Roseburia sp. CAG:18

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; environmental samples

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2378 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5UKF7|R5UKF7_9FIRM Phosphoenolpyruvate-protein phosphotransferase OS=Roseburia sp. CAG:18 OX=1262941 GN=BN518_02038 PE=3 SV=1
MM1 pKa = 7.41KK2 pKa = 10.3KK3 pKa = 10.38KK4 pKa = 10.35IISTLLCTAMLATMVAGCGVEE25 pKa = 4.19KK26 pKa = 10.82SDD28 pKa = 5.1GGSDD32 pKa = 3.79TEE34 pKa = 4.74GSASSVAEE42 pKa = 3.98MKK44 pKa = 9.97GTGDD48 pKa = 3.46NEE50 pKa = 4.12INILIYY56 pKa = 10.74AQDD59 pKa = 3.59HH60 pKa = 6.05EE61 pKa = 4.52KK62 pKa = 10.74AVYY65 pKa = 9.89QEE67 pKa = 5.33LIDD70 pKa = 4.56KK71 pKa = 7.62FTKK74 pKa = 9.74EE75 pKa = 3.93HH76 pKa = 6.75ADD78 pKa = 3.76EE79 pKa = 4.53ISTVNFEE86 pKa = 3.94VTTQDD91 pKa = 2.86EE92 pKa = 5.24YY93 pKa = 9.91ATKK96 pKa = 8.37MTAAMTAGEE105 pKa = 4.84LPDD108 pKa = 3.48IFYY111 pKa = 10.84VGPEE115 pKa = 3.86AVRR118 pKa = 11.84SYY120 pKa = 11.46VDD122 pKa = 3.17NGYY125 pKa = 9.07VQPLDD130 pKa = 4.06DD131 pKa = 5.43LVDD134 pKa = 3.65ATAVDD139 pKa = 4.26NLWPAIKK146 pKa = 9.92SAYY149 pKa = 9.37MYY151 pKa = 10.28DD152 pKa = 3.37GSNIGSGSLYY162 pKa = 10.36CLPKK166 pKa = 10.68DD167 pKa = 3.88LSCFAFAYY175 pKa = 10.69NKK177 pKa = 10.62DD178 pKa = 3.75LFDD181 pKa = 4.1EE182 pKa = 5.31AGLEE186 pKa = 4.3YY187 pKa = 9.9PDD189 pKa = 5.09PEE191 pKa = 4.6NPYY194 pKa = 8.65TWEE197 pKa = 3.93EE198 pKa = 3.97FADD201 pKa = 3.88VCQKK205 pKa = 9.03LTKK208 pKa = 10.73DD209 pKa = 3.24KK210 pKa = 11.46DD211 pKa = 3.58GDD213 pKa = 4.06GEE215 pKa = 4.05IDD217 pKa = 3.06QWGVANANAFGFTPYY232 pKa = 10.64VYY234 pKa = 10.99GNGGQFLNDD243 pKa = 3.73DD244 pKa = 3.66QTKK247 pKa = 9.82VVIDD251 pKa = 3.74EE252 pKa = 4.14NQNFKK257 pKa = 11.09DD258 pKa = 3.19AFQYY262 pKa = 9.42YY263 pKa = 9.86TDD265 pKa = 3.96LTLKK269 pKa = 10.75YY270 pKa = 9.69KK271 pKa = 8.47VTPTVEE277 pKa = 3.57QDD279 pKa = 3.24TALGGYY285 pKa = 7.23QRR287 pKa = 11.84WLDD290 pKa = 3.8GQVGFYY296 pKa = 10.98ACGTWDD302 pKa = 3.1VAAFMDD308 pKa = 5.52DD309 pKa = 3.08ATFPYY314 pKa = 10.52NWDD317 pKa = 3.37LCGWPVGPNGDD328 pKa = 3.81GKK330 pKa = 9.44STAWLGTVGFAVSSQAKK347 pKa = 9.95NPEE350 pKa = 3.86LCAEE354 pKa = 5.45LIQSLSTDD362 pKa = 3.12LDD364 pKa = 3.99GQKK367 pKa = 9.85EE368 pKa = 4.43LCGEE372 pKa = 4.18TSGKK376 pKa = 9.97SLQIPNIMDD385 pKa = 3.53YY386 pKa = 11.33AQTTFKK392 pKa = 11.28DD393 pKa = 3.45KK394 pKa = 11.56VNDD397 pKa = 3.67GTIPYY402 pKa = 9.46ASNVDD407 pKa = 3.82VIFGYY412 pKa = 10.21IEE414 pKa = 4.88GSDD417 pKa = 3.38KK418 pKa = 11.3YY419 pKa = 11.0KK420 pKa = 11.16GIFTEE425 pKa = 4.1TTYY428 pKa = 10.53TYY430 pKa = 11.18NSEE433 pKa = 3.51WWDD436 pKa = 3.55TFLEE440 pKa = 4.46GMPNVLTGEE449 pKa = 4.2VSVDD453 pKa = 3.77DD454 pKa = 3.94YY455 pKa = 11.49CKK457 pKa = 10.11QVAPKK462 pKa = 9.07MQEE465 pKa = 3.89ALDD468 pKa = 3.95NAIEE472 pKa = 4.07LQNAATNNN480 pKa = 3.58

Molecular weight: 52.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5UKR8|R5UKR8_9FIRM ABC transmembrane type-1 domain-containing protein OS=Roseburia sp. CAG:18 OX=1262941 GN=BN518_02146 PE=3 SV=1
MM1 pKa = 7.51HH2 pKa = 7.22KK3 pKa = 10.29HH4 pKa = 5.1FGKK7 pKa = 8.85RR8 pKa = 11.84TKK10 pKa = 9.99KK11 pKa = 9.18KK12 pKa = 8.44HH13 pKa = 6.29HH14 pKa = 6.67IVLICSAVVIAAFVLRR30 pKa = 11.84MPVEE34 pKa = 4.11MNVADD39 pKa = 3.98ACGMQQKK46 pKa = 10.35VNVLSSSMHH55 pKa = 5.13NRR57 pKa = 11.84QAEE60 pKa = 4.33DD61 pKa = 3.62YY62 pKa = 10.53LNDD65 pKa = 3.78AGEE68 pKa = 4.25FCISQTVLRR77 pKa = 11.84EE78 pKa = 4.22GKK80 pKa = 9.59PSVVPQVRR88 pKa = 11.84RR89 pKa = 11.84IGLRR93 pKa = 11.84FLPVSHH99 pKa = 6.34MTQQWLLVQRR109 pKa = 11.84ILTSKK114 pKa = 10.41PKK116 pKa = 10.48FLVPPARR123 pKa = 11.84RR124 pKa = 11.84LIVGRR129 pKa = 11.84YY130 pKa = 7.6IRR132 pKa = 11.84ANPVCVV138 pKa = 3.24

Molecular weight: 15.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2378

0

2378

763030

30

1517

320.9

35.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.654 ± 0.05

1.491 ± 0.019

5.822 ± 0.046

7.312 ± 0.053

3.984 ± 0.035

6.995 ± 0.045

1.926 ± 0.022

7.119 ± 0.048

6.954 ± 0.047

8.693 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.238 ± 0.028

4.31 ± 0.04

3.201 ± 0.03

3.62 ± 0.034

4.286 ± 0.034

5.627 ± 0.041

5.61 ± 0.037

7.092 ± 0.042

0.893 ± 0.019

4.171 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski