Tetraselmis viridis virus S1
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M4QR70|M4QR70_9VIRU Uncharacterized protein OS=Tetraselmis viridis virus S1 OX=756285 GN=TVSG_00009 PE=4 SV=1
MM1 pKa = 7.16 ATIGTRR7 pKa = 11.84 AFLATDD13 pKa = 4.31 GNWQRR18 pKa = 11.84 QYY20 pKa = 11.7 DD21 pKa = 4.03 NNGNRR26 pKa = 11.84 IDD28 pKa = 4.47 SIWVPGNNPRR38 pKa = 11.84 EE39 pKa = 4.12 VNNRR43 pKa = 11.84 YY44 pKa = 7.71 LTRR47 pKa = 11.84 DD48 pKa = 2.99 RR49 pKa = 11.84 NMRR52 pKa = 11.84 AVGAAILPRR61 pKa = 11.84 EE62 pKa = 4.14 IQDD65 pKa = 4.05 KK66 pKa = 10.58 IEE68 pKa = 3.97 MMALEE73 pKa = 4.93 LKK75 pKa = 10.37 SDD77 pKa = 3.36 QLTAEE82 pKa = 4.07 RR83 pKa = 11.84 LEE85 pKa = 4.09 YY86 pKa = 11.05 LNDD89 pKa = 3.11 EE90 pKa = 4.22 ATPVNEE96 pKa = 4.24 EE97 pKa = 3.91 VEE99 pKa = 4.3 EE100 pKa = 4.31 LSDD103 pKa = 3.63 YY104 pKa = 11.5 DD105 pKa = 3.64 KK106 pKa = 11.52 DD107 pKa = 3.92 VIYY110 pKa = 10.51 GHH112 pKa = 6.33 VYY114 pKa = 10.86 EE115 pKa = 6.09 NFDD118 pKa = 3.3 LWFSEE123 pKa = 4.16 PEE125 pKa = 4.0 YY126 pKa = 10.41 PEE128 pKa = 3.89 WSPTLIEE135 pKa = 4.78 FLRR138 pKa = 11.84 QNDD141 pKa = 3.71 FNYY144 pKa = 10.72 DD145 pKa = 3.2 EE146 pKa = 4.7 YY147 pKa = 11.06 EE148 pKa = 3.93 ITEE151 pKa = 4.25 EE152 pKa = 4.29 LPSVIDD158 pKa = 4.17 GIYY161 pKa = 9.6 IGRR164 pKa = 11.84 NVIYY168 pKa = 10.36 GRR170 pKa = 11.84 YY171 pKa = 7.99 LHH173 pKa = 6.93 KK174 pKa = 10.8 FQDD177 pKa = 5.0 DD178 pKa = 4.22 DD179 pKa = 4.25 FCDD182 pKa = 5.28 DD183 pKa = 3.85 IQNLAANLGVTLHH196 pKa = 5.98 TWADD200 pKa = 2.9 IDD202 pKa = 4.25 SFCKK206 pKa = 9.99 NVYY209 pKa = 10.11 IPVALILPPILINDD223 pKa = 3.89 DD224 pKa = 4.6 PNWGMDD230 pKa = 3.63 DD231 pKa = 5.12 DD232 pKa = 4.51 EE233 pKa = 6.4 VIDD236 pKa = 5.51 LVSDD240 pKa = 3.94 DD241 pKa = 4.94 DD242 pKa = 3.82 ATIIISDD249 pKa = 3.96 SDD251 pKa = 4.16 DD252 pKa = 3.49 EE253 pKa = 4.42 PMM255 pKa = 5.24
Molecular weight: 29.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.021
IPC2_protein 3.846
IPC_protein 3.859
Toseland 3.643
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.681
Grimsley 3.541
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.19
Thurlkill 3.681
EMBOSS 3.783
Sillero 3.973
Patrickios 3.083
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.896
Protein with the highest isoelectric point:
>tr|M4QR85|M4QR85_9VIRU Uncharacterized protein OS=Tetraselmis viridis virus S1 OX=756285 GN=TVSG_00024 PE=4 SV=1
MM1 pKa = 7.79 IIGAKK6 pKa = 9.85 SGAHH10 pKa = 6.04 SGLSAVIGSKK20 pKa = 10.63 NNNSHH25 pKa = 4.97 MTMGSKK31 pKa = 10.49 GGAPSHH37 pKa = 6.77 HH38 pKa = 6.07 GRR40 pKa = 11.84 AVYY43 pKa = 8.49 TQANRR48 pKa = 11.84 AMGDD52 pKa = 3.17 MGGGKK57 pKa = 9.16 SAPGNTHH64 pKa = 6.25 NSVGGGQRR72 pKa = 11.84 DD73 pKa = 3.61 APSRR77 pKa = 11.84 PDD79 pKa = 4.18 GIRR82 pKa = 11.84 SMADD86 pKa = 2.92 PRR88 pKa = 11.84 PSKK91 pKa = 10.14 RR92 pKa = 11.84 AKK94 pKa = 7.47 TTEE97 pKa = 3.81 SGKK100 pKa = 10.45 KK101 pKa = 9.82 NKK103 pKa = 10.38 AEE105 pKa = 3.88
Molecular weight: 10.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.257
IPC2_protein 9.809
IPC_protein 10.365
Toseland 11.082
ProMoST 10.701
Dawson 11.125
Bjellqvist 10.789
Wikipedia 11.301
Rodwell 11.506
Grimsley 11.14
Solomon 11.272
Lehninger 11.242
Nozaki 11.038
DTASelect 10.789
Thurlkill 11.052
EMBOSS 11.477
Sillero 11.052
Patrickios 11.257
IPC_peptide 11.286
IPC2_peptide 9.311
IPC2.peptide.svr19 8.718
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24
0
24
8262
88
1274
344.3
38.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.826 ± 0.421
1.041 ± 0.264
7.649 ± 0.474
6.258 ± 0.381
3.546 ± 0.258
7.25 ± 0.497
2.481 ± 0.203
5.507 ± 0.283
4.999 ± 0.568
6.705 ± 0.371
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.784 ± 0.331
5.277 ± 0.386
5.507 ± 0.407
3.002 ± 0.251
5.894 ± 0.51
7.286 ± 0.418
6.536 ± 0.575
6.245 ± 0.395
1.198 ± 0.167
4.006 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here