Acinetobacter phage vB_AbaS_TRS1
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B1P9F5|A0A1B1P9F5_9CAUD Putative class I holin OS=Acinetobacter phage vB_AbaS_TRS1 OX=1852629 GN=TRS1_31 PE=4 SV=1
MM1 pKa = 7.78 ADD3 pKa = 3.6 KK4 pKa = 11.04 KK5 pKa = 10.57 PDD7 pKa = 3.45 YY8 pKa = 10.12 KK9 pKa = 10.93 YY10 pKa = 10.57 QYY12 pKa = 8.31 PTDD15 pKa = 3.3 RR16 pKa = 11.84 RR17 pKa = 11.84 YY18 pKa = 10.94 ADD20 pKa = 4.25 DD21 pKa = 3.98 ATDD24 pKa = 3.46 TLAAGTMFDD33 pKa = 3.73 PAKK36 pKa = 9.9 TAGDD40 pKa = 3.81 YY41 pKa = 11.3 GITDD45 pKa = 3.69 PQVAVPLPEE54 pKa = 4.31 EE55 pKa = 4.33 PTDD58 pKa = 4.01 GGGSAA63 pKa = 4.71
Molecular weight: 6.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.158
IPC2_protein 4.024
IPC_protein 3.973
Toseland 3.732
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.973
Rodwell 3.795
Grimsley 3.656
Solomon 3.973
Lehninger 3.923
Nozaki 4.113
DTASelect 4.406
Thurlkill 3.821
EMBOSS 3.973
Sillero 4.101
Patrickios 3.465
IPC_peptide 3.961
IPC2_peptide 4.062
IPC2.peptide.svr19 4.021
Protein with the highest isoelectric point:
>tr|A0A1B1P9I2|A0A1B1P9I2_9CAUD Putative DNA replication initator protein O OS=Acinetobacter phage vB_AbaS_TRS1 OX=1852629 GN=TRS1_52 PE=4 SV=1
MM1 pKa = 7.48 IEE3 pKa = 4.26 SMTPSMYY10 pKa = 10.25 IKK12 pKa = 10.17 QDD14 pKa = 3.52 FSAEE18 pKa = 3.22 IAAWVEE24 pKa = 3.81 RR25 pKa = 11.84 GNQITVLGRR34 pKa = 11.84 GEE36 pKa = 4.25 STHH39 pKa = 6.37 NKK41 pKa = 9.44 AFNNATKK48 pKa = 9.08 KK49 pKa = 8.95 TPQDD53 pKa = 2.68 AMRR56 pKa = 11.84 RR57 pKa = 11.84 VMANSVAQTRR67 pKa = 11.84 NAKK70 pKa = 10.25 DD71 pKa = 3.36 PYY73 pKa = 9.81 FQVRR77 pKa = 11.84 LKK79 pKa = 10.86 AKK81 pKa = 10.49 QEE83 pKa = 3.82 GQLYY87 pKa = 10.14 FDD89 pKa = 4.62 GKK91 pKa = 11.08 ACIKK95 pKa = 10.47 CKK97 pKa = 7.64 STKK100 pKa = 10.15 RR101 pKa = 11.84 YY102 pKa = 9.61 VSSNKK107 pKa = 9.54 CLHH110 pKa = 6.46 CVNEE114 pKa = 4.13 SNRR117 pKa = 11.84 RR118 pKa = 11.84 YY119 pKa = 9.81 KK120 pKa = 10.84 EE121 pKa = 3.71 RR122 pKa = 11.84 MAA124 pKa = 5.55
Molecular weight: 14.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.121
IPC2_protein 9.209
IPC_protein 9.18
Toseland 9.999
ProMoST 9.633
Dawson 10.175
Bjellqvist 9.853
Wikipedia 10.321
Rodwell 10.672
Grimsley 10.233
Solomon 10.204
Lehninger 10.189
Nozaki 10.058
DTASelect 9.823
Thurlkill 10.043
EMBOSS 10.394
Sillero 10.101
Patrickios 10.35
IPC_peptide 10.204
IPC2_peptide 8.609
IPC2.peptide.svr19 8.12
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
70
0
70
12780
39
944
182.6
20.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.879 ± 0.446
1.166 ± 0.137
5.696 ± 0.228
7.152 ± 0.299
3.975 ± 0.205
6.299 ± 0.334
1.87 ± 0.166
6.228 ± 0.247
7.535 ± 0.391
8.615 ± 0.282
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.48 ± 0.152
5.36 ± 0.159
3.161 ± 0.236
4.523 ± 0.266
4.499 ± 0.241
6.299 ± 0.272
6.228 ± 0.411
6.103 ± 0.256
1.244 ± 0.101
3.685 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here