Pectobacterium phage ZF40

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9C152|H9C152_9CAUD Putative integrase OS=Pectobacterium phage ZF40 OX=1127516 GN=ZF40_0002 PE=3 SV=1
MM1 pKa = 8.28DD2 pKa = 4.53ILGMEE7 pKa = 5.01TKK9 pKa = 10.17RR10 pKa = 11.84SAIIYY15 pKa = 8.39SQLIMEE21 pKa = 5.45IINYY25 pKa = 9.76NGTTICVLNNSDD37 pKa = 4.74MLTDD41 pKa = 4.56CPTGAYY47 pKa = 9.8AVIEE51 pKa = 4.33DD52 pKa = 3.86SGHH55 pKa = 5.08YY56 pKa = 9.03VAVRR60 pKa = 11.84VGEE63 pKa = 4.02TDD65 pKa = 3.0APAIHH70 pKa = 7.24IDD72 pKa = 3.74PVPTLEE78 pKa = 4.99DD79 pKa = 3.7ALDD82 pKa = 4.28IIAGRR87 pKa = 11.84LSFFAA92 pKa = 5.52

Molecular weight: 9.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9C187|H9C187_9CAUD Putative DNA polymerase OS=Pectobacterium phage ZF40 OX=1127516 GN=ZF40_0037 PE=4 SV=1
MM1 pKa = 7.36HH2 pKa = 7.57RR3 pKa = 11.84HH4 pKa = 5.38NKK6 pKa = 9.0PPVGHH11 pKa = 6.83KK12 pKa = 10.22FSIGLRR18 pKa = 11.84DD19 pKa = 3.22SSGNLVGVAMAGRR32 pKa = 11.84PVARR36 pKa = 11.84HH37 pKa = 5.81FDD39 pKa = 3.77DD40 pKa = 5.43GLTLEE45 pKa = 4.39VNRR48 pKa = 11.84TCTDD52 pKa = 2.95GTRR55 pKa = 11.84NANSMLYY62 pKa = 10.08GAVRR66 pKa = 11.84RR67 pKa = 11.84TAWEE71 pKa = 3.57MGYY74 pKa = 10.31CRR76 pKa = 11.84IITYY80 pKa = 10.01SQADD84 pKa = 3.57EE85 pKa = 4.54SGASLRR91 pKa = 11.84AAGFVLIKK99 pKa = 10.36NIPARR104 pKa = 11.84GSWSEE109 pKa = 3.99SSVKK113 pKa = 10.15RR114 pKa = 11.84RR115 pKa = 11.84TGRR118 pKa = 11.84DD119 pKa = 3.06PVGNGGVARR128 pKa = 11.84QLWEE132 pKa = 3.75VRR134 pKa = 11.84RR135 pKa = 3.92

Molecular weight: 14.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

15197

33

829

223.5

24.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.956 ± 0.414

0.98 ± 0.117

6.284 ± 0.173

5.573 ± 0.35

3.494 ± 0.182

7.482 ± 0.354

1.606 ± 0.169

6.179 ± 0.23

4.744 ± 0.295

7.587 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.161

4.718 ± 0.204

4.468 ± 0.234

3.988 ± 0.183

6.047 ± 0.253

6.804 ± 0.326

6.482 ± 0.279

6.6 ± 0.233

1.441 ± 0.12

2.994 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski