Amino acid dipepetide frequency for Mycoplasma canis UFG4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.739AlaAla: 2.739 ± 0.214
0.221AlaCys: 0.221 ± 0.032
2.169AlaAsp: 2.169 ± 0.133
2.865AlaGlu: 2.865 ± 0.205
2.408AlaPhe: 2.408 ± 0.119
2.378AlaGly: 2.378 ± 0.143
0.727AlaHis: 0.727 ± 0.058
4.63AlaIle: 4.63 ± 0.168
5.372AlaLys: 5.372 ± 0.285
4.946AlaLeu: 4.946 ± 0.215
0.791AlaMet: 0.791 ± 0.057
3.519AlaAsn: 3.519 ± 0.171
1.088AlaPro: 1.088 ± 0.07
1.602AlaGln: 1.602 ± 0.109
1.838AlaArg: 1.838 ± 0.1
3.565AlaSer: 3.565 ± 0.107
2.743AlaThr: 2.743 ± 0.15
2.42AlaVal: 2.42 ± 0.145
0.339AlaTrp: 0.339 ± 0.032
1.678AlaTyr: 1.678 ± 0.094
0.0AlaXaa: 0.0 ± 0.0
Cys
0.186CysAla: 0.186 ± 0.032
0.019CysCys: 0.019 ± 0.007
0.19CysAsp: 0.19 ± 0.031
0.179CysGlu: 0.179 ± 0.028
0.16CysPhe: 0.16 ± 0.027
0.24CysGly: 0.24 ± 0.037
0.065CysHis: 0.065 ± 0.016
0.27CysIle: 0.27 ± 0.033
0.213CysLys: 0.213 ± 0.03
0.24CysLeu: 0.24 ± 0.034
0.03CysMet: 0.03 ± 0.01
0.183CysAsn: 0.183 ± 0.025
0.072CysPro: 0.072 ± 0.017
0.088CysGln: 0.088 ± 0.018
0.103CysArg: 0.103 ± 0.02
0.293CysSer: 0.293 ± 0.036
0.148CysThr: 0.148 ± 0.024
0.156CysVal: 0.156 ± 0.025
0.027CysTrp: 0.027 ± 0.011
0.08CysTyr: 0.08 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
2.72AspAla: 2.72 ± 0.174
0.122AspCys: 0.122 ± 0.024
2.758AspAsp: 2.758 ± 0.136
4.588AspGlu: 4.588 ± 0.132
3.751AspPhe: 3.751 ± 0.155
2.515AspGly: 2.515 ± 0.12
0.738AspHis: 0.738 ± 0.063
4.775AspIle: 4.775 ± 0.15
5.825AspLys: 5.825 ± 0.162
5.806AspLeu: 5.806 ± 0.167
0.635AspMet: 0.635 ± 0.049
4.242AspAsn: 4.242 ± 0.213
1.568AspPro: 1.568 ± 0.072
1.819AspGln: 1.819 ± 0.109
1.655AspArg: 1.655 ± 0.092
4.063AspSer: 4.063 ± 0.167
2.23AspThr: 2.23 ± 0.109
3.143AspVal: 3.143 ± 0.127
0.377AspTrp: 0.377 ± 0.037
2.652AspTyr: 2.652 ± 0.126
0.0AspXaa: 0.0 ± 0.0
Glu
3.664GluAla: 3.664 ± 0.265
0.114GluCys: 0.114 ± 0.022
3.333GluAsp: 3.333 ± 0.11
5.718GluGlu: 5.718 ± 0.251
4.368GluPhe: 4.368 ± 0.141
3.01GluGly: 3.01 ± 0.137
0.856GluHis: 0.856 ± 0.057
8.233GluIle: 8.233 ± 0.223
8.058GluLys: 8.058 ± 0.275
7.465GluLeu: 7.465 ± 0.236
1.316GluMet: 1.316 ± 0.083
6.883GluAsn: 6.883 ± 0.226
1.453GluPro: 1.453 ± 0.09
2.013GluGln: 2.013 ± 0.104
2.309GluArg: 2.309 ± 0.107
4.257GluSer: 4.257 ± 0.125
3.782GluThr: 3.782 ± 0.144
4.315GluVal: 4.315 ± 0.154
0.613GluTrp: 0.613 ± 0.051
3.188GluTyr: 3.188 ± 0.132
0.0GluXaa: 0.0 ± 0.0
Phe
2.534PheAla: 2.534 ± 0.113
0.183PheCys: 0.183 ± 0.027
3.843PheAsp: 3.843 ± 0.128
4.269PheGlu: 4.269 ± 0.155
2.758PhePhe: 2.758 ± 0.158
2.751PheGly: 2.751 ± 0.145
0.613PheHis: 0.613 ± 0.057
5.418PheIle: 5.418 ± 0.225
5.604PheLys: 5.604 ± 0.168
4.946PheLeu: 4.946 ± 0.222
0.925PheMet: 0.925 ± 0.07
4.269PheAsn: 4.269 ± 0.186
1.191PhePro: 1.191 ± 0.081
1.366PheGln: 1.366 ± 0.084
1.541PheArg: 1.541 ± 0.094
4.474PheSer: 4.474 ± 0.181
2.515PheThr: 2.515 ± 0.126
3.398PheVal: 3.398 ± 0.143
0.616PheTrp: 0.616 ± 0.051
2.279PheTyr: 2.279 ± 0.155
0.0PheXaa: 0.0 ± 0.0
Gly
2.564GlyAla: 2.564 ± 0.131
0.179GlyCys: 0.179 ± 0.026
2.424GlyAsp: 2.424 ± 0.108
2.823GlyGlu: 2.823 ± 0.133
2.854GlyPhe: 2.854 ± 0.128
2.838GlyGly: 2.838 ± 0.169
0.769GlyHis: 0.769 ± 0.066
4.52GlyIle: 4.52 ± 0.199
4.025GlyLys: 4.025 ± 0.177
3.93GlyLeu: 3.93 ± 0.152
0.928GlyMet: 0.928 ± 0.069
2.812GlyAsn: 2.812 ± 0.145
0.86GlyPro: 0.86 ± 0.073
1.396GlyGln: 1.396 ± 0.083
1.644GlyArg: 1.644 ± 0.095
3.363GlySer: 3.363 ± 0.133
2.64GlyThr: 2.64 ± 0.126
2.812GlyVal: 2.812 ± 0.139
0.441GlyTrp: 0.441 ± 0.045
2.024GlyTyr: 2.024 ± 0.109
0.0GlyXaa: 0.0 ± 0.0
His
0.647HisAla: 0.647 ± 0.054
0.061HisCys: 0.061 ± 0.017
0.689HisAsp: 0.689 ± 0.052
0.97HisGlu: 0.97 ± 0.063
0.909HisPhe: 0.909 ± 0.067
0.803HisGly: 0.803 ± 0.065
0.224HisHis: 0.224 ± 0.034
1.225HisIle: 1.225 ± 0.078
1.263HisLys: 1.263 ± 0.077
1.202HisLeu: 1.202 ± 0.068
0.186HisMet: 0.186 ± 0.027
1.115HisAsn: 1.115 ± 0.067
0.491HisPro: 0.491 ± 0.051
0.361HisGln: 0.361 ± 0.042
0.35HisArg: 0.35 ± 0.042
0.963HisSer: 0.963 ± 0.056
0.613HisThr: 0.613 ± 0.05
0.666HisVal: 0.666 ± 0.052
0.084HisTrp: 0.084 ± 0.019
0.472HisTyr: 0.472 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
4.783IleAla: 4.783 ± 0.167
0.339IleCys: 0.339 ± 0.041
5.928IleAsp: 5.928 ± 0.199
6.761IleGlu: 6.761 ± 0.192
5.384IlePhe: 5.384 ± 0.222
3.843IleGly: 3.843 ± 0.173
1.221IleHis: 1.221 ± 0.07
8.306IleIle: 8.306 ± 0.274
9.98IleLys: 9.98 ± 0.252
8.26IleLeu: 8.26 ± 0.283
1.233IleMet: 1.233 ± 0.087
8.903IleAsn: 8.903 ± 0.394
2.564IlePro: 2.564 ± 0.114
2.633IleGln: 2.633 ± 0.114
2.424IleArg: 2.424 ± 0.128
8.066IleSer: 8.066 ± 0.287
4.634IleThr: 4.634 ± 0.155
4.946IleVal: 4.946 ± 0.182
0.692IleTrp: 0.692 ± 0.05
3.497IleTyr: 3.497 ± 0.147
0.0IleXaa: 0.0 ± 0.0
Lys
4.455LysAla: 4.455 ± 0.239
0.179LysCys: 0.179 ± 0.028
5.867LysAsp: 5.867 ± 0.181
9.39LysGlu: 9.39 ± 0.31
4.954LysPhe: 4.954 ± 0.183
3.626LysGly: 3.626 ± 0.169
1.335LysHis: 1.335 ± 0.074
10.208LysIle: 10.208 ± 0.288
10.531LysLys: 10.531 ± 0.286
8.949LysLeu: 8.949 ± 0.192
2.287LysMet: 2.287 ± 0.113
10.041LysAsn: 10.041 ± 0.274
2.606LysPro: 2.606 ± 0.153
2.998LysGln: 2.998 ± 0.108
3.181LysArg: 3.181 ± 0.14
6.08LysSer: 6.08 ± 0.154
5.395LysThr: 5.395 ± 0.151
5.745LysVal: 5.745 ± 0.167
0.963LysTrp: 0.963 ± 0.066
4.471LysTyr: 4.471 ± 0.187
0.0LysXaa: 0.0 ± 0.0
Leu
4.467LeuAla: 4.467 ± 0.19
0.263LeuCys: 0.263 ± 0.036
5.319LeuAsp: 5.319 ± 0.171
6.936LeuGlu: 6.936 ± 0.186
5.034LeuPhe: 5.034 ± 0.21
4.307LeuGly: 4.307 ± 0.206
1.092LeuHis: 1.092 ± 0.065
8.123LeuIle: 8.123 ± 0.227
9.74LeuLys: 9.74 ± 0.232
7.727LeuLeu: 7.727 ± 0.222
1.457LeuMet: 1.457 ± 0.09
8.256LeuAsn: 8.256 ± 0.337
2.743LeuPro: 2.743 ± 0.119
2.203LeuGln: 2.203 ± 0.092
2.937LeuArg: 2.937 ± 0.126
7.895LeuSer: 7.895 ± 0.2
4.695LeuThr: 4.695 ± 0.144
5.11LeuVal: 5.11 ± 0.14
0.723LeuTrp: 0.723 ± 0.057
3.006LeuTyr: 3.006 ± 0.117
0.0LeuXaa: 0.0 ± 0.0
Met
0.731MetAla: 0.731 ± 0.071
0.08MetCys: 0.08 ± 0.019
0.761MetAsp: 0.761 ± 0.063
0.856MetGlu: 0.856 ± 0.06
0.871MetPhe: 0.871 ± 0.072
0.662MetGly: 0.662 ± 0.067
0.259MetHis: 0.259 ± 0.029
1.56MetIle: 1.56 ± 0.094
1.849MetLys: 1.849 ± 0.102
1.503MetLeu: 1.503 ± 0.093
0.35MetMet: 0.35 ± 0.043
1.259MetAsn: 1.259 ± 0.071
0.582MetPro: 0.582 ± 0.049
0.506MetGln: 0.506 ± 0.044
0.514MetArg: 0.514 ± 0.042
1.21MetSer: 1.21 ± 0.075
0.833MetThr: 0.833 ± 0.06
0.814MetVal: 0.814 ± 0.063
0.129MetTrp: 0.129 ± 0.02
0.449MetTyr: 0.449 ± 0.05
0.0MetXaa: 0.0 ± 0.0
Asn
3.47AsnAla: 3.47 ± 0.227
0.148AsnCys: 0.148 ± 0.027
5.072AsnAsp: 5.072 ± 0.186
7.275AsnGlu: 7.275 ± 0.29
4.197AsnPhe: 4.197 ± 0.197
3.873AsnGly: 3.873 ± 0.182
1.081AsnHis: 1.081 ± 0.07
8.302AsnIle: 8.302 ± 0.315
9.382AsnLys: 9.382 ± 0.268
7.735AsnLeu: 7.735 ± 0.293
0.913AsnMet: 0.913 ± 0.074
8.732AsnAsn: 8.732 ± 0.613
2.644AsnPro: 2.644 ± 0.121
2.557AsnGln: 2.557 ± 0.142
2.112AsnArg: 2.112 ± 0.124
6.78AsnSer: 6.78 ± 0.344
3.687AsnThr: 3.687 ± 0.18
4.086AsnVal: 4.086 ± 0.194
0.818AsnTrp: 0.818 ± 0.068
3.333AsnTyr: 3.333 ± 0.131
0.0AsnXaa: 0.0 ± 0.0
Pro
1.29ProAla: 1.29 ± 0.101
0.061ProCys: 0.061 ± 0.016
1.396ProAsp: 1.396 ± 0.082
2.283ProGlu: 2.283 ± 0.129
1.351ProPhe: 1.351 ± 0.086
1.51ProGly: 1.51 ± 0.08
0.479ProHis: 0.479 ± 0.048
2.172ProIle: 2.172 ± 0.098
2.287ProLys: 2.287 ± 0.132
1.997ProLeu: 1.997 ± 0.091
0.43ProMet: 0.43 ± 0.04
2.066ProAsn: 2.066 ± 0.11
0.365ProPro: 0.365 ± 0.05
0.78ProGln: 0.78 ± 0.059
0.711ProArg: 0.711 ± 0.06
2.108ProSer: 2.108 ± 0.122
1.556ProThr: 1.556 ± 0.082
1.362ProVal: 1.362 ± 0.099
0.247ProTrp: 0.247 ± 0.033
1.103ProTyr: 1.103 ± 0.072
0.0ProXaa: 0.0 ± 0.0
Gln
1.495GlnAla: 1.495 ± 0.132
0.049GlnCys: 0.049 ± 0.013
1.465GlnAsp: 1.465 ± 0.073
2.367GlnGlu: 2.367 ± 0.103
1.282GlnPhe: 1.282 ± 0.069
1.191GlnGly: 1.191 ± 0.066
0.346GlnHis: 0.346 ± 0.034
2.865GlnIle: 2.865 ± 0.112
3.188GlnLys: 3.188 ± 0.117
2.386GlnLeu: 2.386 ± 0.103
0.502GlnMet: 0.502 ± 0.053
2.8GlnAsn: 2.8 ± 0.128
0.807GlnPro: 0.807 ± 0.102
0.822GlnGln: 0.822 ± 0.056
1.008GlnArg: 1.008 ± 0.071
1.708GlnSer: 1.708 ± 0.076
1.442GlnThr: 1.442 ± 0.073
1.568GlnVal: 1.568 ± 0.087
0.186GlnTrp: 0.186 ± 0.035
0.951GlnTyr: 0.951 ± 0.064
0.0GlnXaa: 0.0 ± 0.0
Arg
1.564ArgAla: 1.564 ± 0.118
0.068ArgCys: 0.068 ± 0.019
1.659ArgAsp: 1.659 ± 0.098
2.172ArgGlu: 2.172 ± 0.124
1.514ArgPhe: 1.514 ± 0.063
1.362ArgGly: 1.362 ± 0.093
0.396ArgHis: 0.396 ± 0.045
3.089ArgIle: 3.089 ± 0.146
3.055ArgLys: 3.055 ± 0.124
2.751ArgLeu: 2.751 ± 0.137
0.673ArgMet: 0.673 ± 0.059
2.488ArgAsn: 2.488 ± 0.121
0.829ArgPro: 0.829 ± 0.06
0.856ArgGln: 0.856 ± 0.055
1.157ArgArg: 1.157 ± 0.084
1.902ArgSer: 1.902 ± 0.088
1.621ArgThr: 1.621 ± 0.089
1.727ArgVal: 1.727 ± 0.091
0.263ArgTrp: 0.263 ± 0.031
1.24ArgTyr: 1.24 ± 0.077
0.0ArgXaa: 0.0 ± 0.0
Ser
3.154SerAla: 3.154 ± 0.11
0.377SerCys: 0.377 ± 0.039
4.181SerAsp: 4.181 ± 0.147
5.3SerGlu: 5.3 ± 0.153
4.55SerPhe: 4.55 ± 0.173
3.812SerGly: 3.812 ± 0.155
0.97SerHis: 0.97 ± 0.061
6.898SerIle: 6.898 ± 0.217
7.575SerLys: 7.575 ± 0.179
7.625SerLeu: 7.625 ± 0.23
1.02SerMet: 1.02 ± 0.072
6.419SerAsn: 6.419 ± 0.393
1.374SerPro: 1.374 ± 0.078
2.328SerGln: 2.328 ± 0.095
2.26SerArg: 2.26 ± 0.098
6.228SerSer: 6.228 ± 0.195
3.371SerThr: 3.371 ± 0.108
3.706SerVal: 3.706 ± 0.133
0.666SerTrp: 0.666 ± 0.047
3.082SerTyr: 3.082 ± 0.12
0.0SerXaa: 0.0 ± 0.0
Thr
2.207ThrAla: 2.207 ± 0.118
0.114ThrCys: 0.114 ± 0.024
2.389ThrAsp: 2.389 ± 0.111
3.063ThrGlu: 3.063 ± 0.138
3.166ThrPhe: 3.166 ± 0.163
2.401ThrGly: 2.401 ± 0.115
0.829ThrHis: 0.829 ± 0.054
4.596ThrIle: 4.596 ± 0.174
5.026ThrLys: 5.026 ± 0.14
4.904ThrLeu: 4.904 ± 0.145
0.601ThrMet: 0.601 ± 0.05
4.349ThrAsn: 4.349 ± 0.214
1.541ThrPro: 1.541 ± 0.086
1.507ThrGln: 1.507 ± 0.079
1.609ThrArg: 1.609 ± 0.082
3.984ThrSer: 3.984 ± 0.148
2.88ThrThr: 2.88 ± 0.143
2.637ThrVal: 2.637 ± 0.131
0.415ThrTrp: 0.415 ± 0.042
1.8ThrTyr: 1.8 ± 0.083
0.0ThrXaa: 0.0 ± 0.0
Val
3.105ValAla: 3.105 ± 0.137
0.224ValCys: 0.224 ± 0.028
3.645ValAsp: 3.645 ± 0.145
3.911ValGlu: 3.911 ± 0.122
3.063ValPhe: 3.063 ± 0.13
2.492ValGly: 2.492 ± 0.132
0.708ValHis: 0.708 ± 0.048
4.931ValIle: 4.931 ± 0.176
5.049ValLys: 5.049 ± 0.16
5.133ValLeu: 5.133 ± 0.152
0.791ValMet: 0.791 ± 0.061
4.105ValAsn: 4.105 ± 0.181
1.541ValPro: 1.541 ± 0.091
1.457ValGln: 1.457 ± 0.083
1.526ValArg: 1.526 ± 0.081
4.36ValSer: 4.36 ± 0.148
2.701ValThr: 2.701 ± 0.137
3.447ValVal: 3.447 ± 0.151
0.468ValTrp: 0.468 ± 0.038
2.032ValTyr: 2.032 ± 0.097
0.0ValXaa: 0.0 ± 0.0
Trp
0.449TrpAla: 0.449 ± 0.047
0.027TrpCys: 0.027 ± 0.013
0.476TrpAsp: 0.476 ± 0.04
0.548TrpGlu: 0.548 ± 0.051
0.567TrpPhe: 0.567 ± 0.05
0.388TrpGly: 0.388 ± 0.048
0.084TrpHis: 0.084 ± 0.018
0.921TrpIle: 0.921 ± 0.062
0.871TrpLys: 0.871 ± 0.066
0.692TrpLeu: 0.692 ± 0.055
0.156TrpMet: 0.156 ± 0.027
0.662TrpAsn: 0.662 ± 0.057
0.202TrpPro: 0.202 ± 0.025
0.183TrpGln: 0.183 ± 0.031
0.274TrpArg: 0.274 ± 0.033
0.62TrpSer: 0.62 ± 0.046
0.552TrpThr: 0.552 ± 0.049
0.517TrpVal: 0.517 ± 0.051
0.145TrpTrp: 0.145 ± 0.027
0.354TrpTyr: 0.354 ± 0.039
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.815TyrAla: 1.815 ± 0.089
0.114TyrCys: 0.114 ± 0.021
2.42TyrAsp: 2.42 ± 0.105
2.675TyrGlu: 2.675 ± 0.12
2.351TyrPhe: 2.351 ± 0.125
1.845TyrGly: 1.845 ± 0.087
0.502TyrHis: 0.502 ± 0.049
3.352TyrIle: 3.352 ± 0.14
4.368TyrLys: 4.368 ± 0.168
3.835TyrLeu: 3.835 ± 0.169
0.548TyrMet: 0.548 ± 0.041
3.093TyrAsn: 3.093 ± 0.124
1.035TyrPro: 1.035 ± 0.084
0.955TyrGln: 0.955 ± 0.065
1.237TyrArg: 1.237 ± 0.071
2.975TyrSer: 2.975 ± 0.112
1.978TyrThr: 1.978 ± 0.103
2.115TyrVal: 2.115 ± 0.104
0.476TyrTrp: 0.476 ± 0.044
1.446TyrTyr: 1.446 ± 0.088
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 642 proteins (262833 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski