Pseudomonas mendocina (strain ymp)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas aeruginosa group; Pseudomonas mendocina

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4563 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4XN66|A4XN66_PSEMY Uncharacterized protein OS=Pseudomonas mendocina (strain ymp) OX=399739 GN=Pmen_0007 PE=4 SV=1
MM1 pKa = 7.62TNYY4 pKa = 9.92QQITPFSYY12 pKa = 10.15IPLSISATVNTLLYY26 pKa = 7.54GTKK29 pKa = 9.41WGGGLGFPVTLTYY42 pKa = 11.12SFANPGVSQYY52 pKa = 10.18STDD55 pKa = 3.34YY56 pKa = 11.66DD57 pKa = 3.96DD58 pKa = 6.2FVEE61 pKa = 4.6YY62 pKa = 7.89WTGSNSSNFIYY73 pKa = 10.54SLSSNQEE80 pKa = 3.76SAVRR84 pKa = 11.84SALAQWSSVANLSFTEE100 pKa = 4.21VSDD103 pKa = 3.86TAGGTVGDD111 pKa = 4.48LRR113 pKa = 11.84FGGYY117 pKa = 10.42KK118 pKa = 10.58LIDD121 pKa = 3.26WDD123 pKa = 3.84TAAFAFLPGASPLAGDD139 pKa = 3.52VWIGPVTNEE148 pKa = 4.0VNPSGGSYY156 pKa = 10.7DD157 pKa = 3.84YY158 pKa = 10.89LTFLHH163 pKa = 6.95EE164 pKa = 4.63IGHH167 pKa = 6.36ALGLKK172 pKa = 10.22HH173 pKa = 6.57PFEE176 pKa = 5.92ASGSSNYY183 pKa = 8.99VLRR186 pKa = 11.84GAYY189 pKa = 10.06DD190 pKa = 3.49DD191 pKa = 3.74VRR193 pKa = 11.84YY194 pKa = 7.89TVMSYY199 pKa = 11.34NDD201 pKa = 3.8TYY203 pKa = 11.83DD204 pKa = 3.87FLPTGPMLYY213 pKa = 10.22DD214 pKa = 3.75IAAMQYY220 pKa = 10.05IYY222 pKa = 10.93GSNDD226 pKa = 2.33TWQTGDD232 pKa = 4.69NVYY235 pKa = 10.65SWDD238 pKa = 3.31ANARR242 pKa = 11.84IFEE245 pKa = 4.74TIWDD249 pKa = 3.72AGGNDD254 pKa = 4.31TIDD257 pKa = 4.51ASNQSAGVVINLNSGEE273 pKa = 4.12FSSIGQSIYY282 pKa = 11.16NYY284 pKa = 9.24ATGAGRR290 pKa = 11.84NNFLTIAYY298 pKa = 9.73GSVIEE303 pKa = 4.19NAIGSDD309 pKa = 3.8YY310 pKa = 11.28KK311 pKa = 10.78DD312 pKa = 3.37TLIGNSADD320 pKa = 3.53NVLDD324 pKa = 3.9GGKK327 pKa = 10.65GEE329 pKa = 4.15DD330 pKa = 3.8VLRR333 pKa = 11.84GGAGNDD339 pKa = 3.26TYY341 pKa = 11.7YY342 pKa = 11.33VDD344 pKa = 5.47LPTGSDD350 pKa = 3.33FYY352 pKa = 11.84DD353 pKa = 3.29RR354 pKa = 11.84VTEE357 pKa = 4.11YY358 pKa = 11.04AGEE361 pKa = 4.71GYY363 pKa = 10.78DD364 pKa = 3.86KK365 pKa = 11.21VVLRR369 pKa = 11.84GTSTGFFGRR378 pKa = 11.84SFALANNVEE387 pKa = 4.49EE388 pKa = 4.38VDD390 pKa = 3.62ASQTGAVALTLLGNNSANRR409 pKa = 11.84IIGNAGNNLLNGGAGADD426 pKa = 3.74TMIGGAGNDD435 pKa = 3.83TYY437 pKa = 11.68VVDD440 pKa = 3.83HH441 pKa = 6.98LGDD444 pKa = 3.65EE445 pKa = 4.78VIEE448 pKa = 4.14QVGEE452 pKa = 4.46GIDD455 pKa = 3.71LVQVAIATAGGTYY468 pKa = 9.85TLGDD472 pKa = 3.71HH473 pKa = 6.67VEE475 pKa = 4.25NATLTNSVSFNLVGNTLSNTLVGNAANNLLDD506 pKa = 4.23GGAGADD512 pKa = 3.8VMNGGAGNDD521 pKa = 3.69TYY523 pKa = 11.69VVDD526 pKa = 6.13DD527 pKa = 4.92IGDD530 pKa = 3.77QIIDD534 pKa = 3.52SAGIDD539 pKa = 3.43TVLASISYY547 pKa = 8.81TLGNDD552 pKa = 3.85LEE554 pKa = 4.29NLSLLGSEE562 pKa = 4.71ALNGTGNGLANVITGNDD579 pKa = 3.61GSNVLDD585 pKa = 3.98GGGGVDD591 pKa = 3.54TLRR594 pKa = 11.84GGKK597 pKa = 10.24GDD599 pKa = 3.51DD600 pKa = 3.61TYY602 pKa = 11.35IVDD605 pKa = 4.17LTARR609 pKa = 11.84NTLEE613 pKa = 5.05DD614 pKa = 3.66SVLEE618 pKa = 3.95NLYY621 pKa = 10.4EE622 pKa = 4.51GNDD625 pKa = 3.96TIQLRR630 pKa = 11.84GGNVALATVTAIALANNVEE649 pKa = 4.21NLDD652 pKa = 3.7ASATGTTRR660 pKa = 11.84LNLTGNVLNNILIGNAADD678 pKa = 3.54NLLNGGAGADD688 pKa = 3.74RR689 pKa = 11.84LIGGDD694 pKa = 3.67GNDD697 pKa = 3.49TYY699 pKa = 11.8VVDD702 pKa = 3.8NLGDD706 pKa = 3.79EE707 pKa = 4.88VIEE710 pKa = 4.16QAGEE714 pKa = 4.16GTDD717 pKa = 3.66LVQVAIATAGGTYY730 pKa = 9.79TLGEE734 pKa = 4.22HH735 pKa = 6.25VEE737 pKa = 4.25NATLTNKK744 pKa = 9.53VAFNLTGNALSNTLVGNAANNVLDD768 pKa = 4.47GGLGADD774 pKa = 4.09VMNGGAGNDD783 pKa = 3.66TYY785 pKa = 11.14IVDD788 pKa = 4.4DD789 pKa = 4.34VGDD792 pKa = 4.15TIIDD796 pKa = 3.55SAGIDD801 pKa = 3.42TVRR804 pKa = 11.84ASIAYY809 pKa = 7.77TLGKK813 pKa = 10.42GLEE816 pKa = 4.1NLVLTGSHH824 pKa = 6.01AVSGTGNALANILDD838 pKa = 4.3GSQNNAANEE847 pKa = 3.72LRR849 pKa = 11.84GLGGNDD855 pKa = 3.39TYY857 pKa = 11.18IVGAGDD863 pKa = 3.74TVIEE867 pKa = 4.24EE868 pKa = 4.4ANGGIDD874 pKa = 4.05LVQAYY879 pKa = 9.61VSYY882 pKa = 9.93TLGDD886 pKa = 3.59HH887 pKa = 6.76VEE889 pKa = 4.06NLSLLGSEE897 pKa = 4.71ALNGTGNGLANVLTGNDD914 pKa = 3.65GSNVLDD920 pKa = 3.98GGGGVDD926 pKa = 3.54TLRR929 pKa = 11.84GGKK932 pKa = 10.24GDD934 pKa = 3.51DD935 pKa = 3.61TYY937 pKa = 11.37IVDD940 pKa = 4.97LMANNALEE948 pKa = 4.2DD949 pKa = 3.56RR950 pKa = 11.84VVEE953 pKa = 4.11NANEE957 pKa = 4.68GIDD960 pKa = 3.86TIVLRR965 pKa = 11.84GGSALPKK972 pKa = 10.15AATIVLANNVEE983 pKa = 4.07NLDD986 pKa = 3.64ASGTGSIALNLTGNAANNLLIGNQGNNTLNGGDD1019 pKa = 4.38GNDD1022 pKa = 3.64TLIGMGGFDD1031 pKa = 3.91TLTGGRR1037 pKa = 11.84GADD1040 pKa = 3.26TFRR1043 pKa = 11.84FALTWLGMNSGPDD1056 pKa = 3.32AVITDD1061 pKa = 3.82FRR1063 pKa = 11.84RR1064 pKa = 11.84LDD1066 pKa = 3.37GDD1068 pKa = 4.15KK1069 pKa = 10.68IQLQGEE1075 pKa = 4.1DD1076 pKa = 3.33AEE1078 pKa = 4.43RR1079 pKa = 11.84FSFVGRR1085 pKa = 11.84TFDD1088 pKa = 3.11TGATGSHH1095 pKa = 5.55QLRR1098 pKa = 11.84FDD1100 pKa = 3.54AGTLYY1105 pKa = 11.11GSVDD1109 pKa = 3.36SGMSNAFEE1117 pKa = 3.85IRR1119 pKa = 11.84LLGVSEE1125 pKa = 4.57LRR1127 pKa = 11.84QDD1129 pKa = 3.95DD1130 pKa = 4.16FLFGG1134 pKa = 3.75

Molecular weight: 117.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4XNA6|A4XNA6_PSEMY Type III secretion protein SpaR/YscT/HrcT OS=Pseudomonas mendocina (strain ymp) OX=399739 GN=Pmen_0048 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4563

0

4563

1497472

23

5328

328.2

36.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.557 ± 0.05

1.007 ± 0.011

5.281 ± 0.03

6.101 ± 0.03

3.528 ± 0.021

8.033 ± 0.034

2.258 ± 0.019

4.507 ± 0.026

3.094 ± 0.028

12.493 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.211 ± 0.018

2.742 ± 0.024

4.894 ± 0.025

4.798 ± 0.032

6.955 ± 0.039

5.518 ± 0.027

4.285 ± 0.025

6.796 ± 0.03

1.455 ± 0.016

2.488 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski