Tetraselmis viridis virus S1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified dsDNA viruses

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|M4QR70|M4QR70_9VIRU Uncharacterized protein OS=Tetraselmis viridis virus S1 OX=756285 GN=TVSG_00009 PE=4 SV=1
MM1 pKa = 7.16ATIGTRR7 pKa = 11.84AFLATDD13 pKa = 4.31GNWQRR18 pKa = 11.84QYY20 pKa = 11.7DD21 pKa = 4.03NNGNRR26 pKa = 11.84IDD28 pKa = 4.47SIWVPGNNPRR38 pKa = 11.84EE39 pKa = 4.12VNNRR43 pKa = 11.84YY44 pKa = 7.71LTRR47 pKa = 11.84DD48 pKa = 2.99RR49 pKa = 11.84NMRR52 pKa = 11.84AVGAAILPRR61 pKa = 11.84EE62 pKa = 4.14IQDD65 pKa = 4.05KK66 pKa = 10.58IEE68 pKa = 3.97MMALEE73 pKa = 4.93LKK75 pKa = 10.37SDD77 pKa = 3.36QLTAEE82 pKa = 4.07RR83 pKa = 11.84LEE85 pKa = 4.09YY86 pKa = 11.05LNDD89 pKa = 3.11EE90 pKa = 4.22ATPVNEE96 pKa = 4.24EE97 pKa = 3.91VEE99 pKa = 4.3EE100 pKa = 4.31LSDD103 pKa = 3.63YY104 pKa = 11.5DD105 pKa = 3.64KK106 pKa = 11.52DD107 pKa = 3.92VIYY110 pKa = 10.51GHH112 pKa = 6.33VYY114 pKa = 10.86EE115 pKa = 6.09NFDD118 pKa = 3.3LWFSEE123 pKa = 4.16PEE125 pKa = 4.0YY126 pKa = 10.41PEE128 pKa = 3.89WSPTLIEE135 pKa = 4.78FLRR138 pKa = 11.84QNDD141 pKa = 3.71FNYY144 pKa = 10.72DD145 pKa = 3.2EE146 pKa = 4.7YY147 pKa = 11.06EE148 pKa = 3.93ITEE151 pKa = 4.25EE152 pKa = 4.29LPSVIDD158 pKa = 4.17GIYY161 pKa = 9.6IGRR164 pKa = 11.84NVIYY168 pKa = 10.36GRR170 pKa = 11.84YY171 pKa = 7.99LHH173 pKa = 6.93KK174 pKa = 10.8FQDD177 pKa = 5.0DD178 pKa = 4.22DD179 pKa = 4.25FCDD182 pKa = 5.28DD183 pKa = 3.85IQNLAANLGVTLHH196 pKa = 5.98TWADD200 pKa = 2.9IDD202 pKa = 4.25SFCKK206 pKa = 9.99NVYY209 pKa = 10.11IPVALILPPILINDD223 pKa = 3.89DD224 pKa = 4.6PNWGMDD230 pKa = 3.63DD231 pKa = 5.12DD232 pKa = 4.51EE233 pKa = 6.4VIDD236 pKa = 5.51LVSDD240 pKa = 3.94DD241 pKa = 4.94DD242 pKa = 3.82ATIIISDD249 pKa = 3.96SDD251 pKa = 4.16DD252 pKa = 3.49EE253 pKa = 4.42PMM255 pKa = 5.24

Molecular weight:
29.56 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|M4QR85|M4QR85_9VIRU Uncharacterized protein OS=Tetraselmis viridis virus S1 OX=756285 GN=TVSG_00024 PE=4 SV=1
MM1 pKa = 7.79IIGAKK6 pKa = 9.85SGAHH10 pKa = 6.04SGLSAVIGSKK20 pKa = 10.63NNNSHH25 pKa = 4.97MTMGSKK31 pKa = 10.49GGAPSHH37 pKa = 6.77HH38 pKa = 6.07GRR40 pKa = 11.84AVYY43 pKa = 8.49TQANRR48 pKa = 11.84AMGDD52 pKa = 3.17MGGGKK57 pKa = 9.16SAPGNTHH64 pKa = 6.25NSVGGGQRR72 pKa = 11.84DD73 pKa = 3.61APSRR77 pKa = 11.84PDD79 pKa = 4.18GIRR82 pKa = 11.84SMADD86 pKa = 2.92PRR88 pKa = 11.84PSKK91 pKa = 10.14RR92 pKa = 11.84AKK94 pKa = 7.47TTEE97 pKa = 3.81SGKK100 pKa = 10.45KK101 pKa = 9.82NKK103 pKa = 10.38AEE105 pKa = 3.88

Molecular weight:
10.64 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

8262

88

1274

344.3

38.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.826 ± 0.421

1.041 ± 0.264

7.649 ± 0.474

6.258 ± 0.381

3.546 ± 0.258

7.25 ± 0.497

2.481 ± 0.203

5.507 ± 0.283

4.999 ± 0.568

6.705 ± 0.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.784 ± 0.331

5.277 ± 0.386

5.507 ± 0.407

3.002 ± 0.251

5.894 ± 0.51

7.286 ± 0.418

6.536 ± 0.575

6.245 ± 0.395

1.198 ± 0.167

4.006 ± 0.348

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski