.59 GSALSCIEE104 pKa = 4.55 EE105 pKa = 4.55 IIDD108 pKa = 3.9 GCVTIADD115 pKa = 3.71 EE116 pKa = 5.03 VGDD119 pKa = 4.07 NKK121 pKa = 10.99 LGDD124 pKa = 4.1 PYY126 pKa = 11.38 DD127 pKa = 5.28 LYY129 pKa = 10.91 MQGNTTEE136 pKa = 3.87 ALYY139 pKa = 10.53 AVEE142 pKa = 5.54 SWYY145 pKa = 10.67 SWHH148 pKa = 7.26 SRR150 pKa = 11.84 DD151 pKa = 5.65 DD152 pKa = 3.48 YY153 pKa = 11.73 TNNIWSIRR161 pKa = 11.84 NAYY164 pKa = 9.77 FGSLDD169 pKa = 3.41 STVNANSISALVASVNTSLDD189 pKa = 3.61 EE190 pKa = 4.09 QMKK193 pKa = 8.85 TAILNAAAAIQAIPQPLRR211 pKa = 11.84 NNINSNEE218 pKa = 4.04 TLEE221 pKa = 4.88 AMEE224 pKa = 4.68 ACADD228 pKa = 4.17 LSAALSTLKK237 pKa = 10.61 KK238 pKa = 10.47 YY239 pKa = 10.48 IQDD242 pKa = 3.81 NLSDD246 pKa = 3.63 NAALEE251 pKa = 4.84 GIIDD255 pKa = 3.99 TYY257 pKa = 11.15 VDD259 pKa = 4.39 DD260 pKa = 5.64 VILPTYY266 pKa = 10.51 QSLKK270 pKa = 10.2 EE271 pKa = 4.23 KK272 pKa = 11.06 NSDD275 pKa = 3.39 LYY277 pKa = 11.33 DD278 pKa = 3.5 AVVAFRR284 pKa = 11.84 SNPSNAAFEE293 pKa = 4.63 TACHH297 pKa = 5.43 AWIEE301 pKa = 4.3 ARR303 pKa = 11.84 EE304 pKa = 3.97 PWEE307 pKa = 3.89 KK308 pKa = 11.11 SEE310 pKa = 4.78 AFLFGPVDD318 pKa = 4.4 VEE320 pKa = 4.35 GLDD323 pKa = 3.95 PNMDD327 pKa = 3.55 SWPLDD332 pKa = 2.87 VDD334 pKa = 4.66 AIVQILTTGNFGALDD349 pKa = 3.59 WDD351 pKa = 4.41 DD352 pKa = 5.12 DD353 pKa = 4.57 SEE355 pKa = 6.09 AEE357 pKa = 3.93 AAQSVRR363 pKa = 11.84 GFHH366 pKa = 5.46 TLEE369 pKa = 3.97 FLLFQNGAPRR379 pKa = 11.84 TIEE382 pKa = 3.73
Molecular weight: 41.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.897
IPC_protein 3.923
Toseland 3.706
ProMoST 4.05
Dawson 3.897
Bjellqvist 4.075
Wikipedia 3.821
Rodwell 3.745
Grimsley 3.605
Solomon 3.897
Lehninger 3.859
Nozaki 4.012
DTASelect 4.253
Thurlkill 3.745
EMBOSS 3.834
Sillero 4.037
Patrickios 1.367
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.924
Protein with the highest isoelectric point:
>tr|R5Y9F7|R5Y9F7_9BACE SAF domain-containing protein OS=Bacteroides sp. CAG:144 OX=1262736 GN=BN496_01316 PE=3 SV=1
MM1 pKa = 6.22 MRR3 pKa = 11.84 AWARR7 pKa = 11.84 NVSSPSFRR15 pKa = 11.84 LMEE18 pKa = 3.85 LTIPFPCIHH27 pKa = 6.86 FSPARR32 pKa = 11.84 MTGHH36 pKa = 6.5 FEE38 pKa = 3.55 EE39 pKa = 5.49 SIIIGTRR46 pKa = 11.84 QISGSEE52 pKa = 3.55 AMRR55 pKa = 11.84 FRR57 pKa = 11.84 NVTISFCASSMASSILMSMICAPSSTCLRR86 pKa = 11.84 AIPRR90 pKa = 11.84 ASSYY94 pKa = 10.44 FFSEE98 pKa = 4.19 ISRR101 pKa = 11.84 KK102 pKa = 7.63 NLRR105 pKa = 11.84 EE106 pKa = 3.69 PATLHH111 pKa = 6.88 RR112 pKa = 11.84 SPTLIKK118 pKa = 10.26 LFSGMIFNSSKK129 pKa = 10.18 PDD131 pKa = 3.41 KK132 pKa = 10.75 QSTFSLICGRR142 pKa = 4.19
Molecular weight: 15.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.414
IPC_protein 10.131
Toseland 10.672
ProMoST 10.833
Dawson 10.745
Bjellqvist 10.482
Wikipedia 10.95
Rodwell 10.862
Grimsley 10.774
Solomon 10.906
Lehninger 10.877
Nozaki 10.716
DTASelect 10.452
Thurlkill 10.672
EMBOSS 11.082
Sillero 10.701
Patrickios 10.657
IPC_peptide 10.921
IPC2_peptide 9.97
IPC2.peptide.svr19 8.796
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2103
0
2103
691520
29
1880
328.8
37.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.257 ± 0.053
1.336 ± 0.021
5.65 ± 0.043
6.387 ± 0.047
4.669 ± 0.039
6.954 ± 0.048
1.945 ± 0.024
7.324 ± 0.052
5.738 ± 0.044
9.094 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.537 ± 0.025
4.587 ± 0.037
3.909 ± 0.025
3.168 ± 0.029
5.292 ± 0.04
6.316 ± 0.041
5.631 ± 0.047
6.621 ± 0.046
1.147 ± 0.02
4.434 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here