Streptococcus phage Javan377

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A9S8|A0A4D6A9S8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan377 OX=2548135 GN=Javan377_0011 PE=4 SV=1
MM1 pKa = 7.15SQVLKK6 pKa = 10.43EE7 pKa = 3.91AGINIIGLPSTVTLGQQLANNGFYY31 pKa = 10.21RR32 pKa = 11.84VSINQDD38 pKa = 2.07WDD40 pKa = 3.54AQPADD45 pKa = 4.57IILMSWGADD54 pKa = 3.06MSTSGGDD61 pKa = 3.5GGHH64 pKa = 6.08VGAMIDD70 pKa = 3.28DD71 pKa = 4.65TYY73 pKa = 11.13FISCDD78 pKa = 3.41YY79 pKa = 9.38STQGATGQAINTYY92 pKa = 8.88PWNDD96 pKa = 3.24YY97 pKa = 10.81YY98 pKa = 11.66GWNKK102 pKa = 8.83PAYY105 pKa = 8.57IEE107 pKa = 3.88VWRR110 pKa = 11.84YY111 pKa = 10.53ADD113 pKa = 3.87TAPQTDD119 pKa = 2.99NRR121 pKa = 11.84ANTTVQPQDD130 pKa = 2.98KK131 pKa = 10.2AYY133 pKa = 10.97YY134 pKa = 8.15EE135 pKa = 4.31ANEE138 pKa = 3.96VKK140 pKa = 10.25YY141 pKa = 11.21VNGIWQIKK149 pKa = 9.1CDD151 pKa = 3.74YY152 pKa = 9.01LAPVGFDD159 pKa = 2.79WTEE162 pKa = 3.54NGIPVSMVNWVDD174 pKa = 3.19KK175 pKa = 11.14DD176 pKa = 3.99GNNLPDD182 pKa = 4.54GADD185 pKa = 3.45KK186 pKa = 11.0DD187 pKa = 4.54FKK189 pKa = 11.3SGMFFSFEE197 pKa = 4.12LDD199 pKa = 3.27EE200 pKa = 4.71AHH202 pKa = 6.75ITDD205 pKa = 3.86TGKK208 pKa = 10.07GGYY211 pKa = 9.11YY212 pKa = 10.14GGYY215 pKa = 7.62YY216 pKa = 8.88WRR218 pKa = 11.84LFEE221 pKa = 5.13FGQFGPIWLSCWDD234 pKa = 3.98KK235 pKa = 11.8DD236 pKa = 4.5DD237 pKa = 4.37LVNYY241 pKa = 9.92YY242 pKa = 9.54EE243 pKa = 4.09

Molecular weight: 27.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A8Q1|A0A4D6A8Q1_9CAUD YopX domain-containing protein OS=Streptococcus phage Javan377 OX=2548135 GN=Javan377_0026 PE=4 SV=1
MM1 pKa = 7.12TRR3 pKa = 11.84SKK5 pKa = 10.9NVEE8 pKa = 3.94GVALEE13 pKa = 4.42LFMSKK18 pKa = 10.51NSVYY22 pKa = 10.78RR23 pKa = 11.84NVIKK27 pKa = 10.5PFFEE31 pKa = 4.32GLEE34 pKa = 4.19KK35 pKa = 10.63KK36 pKa = 8.94VTSIFLKK43 pKa = 10.1TRR45 pKa = 3.31

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

11505

37

1566

239.7

26.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.849 ± 0.965

0.461 ± 0.101

5.858 ± 0.449

8.249 ± 0.805

3.998 ± 0.201

6.771 ± 0.355

1.26 ± 0.209

6.319 ± 0.295

7.997 ± 0.459

8.509 ± 0.332

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.46 ± 0.226

5.528 ± 0.363

2.92 ± 0.337

4.763 ± 0.432

4.337 ± 0.492

5.867 ± 0.757

6.041 ± 0.365

5.989 ± 0.21

1.217 ± 0.196

3.607 ± 0.441
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski