Streptococcus satellite phage Javan751
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A0W9|A0A4D6A0W9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan751 OX=2558846 GN=JavanS751_0021 PE=4 SV=1
MM1 pKa = 7.22 TLDD4 pKa = 4.01 LGKK7 pKa = 8.39 MTQAEE12 pKa = 4.38 FDD14 pKa = 3.47 EE15 pKa = 4.84 AMADD19 pKa = 3.1 IKK21 pKa = 11.13 ARR23 pKa = 11.84 NPNLLQFITDD33 pKa = 4.19 FLDD36 pKa = 4.24 GKK38 pKa = 8.05 VTPEE42 pKa = 4.14 EE43 pKa = 4.01 VDD45 pKa = 3.54 DD46 pKa = 4.24
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.433
IPC2_protein 3.923
IPC_protein 3.821
Toseland 3.617
ProMoST 3.897
Dawson 3.821
Bjellqvist 4.075
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.948
Patrickios 4.05
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.908
Protein with the highest isoelectric point:
>tr|A0A4D5ZW90|A0A4D5ZW90_9VIRU Putative transcriptional regulator OS=Streptococcus satellite phage Javan751 OX=2558846 GN=JavanS751_0003 PE=4 SV=1
MM1 pKa = 7.42 SKK3 pKa = 10.54 KK4 pKa = 9.9 KK5 pKa = 10.34 ACTVRR10 pKa = 11.84 ACFAFPKK17 pKa = 9.79 WNSKK21 pKa = 8.86 KK22 pKa = 10.64 LKK24 pKa = 10.54 NEE26 pKa = 3.87 LKK28 pKa = 9.05 HH29 pKa = 5.48 TKK31 pKa = 9.84 KK32 pKa = 10.52 GSKK35 pKa = 9.27 EE36 pKa = 3.39 RR37 pKa = 11.84 WIVEE41 pKa = 3.92 NISYY45 pKa = 10.66 INALGIQVLDD55 pKa = 4.03 LLILRR60 pKa = 11.84 AITHH64 pKa = 6.47 FF65 pKa = 3.89
Molecular weight: 7.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.107
IPC2_protein 9.428
IPC_protein 9.355
Toseland 10.526
ProMoST 9.955
Dawson 10.599
Bjellqvist 10.16
Wikipedia 10.672
Rodwell 11.374
Grimsley 10.613
Solomon 10.628
Lehninger 10.613
Nozaki 10.511
DTASelect 10.145
Thurlkill 10.496
EMBOSS 10.891
Sillero 10.511
Patrickios 11.14
IPC_peptide 10.643
IPC2_peptide 8.712
IPC2.peptide.svr19 8.387
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21
0
21
2819
46
387
134.2
15.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.137 ± 0.408
0.709 ± 0.145
5.605 ± 0.362
8.868 ± 0.807
3.583 ± 0.318
4.47 ± 0.306
1.49 ± 0.215
6.846 ± 0.509
10.642 ± 0.39
10.5 ± 0.526
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.051 ± 0.353
5.534 ± 0.493
2.341 ± 0.179
4.718 ± 0.229
4.115 ± 0.36
5.25 ± 0.376
6.031 ± 0.328
4.789 ± 0.488
1.135 ± 0.148
4.186 ± 0.367
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here