Apis mellifera associated microvirus 2

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTI6|A0A3S8UTI6_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 2 OX=2494747 PE=4 SV=1
MM1 pKa = 7.39AKK3 pKa = 10.16EE4 pKa = 4.44SVFLRR9 pKa = 11.84TPYY12 pKa = 10.64NYY14 pKa = 10.79DD15 pKa = 2.96RR16 pKa = 11.84MAVSDD21 pKa = 4.04EE22 pKa = 4.3TGLFCDD28 pKa = 5.37DD29 pKa = 4.4PSLTQQHH36 pKa = 6.69DD37 pKa = 3.6MADD40 pKa = 3.58CDD42 pKa = 3.48INNIVEE48 pKa = 4.06RR49 pKa = 11.84AARR52 pKa = 11.84TGFIPNAVGSPFVGDD67 pKa = 3.61LAINATDD74 pKa = 3.44FHH76 pKa = 6.54EE77 pKa = 4.41AMNIVVSARR86 pKa = 11.84QAFSEE91 pKa = 4.2LDD93 pKa = 3.09AKK95 pKa = 10.02TRR97 pKa = 11.84ARR99 pKa = 11.84FGNDD103 pKa = 2.88PGQLLEE109 pKa = 4.08FLQDD113 pKa = 3.05EE114 pKa = 4.69GNYY117 pKa = 10.81DD118 pKa = 3.73EE119 pKa = 5.32ASKK122 pKa = 11.03LGLVPPRR129 pKa = 11.84EE130 pKa = 3.96EE131 pKa = 3.87LSIGEE136 pKa = 4.0RR137 pKa = 11.84SANKK141 pKa = 9.58SSRR144 pKa = 11.84RR145 pKa = 11.84SSPVEE150 pKa = 3.6NSASSDD156 pKa = 3.45FDD158 pKa = 4.88SDD160 pKa = 4.08DD161 pKa = 3.57SQDD164 pKa = 3.83RR165 pKa = 11.84VAEE168 pKa = 4.13LQAQLQRR175 pKa = 11.84LQGSSSKK182 pKa = 10.62KK183 pKa = 9.41GVSSPFRR190 pKa = 11.84RR191 pKa = 11.84GSGDD195 pKa = 3.26EE196 pKa = 3.89

Molecular weight: 21.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTJ6|A0A3S8UTJ6_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 2 OX=2494747 PE=3 SV=1
MM1 pKa = 7.08VCYY4 pKa = 9.87FPITGYY10 pKa = 10.3QSQPGAPLVFGGIAVNADD28 pKa = 3.2KK29 pKa = 10.99TPIKK33 pKa = 10.17VPCGRR38 pKa = 11.84CEE40 pKa = 4.19GCNEE44 pKa = 3.9RR45 pKa = 11.84YY46 pKa = 10.14ARR48 pKa = 11.84DD49 pKa = 2.92WAIRR53 pKa = 11.84MMHH56 pKa = 6.23EE57 pKa = 3.72ASLYY61 pKa = 9.82KK62 pKa = 10.33HH63 pKa = 6.17NCFITLTYY71 pKa = 10.87NNEE74 pKa = 3.93NLPPDD79 pKa = 3.59RR80 pKa = 11.84SLHH83 pKa = 5.35YY84 pKa = 10.49EE85 pKa = 3.99HH86 pKa = 6.72FQEE89 pKa = 4.15FMKK92 pKa = 10.52RR93 pKa = 11.84FRR95 pKa = 11.84EE96 pKa = 4.1AHH98 pKa = 5.53QGCDD102 pKa = 3.75LVSHH106 pKa = 7.22PYY108 pKa = 9.65FGKK111 pKa = 9.83IDD113 pKa = 3.63KK114 pKa = 8.96KK115 pKa = 9.21TGKK118 pKa = 9.25PYY120 pKa = 10.39PEE122 pKa = 4.82FYY124 pKa = 10.48RR125 pKa = 11.84PIRR128 pKa = 11.84HH129 pKa = 5.26YY130 pKa = 11.04VAGEE134 pKa = 3.77YY135 pKa = 10.25GGKK138 pKa = 9.88LGRR141 pKa = 11.84PHH143 pKa = 5.98WHH145 pKa = 5.94ACIFNFDD152 pKa = 3.78FADD155 pKa = 3.69KK156 pKa = 10.53YY157 pKa = 10.49IWEE160 pKa = 4.47TSTSGEE166 pKa = 3.92PLYY169 pKa = 10.52RR170 pKa = 11.84SPSLEE175 pKa = 3.84QLWPFGYY182 pKa = 10.38SSIGSVTFQSAAYY195 pKa = 8.01VARR198 pKa = 11.84YY199 pKa = 9.05INKK202 pKa = 9.28KK203 pKa = 9.91VKK205 pKa = 10.17GNQADD210 pKa = 3.83DD211 pKa = 3.73HH212 pKa = 6.28YY213 pKa = 11.06AWHH216 pKa = 7.13DD217 pKa = 3.7PEE219 pKa = 4.4TGEE222 pKa = 4.38VFWRR226 pKa = 11.84RR227 pKa = 11.84PEE229 pKa = 3.84FSKK232 pKa = 10.68MSLKK236 pKa = 10.58PGIGAGWLAKK246 pKa = 10.52YY247 pKa = 8.65KK248 pKa = 10.41TDD250 pKa = 4.13VFPHH254 pKa = 6.02DD255 pKa = 5.21HH256 pKa = 6.99IISDD260 pKa = 4.07GRR262 pKa = 11.84PLPVPRR268 pKa = 11.84YY269 pKa = 8.34YY270 pKa = 11.19SKK272 pKa = 10.48IYY274 pKa = 10.21QCTNPLEE281 pKa = 4.32WDD283 pKa = 3.44AVAHH287 pKa = 5.1EE288 pKa = 5.1RR289 pKa = 11.84YY290 pKa = 9.75VKK292 pKa = 10.4SRR294 pKa = 11.84QTLDD298 pKa = 3.61DD299 pKa = 3.78NTPEE303 pKa = 3.8RR304 pKa = 11.84LAVKK308 pKa = 10.13RR309 pKa = 11.84QVHH312 pKa = 5.43LAKK315 pKa = 10.67LSRR318 pKa = 11.84LKK320 pKa = 10.17RR321 pKa = 11.84TLKK324 pKa = 10.74

Molecular weight: 37.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1366

86

533

273.2

30.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.81 ± 1.68

1.025 ± 0.393

5.93 ± 1.155

4.173 ± 0.981

5.198 ± 0.422

7.101 ± 0.779

2.709 ± 0.581

4.026 ± 0.292

4.246 ± 1.058

7.394 ± 0.409

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.343 ± 0.477

4.832 ± 0.576

5.49 ± 0.681

4.612 ± 0.538

5.637 ± 0.703

7.687 ± 0.986

6.735 ± 1.291

5.564 ± 0.49

1.464 ± 0.376

4.026 ± 1.072
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski