Lacunisphaera limnophila

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Opitutae; Opitutales; Opitutaceae; Lacunisphaera

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3510 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8AZW3|A0A1D8AZW3_9BACT DNA polymerase IV OS=Lacunisphaera limnophila OX=1838286 GN=dinB_3 PE=3 SV=1
MM1 pKa = 7.92EE2 pKa = 5.58ILLILAAFVIFGAALWYY19 pKa = 10.04IPSPKK24 pKa = 9.84PRR26 pKa = 11.84DD27 pKa = 3.71DD28 pKa = 4.96RR29 pKa = 11.84GDD31 pKa = 3.11TSLPYY36 pKa = 10.1TGGDD40 pKa = 3.42TSSSTPVHH48 pKa = 6.95SDD50 pKa = 2.92PCSYY54 pKa = 11.25GHH56 pKa = 7.59DD57 pKa = 3.87FGSDD61 pKa = 2.91GGGDD65 pKa = 3.55CGGDD69 pKa = 3.31GGGGGSDD76 pKa = 3.13

Molecular weight: 7.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8AUR0|A0A1D8AUR0_9BACT Chromosome partition protein Smc OS=Lacunisphaera limnophila OX=1838286 GN=smc_2 PE=4 SV=1
MM1 pKa = 7.59GNLKK5 pKa = 10.05KK6 pKa = 10.26KK7 pKa = 10.28RR8 pKa = 11.84RR9 pKa = 11.84LKK11 pKa = 9.46MSKK14 pKa = 9.18HH15 pKa = 5.32KK16 pKa = 10.14RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.11RR20 pKa = 11.84LKK22 pKa = 10.0ANRR25 pKa = 11.84HH26 pKa = 5.49KK27 pKa = 10.88KK28 pKa = 8.96RR29 pKa = 11.84TWQKK33 pKa = 10.47

Molecular weight: 4.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3510

0

3510

1259279

29

8933

358.8

39.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.24 ± 0.059

0.861 ± 0.013

5.055 ± 0.047

5.332 ± 0.038

3.948 ± 0.031

8.357 ± 0.06

2.217 ± 0.023

4.439 ± 0.036

3.402 ± 0.038

11.036 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.907 ± 0.02

2.76 ± 0.034

5.73 ± 0.042

3.435 ± 0.029

6.995 ± 0.05

5.005 ± 0.028

5.926 ± 0.054

7.273 ± 0.029

1.631 ± 0.025

2.452 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski