Gramella flava JLT2011

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Gramella; Gramella flava

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3628 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L7I558|A0A1L7I558_9FLAO Uncharacterized protein OS=Gramella flava JLT2011 OX=1229726 GN=GRFL_1521 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 9.17EE3 pKa = 3.66LKK5 pKa = 9.98MIFSLLVVLLMFSGCDD21 pKa = 3.45GDD23 pKa = 5.58EE24 pKa = 5.44DD25 pKa = 4.75DD26 pKa = 5.6LQLEE30 pKa = 4.61SMVVPTNIQIDD41 pKa = 4.05TDD43 pKa = 3.34ISTDD47 pKa = 3.0GSGIVVFDD55 pKa = 3.7VSADD59 pKa = 3.35NAITYY64 pKa = 9.36KK65 pKa = 10.77FNFGDD70 pKa = 3.81EE71 pKa = 4.48TTGTSLDD78 pKa = 3.23GTYY81 pKa = 9.88TKK83 pKa = 10.25RR84 pKa = 11.84YY85 pKa = 8.61SRR87 pKa = 11.84NGLNTYY93 pKa = 9.4QVLVVAYY100 pKa = 8.96GKK102 pKa = 11.0GGISSSKK109 pKa = 9.87RR110 pKa = 11.84IEE112 pKa = 3.87ISVEE116 pKa = 3.82SDD118 pKa = 3.09FSDD121 pKa = 4.27PEE123 pKa = 4.19TKK125 pKa = 10.36EE126 pKa = 3.72FLTGGSSKK134 pKa = 8.61MWYY137 pKa = 9.12VAAFQPGHH145 pKa = 6.78LGVGPSSGDD154 pKa = 3.4GFDD157 pKa = 3.44SPIYY161 pKa = 10.08YY162 pKa = 10.08SAAPFEE168 pKa = 4.41KK169 pKa = 10.56AGAEE173 pKa = 3.84ASACFYY179 pKa = 10.93TDD181 pKa = 2.79VLTFSTTGEE190 pKa = 4.0NNFTYY195 pKa = 10.93NLNNNGATFFNVDD208 pKa = 3.17YY209 pKa = 10.42VGQFGGPTGSGDD221 pKa = 3.14QCIAYY226 pKa = 7.75DD227 pKa = 3.52TGGEE231 pKa = 4.18KK232 pKa = 9.6TVTLSPATSGIEE244 pKa = 4.12SSTGTVMDD252 pKa = 4.85FSDD255 pKa = 4.17GGFMSYY261 pKa = 11.04YY262 pKa = 9.75IGTSTYY268 pKa = 10.18EE269 pKa = 3.68ILDD272 pKa = 3.26IDD274 pKa = 4.43EE275 pKa = 4.3NSMYY279 pKa = 10.47VRR281 pKa = 11.84AIMGNNPALAWYY293 pKa = 9.91LKK295 pKa = 9.58FTTTPYY301 pKa = 10.68DD302 pKa = 3.49QQQEE306 pKa = 4.35SGNGGEE312 pKa = 4.47EE313 pKa = 3.75EE314 pKa = 5.37AEE316 pKa = 4.81FEE318 pKa = 4.5TQFNNLVWSDD328 pKa = 3.85EE329 pKa = 4.03FDD331 pKa = 4.12GDD333 pKa = 5.44SLDD336 pKa = 3.58TDD338 pKa = 3.14SWNFEE343 pKa = 4.33TGNGTNGWGNNEE355 pKa = 3.83LQYY358 pKa = 11.41YY359 pKa = 9.18LQDD362 pKa = 3.25NVTVQDD368 pKa = 3.52GSLFITAKK376 pKa = 10.29RR377 pKa = 11.84EE378 pKa = 4.17SEE380 pKa = 4.13SGFDD384 pKa = 3.58FTSGRR389 pKa = 11.84ITTQDD394 pKa = 3.06KK395 pKa = 11.46YY396 pKa = 9.91EE397 pKa = 3.96FTYY400 pKa = 10.82GRR402 pKa = 11.84VEE404 pKa = 3.95ARR406 pKa = 11.84IKK408 pKa = 10.75LPEE411 pKa = 4.45GVGTWPAFWMLGANFDD427 pKa = 3.94GVGWPEE433 pKa = 3.77TGEE436 pKa = 3.81IDD438 pKa = 3.19ILEE441 pKa = 4.41WVGKK445 pKa = 9.51EE446 pKa = 3.61PGRR449 pKa = 11.84IQSALHH455 pKa = 5.72FPGNSGGNAVVGPSTIEE472 pKa = 3.89NPSSEE477 pKa = 4.11FHH479 pKa = 6.74TYY481 pKa = 7.12TAEE484 pKa = 3.88WTEE487 pKa = 3.89DD488 pKa = 3.77AITFLLDD495 pKa = 3.11GEE497 pKa = 5.1VYY499 pKa = 8.92FTFDD503 pKa = 3.7NDD505 pKa = 3.22SDD507 pKa = 4.08KK508 pKa = 11.32PFNKK512 pKa = 10.21DD513 pKa = 2.79FFLILNVAMGGNLGGDD529 pKa = 3.35VDD531 pKa = 4.21PAFSEE536 pKa = 4.42SAMEE540 pKa = 3.65IDD542 pKa = 3.89YY543 pKa = 11.43VKK545 pKa = 10.7VYY547 pKa = 8.84QQ548 pKa = 4.21

Molecular weight: 59.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L7I2D2|A0A1L7I2D2_9FLAO Uncharacterized protein OS=Gramella flava JLT2011 OX=1229726 GN=GRFL_1017 PE=4 SV=1
MM1 pKa = 7.48SKK3 pKa = 9.86YY4 pKa = 10.32FRR6 pKa = 11.84YY7 pKa = 9.84FEE9 pKa = 3.78YY10 pKa = 10.54AYY12 pKa = 10.61LIFAAFFLFEE22 pKa = 5.43AVRR25 pKa = 11.84IWNSEE30 pKa = 3.55RR31 pKa = 11.84SRR33 pKa = 11.84AYY35 pKa = 9.41IFLFFVAIAVFMFFFKK51 pKa = 10.56RR52 pKa = 11.84RR53 pKa = 11.84FRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84FEE59 pKa = 4.08EE60 pKa = 4.04RR61 pKa = 11.84NKK63 pKa = 11.0

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3628

0

3628

1223118

37

4291

337.1

38.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.605 ± 0.04

0.693 ± 0.019

5.715 ± 0.05

7.429 ± 0.039

5.211 ± 0.034

6.663 ± 0.043

1.81 ± 0.021

7.31 ± 0.038

7.08 ± 0.062

9.302 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.358 ± 0.026

5.595 ± 0.04

3.597 ± 0.024

3.788 ± 0.026

3.952 ± 0.032

6.519 ± 0.037

5.2 ± 0.058

6.041 ± 0.033

1.118 ± 0.016

4.015 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski