Rehmannia virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Closterovirus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385HW67|A0A385HW67_9CLOS 20 kDa protein OS=Rehmannia virus 1 OX=2316740 GN=ORF7 PE=4 SV=1
MM1 pKa = 7.31TSHH4 pKa = 6.87NFGEE8 pKa = 4.52VNIVVPSTRR17 pKa = 11.84SGDD20 pKa = 4.05AIVLKK25 pKa = 9.3STPLCDD31 pKa = 3.62VVLLVDD37 pKa = 3.88ADD39 pKa = 4.28RR40 pKa = 11.84NEE42 pKa = 4.22DD43 pKa = 3.66CLVFLEE49 pKa = 5.62DD50 pKa = 3.4SLLMKK55 pKa = 10.3RR56 pKa = 11.84GKK58 pKa = 10.54SSDD61 pKa = 3.41EE62 pKa = 4.02LTCGRR67 pKa = 11.84DD68 pKa = 3.07LTMVKK73 pKa = 9.72IEE75 pKa = 4.22DD76 pKa = 3.89CMAEE80 pKa = 3.95YY81 pKa = 10.46HH82 pKa = 6.31SANLRR87 pKa = 11.84NPRR90 pKa = 11.84LLPVANRR97 pKa = 11.84HH98 pKa = 5.42SNCLSYY104 pKa = 11.44VDD106 pKa = 6.08FILSPVSSRR115 pKa = 11.84AVFRR119 pKa = 11.84MNLSNILVDD128 pKa = 3.71DD129 pKa = 4.14TEE131 pKa = 4.88VYY133 pKa = 10.27QDD135 pKa = 4.25SLVKK139 pKa = 10.56LDD141 pKa = 4.51GYY143 pKa = 9.61WDD145 pKa = 3.59NGAQHH150 pKa = 6.49QSMFQYY156 pKa = 10.8LSNFINYY163 pKa = 7.23EE164 pKa = 3.97KK165 pKa = 11.15YY166 pKa = 10.77SIDD169 pKa = 3.45SFEE172 pKa = 4.5CFLVV176 pKa = 3.53

Molecular weight: 19.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385HW53|A0A385HW53_9CLOS 21 kDa protein OS=Rehmannia virus 1 OX=2316740 GN=ORF8 PE=4 SV=1
MM1 pKa = 8.05DD2 pKa = 3.76NTLRR6 pKa = 11.84AYY8 pKa = 10.69LYY10 pKa = 11.1LLLGWVIICFCLTVSYY26 pKa = 10.81LVFKK30 pKa = 7.89FTRR33 pKa = 11.84SCTSVYY39 pKa = 10.83GDD41 pKa = 3.07IVEE44 pKa = 4.34TSVVGSSRR52 pKa = 11.84RR53 pKa = 11.84FEE55 pKa = 4.18SANSVEE61 pKa = 4.42VSRR64 pKa = 11.84AA65 pKa = 3.14

Molecular weight: 7.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

7364

65

2925

818.2

92.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.934 ± 0.337

2.567 ± 0.119

6.165 ± 0.256

5.5 ± 0.146

5.921 ± 0.406

5.296 ± 0.251

2.173 ± 0.207

5.486 ± 0.159

5.554 ± 0.314

9.845 ± 0.362

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.108

3.829 ± 0.603

3.504 ± 0.232

1.874 ± 0.14

6.749 ± 0.353

10.076 ± 0.406

4.699 ± 0.288

8.474 ± 0.521

0.543 ± 0.093

3.707 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski