Acinetobacter phage vB_AbaS_TRS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vieuvirus; unclassified Vieuvirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1P9F5|A0A1B1P9F5_9CAUD Putative class I holin OS=Acinetobacter phage vB_AbaS_TRS1 OX=1852629 GN=TRS1_31 PE=4 SV=1
MM1 pKa = 7.78ADD3 pKa = 3.6KK4 pKa = 11.04KK5 pKa = 10.57PDD7 pKa = 3.45YY8 pKa = 10.12KK9 pKa = 10.93YY10 pKa = 10.57QYY12 pKa = 8.31PTDD15 pKa = 3.3RR16 pKa = 11.84RR17 pKa = 11.84YY18 pKa = 10.94ADD20 pKa = 4.25DD21 pKa = 3.98ATDD24 pKa = 3.46TLAAGTMFDD33 pKa = 3.73PAKK36 pKa = 9.9TAGDD40 pKa = 3.81YY41 pKa = 11.3GITDD45 pKa = 3.69PQVAVPLPEE54 pKa = 4.31EE55 pKa = 4.33PTDD58 pKa = 4.01GGGSAA63 pKa = 4.71

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1P9I2|A0A1B1P9I2_9CAUD Putative DNA replication initator protein O OS=Acinetobacter phage vB_AbaS_TRS1 OX=1852629 GN=TRS1_52 PE=4 SV=1
MM1 pKa = 7.48IEE3 pKa = 4.26SMTPSMYY10 pKa = 10.25IKK12 pKa = 10.17QDD14 pKa = 3.52FSAEE18 pKa = 3.22IAAWVEE24 pKa = 3.81RR25 pKa = 11.84GNQITVLGRR34 pKa = 11.84GEE36 pKa = 4.25STHH39 pKa = 6.37NKK41 pKa = 9.44AFNNATKK48 pKa = 9.08KK49 pKa = 8.95TPQDD53 pKa = 2.68AMRR56 pKa = 11.84RR57 pKa = 11.84VMANSVAQTRR67 pKa = 11.84NAKK70 pKa = 10.25DD71 pKa = 3.36PYY73 pKa = 9.81FQVRR77 pKa = 11.84LKK79 pKa = 10.86AKK81 pKa = 10.49QEE83 pKa = 3.82GQLYY87 pKa = 10.14FDD89 pKa = 4.62GKK91 pKa = 11.08ACIKK95 pKa = 10.47CKK97 pKa = 7.64STKK100 pKa = 10.15RR101 pKa = 11.84YY102 pKa = 9.61VSSNKK107 pKa = 9.54CLHH110 pKa = 6.46CVNEE114 pKa = 4.13SNRR117 pKa = 11.84RR118 pKa = 11.84YY119 pKa = 9.81KK120 pKa = 10.84EE121 pKa = 3.71RR122 pKa = 11.84MAA124 pKa = 5.55

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

12780

39

944

182.6

20.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.879 ± 0.446

1.166 ± 0.137

5.696 ± 0.228

7.152 ± 0.299

3.975 ± 0.205

6.299 ± 0.334

1.87 ± 0.166

6.228 ± 0.247

7.535 ± 0.391

8.615 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.48 ± 0.152

5.36 ± 0.159

3.161 ± 0.236

4.523 ± 0.266

4.499 ± 0.241

6.299 ± 0.272

6.228 ± 0.411

6.103 ± 0.256

1.244 ± 0.101

3.685 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski