Conexibacter woesei (strain DSM 14684 / CIP 108061 / JCM 11494 / NBRC 100937 / ID131577)
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5912 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D3F094|D3F094_CONWI Major facilitator superfamily MFS_1 OS=Conexibacter woesei (strain DSM 14684 / CIP 108061 / JCM 11494 / NBRC 100937 / ID131577) OX=469383 GN=Cwoe_3536 PE=4 SV=1
MM1 pKa = 7.57 SATQTSQKK9 pKa = 9.16 WICEE13 pKa = 4.24 SCGFIYY19 pKa = 10.56 DD20 pKa = 4.59 PEE22 pKa = 6.8 DD23 pKa = 4.11 GDD25 pKa = 4.31 PDD27 pKa = 3.95 GGIPPGTAFEE37 pKa = 5.84 DD38 pKa = 3.83 IPEE41 pKa = 4.12 TWFCPVCGARR51 pKa = 11.84 KK52 pKa = 9.28 RR53 pKa = 11.84 DD54 pKa = 3.61 FVPYY58 pKa = 10.36 EE59 pKa = 3.91 DD60 pKa = 3.89
Molecular weight: 6.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.884
IPC_protein 3.783
Toseland 3.592
ProMoST 3.872
Dawson 3.783
Bjellqvist 4.024
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.503
Solomon 3.757
Lehninger 3.719
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.91
Patrickios 0.693
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.886
Protein with the highest isoelectric point:
>tr|D3F9J2|D3F9J2_CONWI Usp domain-containing protein OS=Conexibacter woesei (strain DSM 14684 / CIP 108061 / JCM 11494 / NBRC 100937 / ID131577) OX=469383 GN=Cwoe_0726 PE=4 SV=1
MM1 pKa = 7.56 RR2 pKa = 11.84 ASIAAKK8 pKa = 10.07 KK9 pKa = 8.81 RR10 pKa = 11.84 TASAMAIKK18 pKa = 10.44 SMPSLWGRR26 pKa = 11.84 PRR28 pKa = 11.84 PGRR31 pKa = 11.84 IKK33 pKa = 10.45 IQTAGIKK40 pKa = 9.28 MLL42 pKa = 4.23
Molecular weight: 4.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.408
IPC2_protein 10.891
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.457
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.193
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.023
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5912
0
5912
1985113
31
4255
335.8
35.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.9 ± 0.06
0.779 ± 0.008
6.001 ± 0.026
5.739 ± 0.038
2.829 ± 0.016
9.582 ± 0.039
1.966 ± 0.015
3.644 ± 0.023
1.462 ± 0.018
10.486 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.617 ± 0.012
1.676 ± 0.019
5.776 ± 0.03
2.651 ± 0.016
8.727 ± 0.033
4.855 ± 0.026
5.587 ± 0.036
8.594 ± 0.027
1.32 ± 0.014
1.811 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here