Pseudomonas sp. URIL14HWK12:I9

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4431 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A285NV48|A0A285NV48_9PSED Uncharacterized conserved protein OS=Pseudomonas sp. URIL14HWK12:I9 OX=1261633 GN=SAMN05660463_02201 PE=4 SV=1
MM1 pKa = 7.69NSICRR6 pKa = 11.84DD7 pKa = 3.09TAYY10 pKa = 9.86WRR12 pKa = 11.84TRR14 pKa = 11.84LSSLLEE20 pKa = 3.68QEE22 pKa = 5.19LRR24 pKa = 11.84MEE26 pKa = 5.28PGTLACEE33 pKa = 4.36RR34 pKa = 11.84NLSEE38 pKa = 4.61YY39 pKa = 11.04GLDD42 pKa = 3.66SIVALTLVGDD52 pKa = 4.52LEE54 pKa = 5.72DD55 pKa = 3.93EE56 pKa = 4.6LGMTLEE62 pKa = 4.44PTLVWDD68 pKa = 4.23YY69 pKa = 11.37PSVDD73 pKa = 3.25ALAAYY78 pKa = 8.55LSCLAEE84 pKa = 4.09PAQPSIALAADD95 pKa = 3.68APGLAA100 pKa = 4.55

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A285PC76|A0A285PC76_9PSED RecBCD enzyme subunit RecB OS=Pseudomonas sp. URIL14HWK12:I9 OX=1261633 GN=recB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.28NGRR28 pKa = 11.84TVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.98GRR39 pKa = 11.84KK40 pKa = 8.8RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4431

0

4431

1472299

26

3742

332.3

36.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.184 ± 0.047

1.013 ± 0.012

5.119 ± 0.028

5.482 ± 0.037

3.462 ± 0.024

8.281 ± 0.04

2.357 ± 0.021

4.302 ± 0.027

2.998 ± 0.028

11.798 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.017

2.796 ± 0.024

5.079 ± 0.027

4.794 ± 0.034

6.655 ± 0.041

5.465 ± 0.03

4.811 ± 0.039

7.238 ± 0.029

1.467 ± 0.017

2.439 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski