Bacteroides sp. CAG:144

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|R5YAR8|R5YAR8_9BACE PadR domain-containing protein OS=Bacteroides sp. CAG:144 OX=1262736 GN=BN496_01736 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.39KK3 pKa = 10.32KK4 pKa = 10.65LFLAMSLAMGLFVATSCSDD23 pKa = 3.56DD24 pKa = 5.24DD25 pKa = 4.55NDD27 pKa = 4.95GGNGGNPTINSNYY40 pKa = 9.85DD41 pKa = 3.32LAYY44 pKa = 10.2SAEE47 pKa = 4.16NATAWGNYY55 pKa = 4.86MVRR58 pKa = 11.84VAEE61 pKa = 4.55LLRR64 pKa = 11.84DD65 pKa = 3.94DD66 pKa = 5.38AATLYY71 pKa = 11.14ADD73 pKa = 3.95WNSSYY78 pKa = 11.61NGGASYY84 pKa = 11.51ASIFKK89 pKa = 10.21SHH91 pKa = 6.69NNDD94 pKa = 3.01TYY96 pKa = 11.59GSALSCIEE104 pKa = 4.55EE105 pKa = 4.55IIDD108 pKa = 3.9GCVTIADD115 pKa = 3.71EE116 pKa = 5.03VGDD119 pKa = 4.07NKK121 pKa = 10.99LGDD124 pKa = 4.1PYY126 pKa = 11.38DD127 pKa = 5.28LYY129 pKa = 10.91MQGNTTEE136 pKa = 3.87ALYY139 pKa = 10.53AVEE142 pKa = 5.54SWYY145 pKa = 10.67SWHH148 pKa = 7.26SRR150 pKa = 11.84DD151 pKa = 5.65DD152 pKa = 3.48YY153 pKa = 11.73TNNIWSIRR161 pKa = 11.84NAYY164 pKa = 9.77FGSLDD169 pKa = 3.41STVNANSISALVASVNTSLDD189 pKa = 3.61EE190 pKa = 4.09QMKK193 pKa = 8.85TAILNAAAAIQAIPQPLRR211 pKa = 11.84NNINSNEE218 pKa = 4.04TLEE221 pKa = 4.88AMEE224 pKa = 4.68ACADD228 pKa = 4.17LSAALSTLKK237 pKa = 10.61KK238 pKa = 10.47YY239 pKa = 10.48IQDD242 pKa = 3.81NLSDD246 pKa = 3.63NAALEE251 pKa = 4.84GIIDD255 pKa = 3.99TYY257 pKa = 11.15VDD259 pKa = 4.39DD260 pKa = 5.64VILPTYY266 pKa = 10.51QSLKK270 pKa = 10.2EE271 pKa = 4.23KK272 pKa = 11.06NSDD275 pKa = 3.39LYY277 pKa = 11.33DD278 pKa = 3.5AVVAFRR284 pKa = 11.84SNPSNAAFEE293 pKa = 4.63TACHH297 pKa = 5.43AWIEE301 pKa = 4.3ARR303 pKa = 11.84EE304 pKa = 3.97PWEE307 pKa = 3.89KK308 pKa = 11.11SEE310 pKa = 4.78AFLFGPVDD318 pKa = 4.4VEE320 pKa = 4.35GLDD323 pKa = 3.95PNMDD327 pKa = 3.55SWPLDD332 pKa = 2.87VDD334 pKa = 4.66AIVQILTTGNFGALDD349 pKa = 3.59WDD351 pKa = 4.41DD352 pKa = 5.12DD353 pKa = 4.57SEE355 pKa = 6.09AEE357 pKa = 3.93AAQSVRR363 pKa = 11.84GFHH366 pKa = 5.46TLEE369 pKa = 3.97FLLFQNGAPRR379 pKa = 11.84TIEE382 pKa = 3.73

Molecular weight:
41.74 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|R5Y9F7|R5Y9F7_9BACE SAF domain-containing protein OS=Bacteroides sp. CAG:144 OX=1262736 GN=BN496_01316 PE=3 SV=1
MM1 pKa = 6.22MRR3 pKa = 11.84AWARR7 pKa = 11.84NVSSPSFRR15 pKa = 11.84LMEE18 pKa = 3.85LTIPFPCIHH27 pKa = 6.86FSPARR32 pKa = 11.84MTGHH36 pKa = 6.5FEE38 pKa = 3.55EE39 pKa = 5.49SIIIGTRR46 pKa = 11.84QISGSEE52 pKa = 3.55AMRR55 pKa = 11.84FRR57 pKa = 11.84NVTISFCASSMASSILMSMICAPSSTCLRR86 pKa = 11.84AIPRR90 pKa = 11.84ASSYY94 pKa = 10.44FFSEE98 pKa = 4.19ISRR101 pKa = 11.84KK102 pKa = 7.63NLRR105 pKa = 11.84EE106 pKa = 3.69PATLHH111 pKa = 6.88RR112 pKa = 11.84SPTLIKK118 pKa = 10.26LFSGMIFNSSKK129 pKa = 10.18PDD131 pKa = 3.41KK132 pKa = 10.75QSTFSLICGRR142 pKa = 4.19

Molecular weight:
15.9 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2103

0

2103

691520

29

1880

328.8

37.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.257 ± 0.053

1.336 ± 0.021

5.65 ± 0.043

6.387 ± 0.047

4.669 ± 0.039

6.954 ± 0.048

1.945 ± 0.024

7.324 ± 0.052

5.738 ± 0.044

9.094 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.537 ± 0.025

4.587 ± 0.037

3.909 ± 0.025

3.168 ± 0.029

5.292 ± 0.04

6.316 ± 0.041

5.631 ± 0.047

6.621 ± 0.046

1.147 ± 0.02

4.434 ± 0.041

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski