Bdellovibrio phage phi1422

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9C0P1|H9C0P1_9CAUD Putative tail-fiber protein OS=Bdellovibrio phage phi1422 OX=1127515 GN=phi1422_0067 PE=4 SV=1
MM1 pKa = 7.82TDD3 pKa = 3.37QEE5 pKa = 4.41LLQYY9 pKa = 10.72YY10 pKa = 10.68ADD12 pKa = 4.8LLILQYY18 pKa = 10.35RR19 pKa = 11.84DD20 pKa = 3.34KK21 pKa = 10.15PKK23 pKa = 10.95ASATVKK29 pKa = 10.61AYY31 pKa = 11.17VNLLISGQLPLAVQNAFDD49 pKa = 4.05IEE51 pKa = 4.48TAVGKK56 pKa = 10.1QLDD59 pKa = 4.39VIGKK63 pKa = 8.68IVGVTRR69 pKa = 11.84DD70 pKa = 3.43GYY72 pKa = 8.72TFSEE76 pKa = 4.66AIVLNDD82 pKa = 3.37DD83 pKa = 5.24DD84 pKa = 4.24YY85 pKa = 12.17RR86 pKa = 11.84KK87 pKa = 9.08MIKK90 pKa = 9.33MKK92 pKa = 10.48IFLNNSDD99 pKa = 3.47SSLYY103 pKa = 10.67SIQNLIALYY112 pKa = 9.68FQGLMKK118 pKa = 10.65VYY120 pKa = 10.05DD121 pKa = 4.19YY122 pKa = 11.69QDD124 pKa = 2.95MSMSYY129 pKa = 10.64ALSSAFGSQDD139 pKa = 3.12LVEE142 pKa = 4.22VFINKK147 pKa = 9.36GLLPRR152 pKa = 11.84PMGVGLGATIYY163 pKa = 10.32HH164 pKa = 6.46PTMKK168 pKa = 10.35FFGFRR173 pKa = 11.84TYY175 pKa = 9.84QAEE178 pKa = 4.25NPNAFPFNTYY188 pKa = 11.19DD189 pKa = 3.81NYY191 pKa = 11.44NEE193 pKa = 3.81QAPWLLYY200 pKa = 10.51AYY202 pKa = 9.61AVNATTTVDD211 pKa = 3.62EE212 pKa = 4.95LLLTEE217 pKa = 5.66DD218 pKa = 4.0GVNNIITEE226 pKa = 4.41DD227 pKa = 3.97GEE229 pKa = 4.88DD230 pKa = 3.24ILVV233 pKa = 3.49

Molecular weight: 26.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9C0K9|H9C0K9_9CAUD Uncharacterized protein OS=Bdellovibrio phage phi1422 OX=1127515 GN=phi1422_0035 PE=4 SV=1
MM1 pKa = 7.51IAHH4 pKa = 6.74DD5 pKa = 3.67VCYY8 pKa = 10.21RR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 10.42GKK13 pKa = 10.06IYY15 pKa = 10.25LMNARR20 pKa = 11.84RR21 pKa = 11.84QGEE24 pKa = 4.23YY25 pKa = 10.36FYY27 pKa = 10.55FGCKK31 pKa = 9.59LAVLTDD37 pKa = 3.51FTMKK41 pKa = 10.31KK42 pKa = 9.15EE43 pKa = 3.98KK44 pKa = 10.33SKK46 pKa = 11.58SEE48 pKa = 3.84IEE50 pKa = 4.05FKK52 pKa = 10.42KK53 pKa = 10.13QYY55 pKa = 10.58RR56 pKa = 11.84FARR59 pKa = 11.84LYY61 pKa = 9.31LQNRR65 pKa = 11.84VRR67 pKa = 11.84RR68 pKa = 11.84KK69 pKa = 9.35PDD71 pKa = 3.29DD72 pKa = 3.57YY73 pKa = 11.51YY74 pKa = 11.51AIYY77 pKa = 10.81SLAQRR82 pKa = 11.84EE83 pKa = 4.18IHH85 pKa = 6.52KK86 pKa = 11.26GNDD89 pKa = 2.83FRR91 pKa = 11.84SNKK94 pKa = 9.38LHH96 pKa = 6.31YY97 pKa = 10.17LAAKK101 pKa = 9.46QRR103 pKa = 11.84IRR105 pKa = 11.84NNQKK109 pKa = 9.94RR110 pKa = 11.84KK111 pKa = 9.94RR112 pKa = 11.84KK113 pKa = 9.4

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

14384

41

838

189.3

21.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.654 ± 0.367

1.224 ± 0.133

5.902 ± 0.203

7.175 ± 0.397

4.463 ± 0.221

6.73 ± 0.282

1.481 ± 0.169

6.521 ± 0.272

8.53 ± 0.446

7.613 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.704 ± 0.175

5.159 ± 0.249

3.692 ± 0.184

3.539 ± 0.208

4.394 ± 0.276

6.215 ± 0.259

5.659 ± 0.377

6.917 ± 0.255

1.196 ± 0.104

3.233 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski