Eubacterium oxidoreducens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2618 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6CM44|A0A1G6CM44_EUBOX Uncharacterized protein OS=Eubacterium oxidoreducens OX=1732 GN=SAMN02910417_02510 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.28RR3 pKa = 11.84RR4 pKa = 11.84MKK6 pKa = 10.46RR7 pKa = 11.84IVSLASVVVMGASVFTGCGSSKK29 pKa = 11.12EE30 pKa = 4.2EE31 pKa = 3.98TTQTGSSAEE40 pKa = 3.95QSSAAEE46 pKa = 4.3LSSEE50 pKa = 4.98DD51 pKa = 3.6IIKK54 pKa = 10.52AAAEE58 pKa = 4.06NGSVGNWGLGNEE70 pKa = 4.3YY71 pKa = 10.39EE72 pKa = 4.46VLALLSKK79 pKa = 10.97YY80 pKa = 10.67DD81 pKa = 3.88LGSDD85 pKa = 3.77YY86 pKa = 11.03LSQDD90 pKa = 2.82FTMDD94 pKa = 3.36GFDD97 pKa = 5.02DD98 pKa = 5.31DD99 pKa = 5.93SITLASAMTYY109 pKa = 10.84NEE111 pKa = 4.36LGLVKK116 pKa = 10.28NDD118 pKa = 3.18YY119 pKa = 11.03DD120 pKa = 3.47GGYY123 pKa = 10.63GYY125 pKa = 10.8GDD127 pKa = 3.51SVEE130 pKa = 4.4YY131 pKa = 10.33IDD133 pKa = 5.39MNDD136 pKa = 3.34EE137 pKa = 4.01GVAMLEE143 pKa = 4.11DD144 pKa = 4.68NIFCTEE150 pKa = 4.27DD151 pKa = 3.29FAKK154 pKa = 10.63EE155 pKa = 4.09NPNTVAAFLYY165 pKa = 10.6ASLKK169 pKa = 7.59GWEE172 pKa = 4.22YY173 pKa = 10.94AVEE176 pKa = 4.21NPEE179 pKa = 3.81EE180 pKa = 4.04AAEE183 pKa = 3.87ICYY186 pKa = 9.88TYY188 pKa = 10.76GSSVSAEE195 pKa = 3.95HH196 pKa = 5.32QAYY199 pKa = 8.38MAEE202 pKa = 4.24EE203 pKa = 4.2VAKK206 pKa = 10.7LVTTDD211 pKa = 3.47TQGEE215 pKa = 4.66TVTDD219 pKa = 3.75IGNMDD224 pKa = 4.43EE225 pKa = 6.17DD226 pKa = 5.18AMQQTLDD233 pKa = 3.23IAKK236 pKa = 9.85EE237 pKa = 4.05YY238 pKa = 10.61ISLDD242 pKa = 4.06DD243 pKa = 4.14SDD245 pKa = 5.8ADD247 pKa = 4.03AALQSLTLDD256 pKa = 4.84DD257 pKa = 4.94IRR259 pKa = 11.84DD260 pKa = 3.6SSYY263 pKa = 11.73LEE265 pKa = 3.95AAKK268 pKa = 10.86SSDD271 pKa = 2.98GTFDD275 pKa = 3.51VEE277 pKa = 4.1KK278 pKa = 10.94SEE280 pKa = 5.57VSIQLKK286 pKa = 9.02WLPDD290 pKa = 3.64AQFMGYY296 pKa = 9.62YY297 pKa = 9.43VALDD301 pKa = 3.0KK302 pKa = 11.18GYY304 pKa = 9.73YY305 pKa = 9.51KK306 pKa = 10.55EE307 pKa = 4.22VGLDD311 pKa = 3.42VEE313 pKa = 4.92IVAGGGDD320 pKa = 3.38IAEE323 pKa = 4.37TTAVNNGTVDD333 pKa = 5.1FGVTWVSNLASANAGGMNLVEE354 pKa = 4.31VAQIFQRR361 pKa = 11.84SGLVLVYY368 pKa = 10.49KK369 pKa = 10.28PEE371 pKa = 4.0NFEE374 pKa = 4.0

Molecular weight: 40.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6CQ60|A0A1G6CQ60_EUBOX HD-GYP domain c-di-GMP phosphodiesterase class II (Or its inactivated variant) OS=Eubacterium oxidoreducens OX=1732 GN=SAMN02910417_02581 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.55MTYY5 pKa = 8.41QPKK8 pKa = 8.94RR9 pKa = 11.84RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.85VHH16 pKa = 6.01GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.9VIAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.55GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2618

0

2618

873114

39

6892

333.5

37.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.384 ± 0.046

1.423 ± 0.019

5.966 ± 0.047

7.83 ± 0.051

3.816 ± 0.036

6.717 ± 0.044

1.72 ± 0.022

7.576 ± 0.042

7.257 ± 0.053

8.485 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.07 ± 0.028

4.523 ± 0.036

2.9 ± 0.028

3.328 ± 0.027

3.907 ± 0.046

6.168 ± 0.06

5.759 ± 0.081

6.988 ± 0.041

0.764 ± 0.015

4.416 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski