Amino acid dipepetide frequency for Pseudomonas sp. URIL14HWK12:I9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.323AlaAla: 14.323 ± 0.148	1.428AlaCys: 1.428 ± 0.032	5.989AlaAsp: 5.989 ± 0.075	6.919AlaGlu: 6.919 ± 0.081	4.001AlaPhe: 4.001 ± 0.052	10.403AlaGly: 10.403 ± 0.092	2.669AlaHis: 2.669 ± 0.043	4.966AlaIle: 4.966 ± 0.058	3.406AlaLys: 3.406 ± 0.061	15.882AlaLeu: 15.882 ± 0.142	3.111AlaMet: 3.111 ± 0.053	3.154AlaAsn: 3.154 ± 0.053	5.652AlaPro: 5.652 ± 0.093	6.594AlaGln: 6.594 ± 0.084	8.256AlaArg: 8.256 ± 0.093	6.956AlaSer: 6.956 ± 0.074	5.402AlaThr: 5.402 ± 0.078	8.122AlaVal: 8.122 ± 0.093	1.987AlaTrp: 1.987 ± 0.042	2.614AlaTyr: 2.614 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	1.21CysAla: 1.21 ± 0.029	0.142CysCys: 0.142 ± 0.009	0.571CysAsp: 0.571 ± 0.018	0.586CysGlu: 0.586 ± 0.02	0.361CysPhe: 0.361 ± 0.014	0.92CysGly: 0.92 ± 0.026	0.251CysHis: 0.251 ± 0.013	0.418CysIle: 0.418 ± 0.017	0.231CysLys: 0.231 ± 0.012	1.224CysLeu: 1.224 ± 0.029	0.203CysMet: 0.203 ± 0.013	0.272CysAsn: 0.272 ± 0.013	0.505CysPro: 0.505 ± 0.021	0.446CysGln: 0.446 ± 0.019	0.621CysArg: 0.621 ± 0.023	0.571CysSer: 0.571 ± 0.019	0.49CysThr: 0.49 ± 0.017	0.702CysVal: 0.702 ± 0.024	0.158CysTrp: 0.158 ± 0.011	0.253CysTyr: 0.253 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.011AspAla: 6.011 ± 0.069	0.49AspCys: 0.49 ± 0.018	2.742AspAsp: 2.742 ± 0.047	3.173AspGlu: 3.173 ± 0.047	1.95AspPhe: 1.95 ± 0.041	4.284AspGly: 4.284 ± 0.065	1.244AspHis: 1.244 ± 0.03	2.438AspIle: 2.438 ± 0.04	1.553AspLys: 1.553 ± 0.037	5.526AspLeu: 5.526 ± 0.072	1.068AspMet: 1.068 ± 0.025	1.433AspAsn: 1.433 ± 0.035	2.792AspPro: 2.792 ± 0.053	2.245AspGln: 2.245 ± 0.044	3.153AspArg: 3.153 ± 0.056	2.627AspSer: 2.627 ± 0.049	2.575AspThr: 2.575 ± 0.047	3.431AspVal: 3.431 ± 0.051	0.829AspTrp: 0.829 ± 0.022	1.625AspTyr: 1.625 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	6.846GluAla: 6.846 ± 0.082	0.409GluCys: 0.409 ± 0.016	2.182GluAsp: 2.182 ± 0.037	2.642GluGlu: 2.642 ± 0.057	1.534GluPhe: 1.534 ± 0.033	4.261GluGly: 4.261 ± 0.064	1.814GluHis: 1.814 ± 0.041	2.267GluIle: 2.267 ± 0.043	1.775GluLys: 1.775 ± 0.042	6.419GluLeu: 6.419 ± 0.082	1.181GluMet: 1.181 ± 0.031	1.353GluAsn: 1.353 ± 0.031	2.828GluPro: 2.828 ± 0.053	3.988GluGln: 3.988 ± 0.063	4.911GluArg: 4.911 ± 0.064	2.222GluSer: 2.222 ± 0.048	2.405GluThr: 2.405 ± 0.039	4.133GluVal: 4.133 ± 0.058	0.616GluTrp: 0.616 ± 0.018	1.039GluTyr: 1.039 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	3.869PheAla: 3.869 ± 0.046	0.446PheCys: 0.446 ± 0.019	2.451PheAsp: 2.451 ± 0.043	2.18PheGlu: 2.18 ± 0.044	1.341PhePhe: 1.341 ± 0.039	3.201PheGly: 3.201 ± 0.054	0.714PheHis: 0.714 ± 0.023	1.667PheIle: 1.667 ± 0.037	1.182PheLys: 1.182 ± 0.034	2.996PheLeu: 2.996 ± 0.053	0.793PheMet: 0.793 ± 0.023	1.271PheAsn: 1.271 ± 0.03	1.364PhePro: 1.364 ± 0.032	1.158PheGln: 1.158 ± 0.032	1.717PheArg: 1.717 ± 0.036	2.416PheSer: 2.416 ± 0.041	2.013PheThr: 2.013 ± 0.043	2.377PheVal: 2.377 ± 0.043	0.511PheTrp: 0.511 ± 0.02	0.954PheTyr: 0.954 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	8.3GlyAla: 8.3 ± 0.092	0.957GlyCys: 0.957 ± 0.028	3.961GlyAsp: 3.961 ± 0.065	4.815GlyGlu: 4.815 ± 0.064	3.362GlyPhe: 3.362 ± 0.055	6.322GlyGly: 6.322 ± 0.091	2.063GlyHis: 2.063 ± 0.038	3.861GlyIle: 3.861 ± 0.054	3.142GlyLys: 3.142 ± 0.048	9.752GlyLeu: 9.752 ± 0.096	2.222GlyMet: 2.222 ± 0.039	2.442GlyAsn: 2.442 ± 0.06	2.984GlyPro: 2.984 ± 0.054	4.326GlyGln: 4.326 ± 0.058	5.226GlyArg: 5.226 ± 0.077	4.606GlySer: 4.606 ± 0.063	4.218GlyThr: 4.218 ± 0.073	6.273GlyVal: 6.273 ± 0.063	1.464GlyTrp: 1.464 ± 0.034	2.514GlyTyr: 2.514 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.593HisAla: 2.593 ± 0.045	0.354HisCys: 0.354 ± 0.016	1.225HisAsp: 1.225 ± 0.035	1.173HisGlu: 1.173 ± 0.025	0.958HisPhe: 0.958 ± 0.027	2.133HisGly: 2.133 ± 0.04	0.638HisHis: 0.638 ± 0.024	0.923HisIle: 0.923 ± 0.025	0.584HisLys: 0.584 ± 0.019	2.745HisLeu: 2.745 ± 0.048	0.547HisMet: 0.547 ± 0.021	0.628HisAsn: 0.628 ± 0.023	1.491HisPro: 1.491 ± 0.03	1.024HisGln: 1.024 ± 0.028	1.475HisArg: 1.475 ± 0.031	1.301HisSer: 1.301 ± 0.028	1.106HisThr: 1.106 ± 0.029	1.424HisVal: 1.424 ± 0.03	0.505HisTrp: 0.505 ± 0.021	0.746HisTyr: 0.746 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.68IleAla: 5.68 ± 0.066	0.447IleCys: 0.447 ± 0.017	3.108IleAsp: 3.108 ± 0.048	3.03IleGlu: 3.03 ± 0.046	1.222IlePhe: 1.222 ± 0.03	4.204IleGly: 4.204 ± 0.054	0.876IleHis: 0.876 ± 0.026	1.776IleIle: 1.776 ± 0.035	1.43IleLys: 1.43 ± 0.038	3.529IleLeu: 3.529 ± 0.056	0.7IleMet: 0.7 ± 0.022	1.486IleAsn: 1.486 ± 0.031	1.951IlePro: 1.951 ± 0.036	1.421IleGln: 1.421 ± 0.029	2.629IleArg: 2.629 ± 0.042	2.56IleSer: 2.56 ± 0.045	2.578IleThr: 2.578 ± 0.039	2.993IleVal: 2.993 ± 0.048	0.425IleTrp: 0.425 ± 0.019	0.971IleTyr: 0.971 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	4.254LysAla: 4.254 ± 0.074	0.168LysCys: 0.168 ± 0.012	1.453LysAsp: 1.453 ± 0.038	1.282LysGlu: 1.282 ± 0.036	0.734LysPhe: 0.734 ± 0.025	2.511LysGly: 2.511 ± 0.041	0.648LysHis: 0.648 ± 0.02	1.248LysIle: 1.248 ± 0.034	1.082LysLys: 1.082 ± 0.038	3.266LysLeu: 3.266 ± 0.051	0.591LysMet: 0.591 ± 0.02	0.839LysAsn: 0.839 ± 0.024	1.941LysPro: 1.941 ± 0.044	1.373LysGln: 1.373 ± 0.033	2.153LysArg: 2.153 ± 0.041	1.453LysSer: 1.453 ± 0.038	1.463LysThr: 1.463 ± 0.038	2.639LysVal: 2.639 ± 0.045	0.312LysTrp: 0.312 ± 0.013	0.568LysTyr: 0.568 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	16.528LeuAla: 16.528 ± 0.155	1.338LeuCys: 1.338 ± 0.034	6.321LeuAsp: 6.321 ± 0.079	6.452LeuGlu: 6.452 ± 0.089	3.933LeuPhe: 3.933 ± 0.056	9.946LeuGly: 9.946 ± 0.101	2.512LeuHis: 2.512 ± 0.041	5.085LeuIle: 5.085 ± 0.064	3.903LeuLys: 3.903 ± 0.056	12.9LeuLeu: 12.9 ± 0.146	2.62LeuMet: 2.62 ± 0.045	3.421LeuAsn: 3.421 ± 0.056	6.575LeuPro: 6.575 ± 0.081	4.639LeuGln: 4.639 ± 0.069	7.7LeuArg: 7.7 ± 0.09	6.788LeuSer: 6.788 ± 0.068	5.332LeuThr: 5.332 ± 0.071	8.009LeuVal: 8.009 ± 0.077	1.454LeuTrp: 1.454 ± 0.041	2.523LeuTyr: 2.523 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	2.97MetAla: 2.97 ± 0.048	0.17MetCys: 0.17 ± 0.011	1.005MetAsp: 1.005 ± 0.027	0.939MetGlu: 0.939 ± 0.026	0.637MetPhe: 0.637 ± 0.022	1.743MetGly: 1.743 ± 0.037	0.49MetHis: 0.49 ± 0.021	1.095MetIle: 1.095 ± 0.026	0.795MetLys: 0.795 ± 0.023	2.585MetLeu: 2.585 ± 0.041	0.532MetMet: 0.532 ± 0.021	0.817MetAsn: 0.817 ± 0.023	1.414MetPro: 1.414 ± 0.036	1.024MetGln: 1.024 ± 0.026	1.469MetArg: 1.469 ± 0.038	1.5MetSer: 1.5 ± 0.028	1.333MetThr: 1.333 ± 0.03	1.572MetVal: 1.572 ± 0.038	0.174MetTrp: 0.174 ± 0.011	0.335MetTyr: 0.335 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.34AsnAla: 3.34 ± 0.051	0.247AsnCys: 0.247 ± 0.013	1.496AsnAsp: 1.496 ± 0.034	1.381AsnGlu: 1.381 ± 0.027	0.931AsnPhe: 0.931 ± 0.026	2.483AsnGly: 2.483 ± 0.049	0.636AsnHis: 0.636 ± 0.02	1.253AsnIle: 1.253 ± 0.032	0.743AsnLys: 0.743 ± 0.025	3.312AsnLeu: 3.312 ± 0.051	0.553AsnMet: 0.553 ± 0.019	0.824AsnAsn: 0.824 ± 0.024	1.813AsnPro: 1.813 ± 0.038	1.183AsnGln: 1.183 ± 0.025	1.78AsnArg: 1.78 ± 0.037	1.426AsnSer: 1.426 ± 0.034	1.48AsnThr: 1.48 ± 0.037	1.97AsnVal: 1.97 ± 0.038	0.39AsnTrp: 0.39 ± 0.015	0.72AsnTyr: 0.72 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	6.815ProAla: 6.815 ± 0.093	0.447ProCys: 0.447 ± 0.017	2.703ProAsp: 2.703 ± 0.049	3.247ProGlu: 3.247 ± 0.043	1.788ProPhe: 1.788 ± 0.04	4.522ProGly: 4.522 ± 0.065	1.138ProHis: 1.138 ± 0.029	1.904ProIle: 1.904 ± 0.043	1.401ProLys: 1.401 ± 0.037	5.855ProLeu: 5.855 ± 0.073	1.269ProMet: 1.269 ± 0.028	1.3ProAsn: 1.3 ± 0.032	2.248ProPro: 2.248 ± 0.045	2.244ProGln: 2.244 ± 0.035	2.796ProArg: 2.796 ± 0.049	2.762ProSer: 2.762 ± 0.052	2.301ProThr: 2.301 ± 0.04	3.997ProVal: 3.997 ± 0.055	0.859ProTrp: 0.859 ± 0.028	1.199ProTyr: 1.199 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	7.768GlnAla: 7.768 ± 0.1	0.369GlnCys: 0.369 ± 0.014	1.682GlnAsp: 1.682 ± 0.037	1.808GlnGlu: 1.808 ± 0.035	1.301GlnPhe: 1.301 ± 0.03	4.051GlnGly: 4.051 ± 0.058	1.132GlnHis: 1.132 ± 0.029	1.807GlnIle: 1.807 ± 0.033	1.087GlnLys: 1.087 ± 0.03	5.504GlnLeu: 5.504 ± 0.061	1.073GlnMet: 1.073 ± 0.023	1.019GlnAsn: 1.019 ± 0.024	2.682GlnPro: 2.682 ± 0.045	2.682GlnGln: 2.682 ± 0.057	3.838GlnArg: 3.838 ± 0.064	2.111GlnSer: 2.111 ± 0.043	1.799GlnThr: 1.799 ± 0.038	4.199GlnVal: 4.199 ± 0.046	0.957GlnTrp: 0.957 ± 0.026	1.064GlnTyr: 1.064 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	6.887ArgAla: 6.887 ± 0.082	0.677ArgCys: 0.677 ± 0.021	3.315ArgAsp: 3.315 ± 0.049	4.171ArgGlu: 4.171 ± 0.067	2.762ArgPhe: 2.762 ± 0.051	4.21ArgGly: 4.21 ± 0.069	1.83ArgHis: 1.83 ± 0.038	3.085ArgIle: 3.085 ± 0.044	1.953ArgLys: 1.953 ± 0.034	8.879ArgLeu: 8.879 ± 0.096	1.625ArgMet: 1.625 ± 0.035	1.827ArgAsn: 1.827 ± 0.034	3.037ArgPro: 3.037 ± 0.052	3.814ArgGln: 3.814 ± 0.055	4.508ArgArg: 4.508 ± 0.069	3.281ArgSer: 3.281 ± 0.044	2.836ArgThr: 2.836 ± 0.048	4.595ArgVal: 4.595 ± 0.06	1.174ArgTrp: 1.174 ± 0.033	2.087ArgTyr: 2.087 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	6.403SerAla: 6.403 ± 0.083	0.414SerCys: 0.414 ± 0.019	2.798SerAsp: 2.798 ± 0.046	2.828SerGlu: 2.828 ± 0.047	2.028SerPhe: 2.028 ± 0.034	4.96SerGly: 4.96 ± 0.072	1.266SerHis: 1.266 ± 0.03	2.389SerIle: 2.389 ± 0.046	1.539SerLys: 1.539 ± 0.041	6.804SerLeu: 6.804 ± 0.079	1.231SerMet: 1.231 ± 0.031	1.535SerAsn: 1.535 ± 0.034	2.745SerPro: 2.745 ± 0.051	2.436SerGln: 2.436 ± 0.043	3.382SerArg: 3.382 ± 0.05	3.095SerSer: 3.095 ± 0.061	2.838SerThr: 2.838 ± 0.046	3.894SerVal: 3.894 ± 0.059	0.704SerTrp: 0.704 ± 0.021	1.362SerTyr: 1.362 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	5.411ThrAla: 5.411 ± 0.078	0.452ThrCys: 0.452 ± 0.019	2.505ThrAsp: 2.505 ± 0.046	2.28ThrGlu: 2.28 ± 0.041	1.801ThrPhe: 1.801 ± 0.038	4.285ThrGly: 4.285 ± 0.066	1.108ThrHis: 1.108 ± 0.027	1.68ThrIle: 1.68 ± 0.036	0.926ThrLys: 0.926 ± 0.029	6.98ThrLeu: 6.98 ± 0.082	0.742ThrMet: 0.742 ± 0.025	1.079ThrAsn: 1.079 ± 0.031	3.111ThrPro: 3.111 ± 0.05	2.195ThrGln: 2.195 ± 0.042	3.042ThrArg: 3.042 ± 0.046	2.547ThrSer: 2.547 ± 0.046	2.363ThrThr: 2.363 ± 0.053	3.678ThrVal: 3.678 ± 0.065	0.725ThrTrp: 0.725 ± 0.021	1.195ThrTyr: 1.195 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	8.546ValAla: 8.546 ± 0.079	0.732ValCys: 0.732 ± 0.024	3.818ValAsp: 3.818 ± 0.047	4.174ValGlu: 4.174 ± 0.062	2.492ValPhe: 2.492 ± 0.048	5.42ValGly: 5.42 ± 0.061	1.558ValHis: 1.558 ± 0.032	3.657ValIle: 3.657 ± 0.056	2.186ValLys: 2.186 ± 0.047	8.458ValLeu: 8.458 ± 0.092	1.773ValMet: 1.773 ± 0.036	2.149ValAsn: 2.149 ± 0.037	3.625ValPro: 3.625 ± 0.053	3.0ValGln: 3.0 ± 0.049	4.653ValArg: 4.653 ± 0.057	4.237ValSer: 4.237 ± 0.056	3.833ValThr: 3.833 ± 0.067	5.701ValVal: 5.701 ± 0.081	0.796ValTrp: 0.796 ± 0.023	1.576ValTyr: 1.576 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.56TrpAla: 1.56 ± 0.034	0.166TrpCys: 0.166 ± 0.011	0.564TrpAsp: 0.564 ± 0.02	0.595TrpGlu: 0.595 ± 0.022	0.554TrpPhe: 0.554 ± 0.02	0.933TrpGly: 0.933 ± 0.025	0.427TrpHis: 0.427 ± 0.018	0.556TrpIle: 0.556 ± 0.021	0.395TrpLys: 0.395 ± 0.015	2.389TrpLeu: 2.389 ± 0.051	0.327TrpMet: 0.327 ± 0.016	0.404TrpAsn: 0.404 ± 0.017	0.755TrpPro: 0.755 ± 0.022	0.926TrpGln: 0.926 ± 0.028	1.228TrpArg: 1.228 ± 0.035	0.751TrpSer: 0.751 ± 0.022	0.462TrpThr: 0.462 ± 0.019	1.091TrpVal: 1.091 ± 0.028	0.242TrpTrp: 0.242 ± 0.013	0.347TrpTyr: 0.347 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.523TyrAla: 2.523 ± 0.048	0.281TyrCys: 0.281 ± 0.014	1.301TyrAsp: 1.301 ± 0.036	1.149TyrGlu: 1.149 ± 0.031	0.931TyrPhe: 0.931 ± 0.025	2.019TyrGly: 2.019 ± 0.041	0.557TyrHis: 0.557 ± 0.02	0.939TyrIle: 0.939 ± 0.024	0.666TyrLys: 0.666 ± 0.024	2.976TyrLeu: 2.976 ± 0.045	0.435TyrMet: 0.435 ± 0.017	0.706TyrAsn: 0.706 ± 0.022	1.282TyrPro: 1.282 ± 0.029	1.219TyrGln: 1.219 ± 0.032	2.013TyrArg: 2.013 ± 0.043	1.443TyrSer: 1.443 ± 0.034	1.278TyrThr: 1.278 ± 0.031	1.586TyrVal: 1.586 ± 0.036	0.389TyrTrp: 0.389 ± 0.015	0.697TyrTyr: 0.697 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4431 proteins (1472299 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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