Sulfitobacter phage phiGT1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5DYH5|A0A7D5DYH5_9CAUD Uncharacterized protein OS=Sulfitobacter phage phiGT1 OX=2745703 GN=phiGT1_46 PE=4 SV=1
MM1 pKa = 6.69WTLTVLTGYY10 pKa = 11.08CMGVWCTVDD19 pKa = 3.4YY20 pKa = 11.62NKK22 pKa = 10.7GFPSWDD28 pKa = 3.03ACNAEE33 pKa = 4.04RR34 pKa = 11.84AAIIAADD41 pKa = 4.07NPDD44 pKa = 4.49DD45 pKa = 3.85DD46 pKa = 4.06TVIVVKK52 pKa = 8.7CTPTKK57 pKa = 10.66LL58 pKa = 3.42

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5DW61|A0A7D5DW61_9CAUD Uncharacterized protein OS=Sulfitobacter phage phiGT1 OX=2745703 GN=phiGT1_47 PE=4 SV=1
MM1 pKa = 7.95AIRR4 pKa = 11.84VQGLPRR10 pKa = 11.84PDD12 pKa = 3.39WEE14 pKa = 4.37RR15 pKa = 11.84LRR17 pKa = 11.84ALPQQSLSKK26 pKa = 9.75VLRR29 pKa = 11.84YY30 pKa = 9.54SRR32 pKa = 11.84PLLCGEE38 pKa = 4.71AAQDD42 pKa = 4.57DD43 pKa = 5.28RR44 pKa = 11.84GGQWNAAQDD53 pKa = 4.05GLSEE57 pKa = 4.55FPFISAADD65 pKa = 3.67YY66 pKa = 10.65HH67 pKa = 6.87GKK69 pKa = 9.64NPATSVSKK77 pKa = 10.62DD78 pKa = 3.14RR79 pKa = 11.84RR80 pKa = 11.84DD81 pKa = 3.2ALTINRR87 pKa = 11.84IDD89 pKa = 3.5VVSRR93 pKa = 11.84IWLQWSPLDD102 pKa = 3.95NVKK105 pKa = 9.16RR106 pKa = 11.84TANPSRR112 pKa = 11.84HH113 pKa = 6.1LMSADD118 pKa = 3.13GFNSRR123 pKa = 11.84GFSLLSVQVVAVGAARR139 pKa = 11.84VHH141 pKa = 6.49LFLSHH146 pKa = 6.52TPP148 pKa = 3.42

Molecular weight: 16.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

12684

52

856

183.8

20.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.479 ± 0.369

1.135 ± 0.175

6.638 ± 0.3

5.637 ± 0.385

3.343 ± 0.229

9.177 ± 0.546

1.671 ± 0.239

4.833 ± 0.215

4.951 ± 0.342

7.182 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.964 ± 0.269

4.021 ± 0.247

4.431 ± 0.23

4.257 ± 0.366

6.015 ± 0.354

5.787 ± 0.265

5.929 ± 0.446

6.244 ± 0.264

1.79 ± 0.212

2.515 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski