Amino acid dipepetide frequency for Sporosarcina sp. HYO08

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.161AlaAla: 7.161 ± 0.123
0.63AlaCys: 0.63 ± 0.027
4.048AlaAsp: 4.048 ± 0.072
5.386AlaGlu: 5.386 ± 0.091
3.78AlaPhe: 3.78 ± 0.079
6.025AlaGly: 6.025 ± 0.091
1.505AlaHis: 1.505 ± 0.048
6.635AlaIle: 6.635 ± 0.107
4.857AlaLys: 4.857 ± 0.073
8.069AlaLeu: 8.069 ± 0.117
2.331AlaMet: 2.331 ± 0.054
2.925AlaAsn: 2.925 ± 0.062
2.344AlaPro: 2.344 ± 0.059
2.48AlaGln: 2.48 ± 0.058
2.951AlaArg: 2.951 ± 0.061
4.392AlaSer: 4.392 ± 0.079
4.037AlaThr: 4.037 ± 0.071
6.18AlaVal: 6.18 ± 0.097
0.611AlaTrp: 0.611 ± 0.026
2.495AlaTyr: 2.495 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
0.452CysAla: 0.452 ± 0.024
0.069CysCys: 0.069 ± 0.01
0.374CysAsp: 0.374 ± 0.02
0.412CysGlu: 0.412 ± 0.021
0.252CysPhe: 0.252 ± 0.017
0.707CysGly: 0.707 ± 0.025
0.176CysHis: 0.176 ± 0.013
0.485CysIle: 0.485 ± 0.021
0.293CysLys: 0.293 ± 0.024
0.551CysLeu: 0.551 ± 0.025
0.155CysMet: 0.155 ± 0.014
0.23CysAsn: 0.23 ± 0.016
0.345CysPro: 0.345 ± 0.019
0.198CysGln: 0.198 ± 0.017
0.263CysArg: 0.263 ± 0.017
0.438CysSer: 0.438 ± 0.022
0.417CysThr: 0.417 ± 0.023
0.388CysVal: 0.388 ± 0.022
0.062CysTrp: 0.062 ± 0.009
0.207CysTyr: 0.207 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
4.114AspAla: 4.114 ± 0.072
0.327AspCys: 0.327 ± 0.019
2.606AspAsp: 2.606 ± 0.075
4.649AspGlu: 4.649 ± 0.099
2.397AspPhe: 2.397 ± 0.063
3.649AspGly: 3.649 ± 0.062
1.272AspHis: 1.272 ± 0.036
3.862AspIle: 3.862 ± 0.072
2.434AspLys: 2.434 ± 0.054
4.981AspLeu: 4.981 ± 0.077
1.333AspMet: 1.333 ± 0.043
1.559AspAsn: 1.559 ± 0.052
2.256AspPro: 2.256 ± 0.061
2.173AspGln: 2.173 ± 0.053
2.54AspArg: 2.54 ± 0.059
2.319AspSer: 2.319 ± 0.059
2.392AspThr: 2.392 ± 0.049
4.173AspVal: 4.173 ± 0.076
0.569AspTrp: 0.569 ± 0.025
2.004AspTyr: 2.004 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
5.95GluAla: 5.95 ± 0.097
0.342GluCys: 0.342 ± 0.021
3.659GluAsp: 3.659 ± 0.076
6.996GluGlu: 6.996 ± 0.118
2.434GluPhe: 2.434 ± 0.059
4.626GluGly: 4.626 ± 0.083
1.399GluHis: 1.399 ± 0.038
5.398GluIle: 5.398 ± 0.09
6.191GluLys: 6.191 ± 0.106
6.968GluLeu: 6.968 ± 0.112
2.438GluMet: 2.438 ± 0.059
3.241GluAsn: 3.241 ± 0.059
2.07GluPro: 2.07 ± 0.057
3.838GluGln: 3.838 ± 0.093
3.902GluArg: 3.902 ± 0.07
3.519GluSer: 3.519 ± 0.064
4.128GluThr: 4.128 ± 0.076
5.247GluVal: 5.247 ± 0.09
0.833GluTrp: 0.833 ± 0.033
1.811GluTyr: 1.811 ± 0.046
0.0GluXaa: 0.0 ± 0.0
Phe
3.313PheAla: 3.313 ± 0.074
0.315PheCys: 0.315 ± 0.018
2.419PheAsp: 2.419 ± 0.055
2.926PheGlu: 2.926 ± 0.06
2.286PhePhe: 2.286 ± 0.077
3.6PheGly: 3.6 ± 0.068
1.057PheHis: 1.057 ± 0.038
3.697PheIle: 3.697 ± 0.071
2.181PheLys: 2.181 ± 0.058
4.389PheLeu: 4.389 ± 0.096
1.208PheMet: 1.208 ± 0.041
1.714PheAsn: 1.714 ± 0.048
1.699PhePro: 1.699 ± 0.045
1.524PheGln: 1.524 ± 0.042
1.698PheArg: 1.698 ± 0.046
3.137PheSer: 3.137 ± 0.064
2.818PheThr: 2.818 ± 0.06
3.352PheVal: 3.352 ± 0.068
0.438PheTrp: 0.438 ± 0.025
1.567PheTyr: 1.567 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
5.514GlyAla: 5.514 ± 0.084
0.598GlyCys: 0.598 ± 0.027
3.337GlyAsp: 3.337 ± 0.065
4.648GlyGlu: 4.648 ± 0.084
3.404GlyPhe: 3.404 ± 0.056
5.041GlyGly: 5.041 ± 0.105
1.482GlyHis: 1.482 ± 0.045
6.129GlyIle: 6.129 ± 0.092
5.163GlyLys: 5.163 ± 0.082
6.68GlyLeu: 6.68 ± 0.107
2.319GlyMet: 2.319 ± 0.062
2.792GlyAsn: 2.792 ± 0.06
1.786GlyPro: 1.786 ± 0.052
2.516GlyGln: 2.516 ± 0.076
2.979GlyArg: 2.979 ± 0.067
4.023GlySer: 4.023 ± 0.065
4.403GlyThr: 4.403 ± 0.072
5.292GlyVal: 5.292 ± 0.078
0.833GlyTrp: 0.833 ± 0.036
2.62GlyTyr: 2.62 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
1.633HisAla: 1.633 ± 0.045
0.178HisCys: 0.178 ± 0.013
1.027HisAsp: 1.027 ± 0.037
1.519HisGlu: 1.519 ± 0.046
1.035HisPhe: 1.035 ± 0.036
1.474HisGly: 1.474 ± 0.046
0.658HisHis: 0.658 ± 0.03
1.451HisIle: 1.451 ± 0.044
0.924HisLys: 0.924 ± 0.03
2.132HisLeu: 2.132 ± 0.046
0.546HisMet: 0.546 ± 0.026
0.68HisAsn: 0.68 ± 0.03
1.222HisPro: 1.222 ± 0.039
0.799HisGln: 0.799 ± 0.026
0.931HisArg: 0.931 ± 0.03
1.161HisSer: 1.161 ± 0.04
1.074HisThr: 1.074 ± 0.038
1.543HisVal: 1.543 ± 0.043
0.231HisTrp: 0.231 ± 0.015
0.861HisTyr: 0.861 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
7.071IleAla: 7.071 ± 0.109
0.55IleCys: 0.55 ± 0.025
4.519IleAsp: 4.519 ± 0.07
5.96IleGlu: 5.96 ± 0.09
3.193IlePhe: 3.193 ± 0.071
6.424IleGly: 6.424 ± 0.091
1.728IleHis: 1.728 ± 0.046
5.633IleIle: 5.633 ± 0.102
3.365IleLys: 3.365 ± 0.056
6.748IleLeu: 6.748 ± 0.108
1.727IleMet: 1.727 ± 0.047
2.588IleAsn: 2.588 ± 0.059
3.422IlePro: 3.422 ± 0.068
3.111IleGln: 3.111 ± 0.062
3.424IleArg: 3.424 ± 0.066
4.35IleSer: 4.35 ± 0.083
4.067IleThr: 4.067 ± 0.072
6.283IleVal: 6.283 ± 0.09
0.63IleTrp: 0.63 ± 0.026
2.21IleTyr: 2.21 ± 0.052
0.0IleXaa: 0.0 ± 0.0
Lys
4.487LysAla: 4.487 ± 0.071
0.277LysCys: 0.277 ± 0.017
3.383LysAsp: 3.383 ± 0.063
6.019LysGlu: 6.019 ± 0.107
1.91LysPhe: 1.91 ± 0.052
4.139LysGly: 4.139 ± 0.064
1.085LysHis: 1.085 ± 0.037
4.187LysIle: 4.187 ± 0.069
4.973LysLys: 4.973 ± 0.086
5.32LysLeu: 5.32 ± 0.086
2.274LysMet: 2.274 ± 0.052
2.944LysAsn: 2.944 ± 0.067
2.066LysPro: 2.066 ± 0.053
2.555LysGln: 2.555 ± 0.058
3.198LysArg: 3.198 ± 0.066
3.266LysSer: 3.266 ± 0.056
3.501LysThr: 3.501 ± 0.074
4.345LysVal: 4.345 ± 0.07
0.758LysTrp: 0.758 ± 0.032
1.793LysTyr: 1.793 ± 0.049
0.0LysXaa: 0.0 ± 0.0
Leu
7.923LeuAla: 7.923 ± 0.117
0.565LeuCys: 0.565 ± 0.025
4.775LeuAsp: 4.775 ± 0.075
6.316LeuGlu: 6.316 ± 0.1
4.974LeuPhe: 4.974 ± 0.109
6.46LeuGly: 6.46 ± 0.093
2.084LeuHis: 2.084 ± 0.054
6.923LeuIle: 6.923 ± 0.121
5.901LeuLys: 5.901 ± 0.088
10.279LeuLeu: 10.279 ± 0.151
2.541LeuMet: 2.541 ± 0.061
3.712LeuAsn: 3.712 ± 0.069
4.087LeuPro: 4.087 ± 0.074
3.752LeuGln: 3.752 ± 0.072
3.927LeuArg: 3.927 ± 0.075
6.349LeuSer: 6.349 ± 0.098
5.863LeuThr: 5.863 ± 0.076
6.312LeuVal: 6.312 ± 0.089
0.723LeuTrp: 0.723 ± 0.029
2.911LeuTyr: 2.911 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
2.219MetAla: 2.219 ± 0.051
0.125MetCys: 0.125 ± 0.014
1.542MetAsp: 1.542 ± 0.042
2.146MetGlu: 2.146 ± 0.056
1.014MetPhe: 1.014 ± 0.039
1.861MetGly: 1.861 ± 0.05
0.556MetHis: 0.556 ± 0.025
2.364MetIle: 2.364 ± 0.049
2.531MetLys: 2.531 ± 0.051
2.638MetLeu: 2.638 ± 0.061
0.956MetMet: 0.956 ± 0.038
1.484MetAsn: 1.484 ± 0.041
1.142MetPro: 1.142 ± 0.039
0.968MetGln: 0.968 ± 0.035
1.234MetArg: 1.234 ± 0.039
1.637MetSer: 1.637 ± 0.039
2.081MetThr: 2.081 ± 0.05
1.622MetVal: 1.622 ± 0.043
0.185MetTrp: 0.185 ± 0.012
0.743MetTyr: 0.743 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
3.012AsnAla: 3.012 ± 0.062
0.271AsnCys: 0.271 ± 0.018
2.178AsnAsp: 2.178 ± 0.054
3.307AsnGlu: 3.307 ± 0.058
1.593AsnPhe: 1.593 ± 0.051
3.395AsnGly: 3.395 ± 0.077
0.892AsnHis: 0.892 ± 0.033
2.877AsnIle: 2.877 ± 0.067
2.176AsnLys: 2.176 ± 0.055
3.353AsnLeu: 3.353 ± 0.069
1.087AsnMet: 1.087 ± 0.036
1.604AsnAsn: 1.604 ± 0.056
2.05AsnPro: 2.05 ± 0.05
1.362AsnGln: 1.362 ± 0.043
1.954AsnArg: 1.954 ± 0.051
1.887AsnSer: 1.887 ± 0.051
1.86AsnThr: 1.86 ± 0.046
2.951AsnVal: 2.951 ± 0.053
0.487AsnTrp: 0.487 ± 0.024
1.338AsnTyr: 1.338 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
2.784ProAla: 2.784 ± 0.057
0.223ProCys: 0.223 ± 0.017
2.021ProAsp: 2.021 ± 0.049
3.031ProGlu: 3.031 ± 0.061
2.062ProPhe: 2.062 ± 0.046
2.483ProGly: 2.483 ± 0.056
0.879ProHis: 0.879 ± 0.035
3.052ProIle: 3.052 ± 0.056
2.096ProLys: 2.096 ± 0.047
3.61ProLeu: 3.61 ± 0.076
0.947ProMet: 0.947 ± 0.034
1.602ProAsn: 1.602 ± 0.052
1.068ProPro: 1.068 ± 0.04
1.111ProGln: 1.111 ± 0.035
1.146ProArg: 1.146 ± 0.034
2.349ProSer: 2.349 ± 0.047
2.158ProThr: 2.158 ± 0.047
2.97ProVal: 2.97 ± 0.064
0.353ProTrp: 0.353 ± 0.018
1.392ProTyr: 1.392 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
3.011GlnAla: 3.011 ± 0.062
0.199GlnCys: 0.199 ± 0.017
1.661GlnAsp: 1.661 ± 0.048
2.728GlnGlu: 2.728 ± 0.063
1.756GlnPhe: 1.756 ± 0.046
1.989GlnGly: 1.989 ± 0.073
0.762GlnHis: 0.762 ± 0.027
2.532GlnIle: 2.532 ± 0.043
2.505GlnLys: 2.505 ± 0.057
4.182GlnLeu: 4.182 ± 0.084
1.258GlnMet: 1.258 ± 0.038
1.511GlnAsn: 1.511 ± 0.046
1.249GlnPro: 1.249 ± 0.038
1.93GlnGln: 1.93 ± 0.065
1.592GlnArg: 1.592 ± 0.052
2.205GlnSer: 2.205 ± 0.055
2.148GlnThr: 2.148 ± 0.052
2.325GlnVal: 2.325 ± 0.054
0.394GlnTrp: 0.394 ± 0.022
1.332GlnTyr: 1.332 ± 0.043
0.0GlnXaa: 0.0 ± 0.0
Arg
2.919ArgAla: 2.919 ± 0.061
0.251ArgCys: 0.251 ± 0.017
2.146ArgAsp: 2.146 ± 0.05
3.395ArgGlu: 3.395 ± 0.074
2.002ArgPhe: 2.002 ± 0.056
2.553ArgGly: 2.553 ± 0.058
0.948ArgHis: 0.948 ± 0.033
3.158ArgIle: 3.158 ± 0.067
3.392ArgLys: 3.392 ± 0.065
4.351ArgLeu: 4.351 ± 0.075
1.438ArgMet: 1.438 ± 0.038
1.868ArgAsn: 1.868 ± 0.044
1.498ArgPro: 1.498 ± 0.054
1.773ArgGln: 1.773 ± 0.051
2.011ArgArg: 2.011 ± 0.05
2.406ArgSer: 2.406 ± 0.046
2.379ArgThr: 2.379 ± 0.053
2.776ArgVal: 2.776 ± 0.063
0.396ArgTrp: 0.396 ± 0.023
1.459ArgTyr: 1.459 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
4.149SerAla: 4.149 ± 0.071
0.35SerCys: 0.35 ± 0.019
2.748SerAsp: 2.748 ± 0.061
3.637SerGlu: 3.637 ± 0.064
3.202SerPhe: 3.202 ± 0.058
4.506SerGly: 4.506 ± 0.076
1.127SerHis: 1.127 ± 0.036
4.864SerIle: 4.864 ± 0.078
3.419SerLys: 3.419 ± 0.072
5.664SerLeu: 5.664 ± 0.105
1.751SerMet: 1.751 ± 0.044
2.208SerAsn: 2.208 ± 0.058
2.168SerPro: 2.168 ± 0.049
1.735SerGln: 1.735 ± 0.05
2.391SerArg: 2.391 ± 0.049
3.41SerSer: 3.41 ± 0.081
3.101SerThr: 3.101 ± 0.062
4.118SerVal: 4.118 ± 0.071
0.535SerTrp: 0.535 ± 0.025
1.868SerTyr: 1.868 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
4.629ThrAla: 4.629 ± 0.072
0.344ThrCys: 0.344 ± 0.022
2.999ThrAsp: 2.999 ± 0.061
3.698ThrGlu: 3.698 ± 0.071
2.727ThrPhe: 2.727 ± 0.054
4.522ThrGly: 4.522 ± 0.08
1.094ThrHis: 1.094 ± 0.029
4.871ThrIle: 4.871 ± 0.086
3.174ThrLys: 3.174 ± 0.061
5.314ThrLeu: 5.314 ± 0.074
1.567ThrMet: 1.567 ± 0.048
2.323ThrAsn: 2.323 ± 0.057
2.393ThrPro: 2.393 ± 0.054
1.349ThrGln: 1.349 ± 0.035
2.001ThrArg: 2.001 ± 0.051
3.28ThrSer: 3.28 ± 0.052
3.176ThrThr: 3.176 ± 0.062
4.691ThrVal: 4.691 ± 0.083
0.468ThrTrp: 0.468 ± 0.024
1.889ThrTyr: 1.889 ± 0.052
0.0ThrXaa: 0.0 ± 0.0
Val
5.552ValAla: 5.552 ± 0.093
0.526ValCys: 0.526 ± 0.024
3.777ValAsp: 3.777 ± 0.07
5.085ValGlu: 5.085 ± 0.091
3.203ValPhe: 3.203 ± 0.061
4.954ValGly: 4.954 ± 0.079
1.51ValHis: 1.51 ± 0.04
5.838ValIle: 5.838 ± 0.097
4.521ValLys: 4.521 ± 0.069
7.082ValLeu: 7.082 ± 0.095
2.021ValMet: 2.021 ± 0.043
2.989ValAsn: 2.989 ± 0.062
2.873ValPro: 2.873 ± 0.06
2.624ValGln: 2.624 ± 0.055
3.017ValArg: 3.017 ± 0.058
4.435ValSer: 4.435 ± 0.072
4.49ValThr: 4.49 ± 0.079
5.398ValVal: 5.398 ± 0.098
0.594ValTrp: 0.594 ± 0.027
2.213ValTyr: 2.213 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
0.579TrpAla: 0.579 ± 0.025
0.076TrpCys: 0.076 ± 0.011
0.464TrpAsp: 0.464 ± 0.021
0.599TrpGlu: 0.599 ± 0.025
0.457TrpPhe: 0.457 ± 0.025
0.679TrpGly: 0.679 ± 0.034
0.216TrpHis: 0.216 ± 0.016
0.835TrpIle: 0.835 ± 0.032
0.649TrpLys: 0.649 ± 0.025
1.023TrpLeu: 1.023 ± 0.037
0.351TrpMet: 0.351 ± 0.02
0.441TrpAsn: 0.441 ± 0.021
0.276TrpPro: 0.276 ± 0.019
0.342TrpGln: 0.342 ± 0.018
0.42TrpArg: 0.42 ± 0.022
0.558TrpSer: 0.558 ± 0.027
0.603TrpThr: 0.603 ± 0.024
0.581TrpVal: 0.581 ± 0.026
0.11TrpTrp: 0.11 ± 0.012
0.281TrpTyr: 0.281 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.368TyrAla: 2.368 ± 0.053
0.259TyrCys: 0.259 ± 0.017
1.925TyrAsp: 1.925 ± 0.045
2.533TyrGlu: 2.533 ± 0.062
1.659TyrPhe: 1.659 ± 0.046
2.472TyrGly: 2.472 ± 0.059
0.651TyrHis: 0.651 ± 0.029
2.211TyrIle: 2.211 ± 0.051
1.737TyrLys: 1.737 ± 0.056
3.041TyrLeu: 3.041 ± 0.063
0.796TyrMet: 0.796 ± 0.033
1.219TyrAsn: 1.219 ± 0.043
1.323TyrPro: 1.323 ± 0.034
1.131TyrGln: 1.131 ± 0.038
1.54TyrArg: 1.54 ± 0.045
1.828TyrSer: 1.828 ± 0.044
1.812TyrThr: 1.812 ± 0.048
2.153TyrVal: 2.153 ± 0.051
0.333TyrTrp: 0.333 ± 0.02
1.15TyrTyr: 1.15 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2889 proteins (865762 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski