Amino acid dipepetide frequency for Bacillus sp. 7586-K

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.619AlaAla: 4.619 ± 0.086	0.539AlaCys: 0.539 ± 0.019	2.968AlaAsp: 2.968 ± 0.048	4.307AlaGlu: 4.307 ± 0.055	2.992AlaPhe: 2.992 ± 0.054	4.356AlaGly: 4.356 ± 0.074	1.086AlaHis: 1.086 ± 0.028	5.999AlaIle: 5.999 ± 0.075	4.752AlaLys: 4.752 ± 0.059	6.359AlaLeu: 6.359 ± 0.081	1.692AlaMet: 1.692 ± 0.036	3.054AlaAsn: 3.054 ± 0.051	1.796AlaPro: 1.796 ± 0.045	1.926AlaGln: 1.926 ± 0.04	2.2AlaArg: 2.2 ± 0.044	3.837AlaSer: 3.837 ± 0.06	3.329AlaThr: 3.329 ± 0.057	4.457AlaVal: 4.457 ± 0.06	0.561AlaTrp: 0.561 ± 0.024	2.159AlaTyr: 2.159 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.375CysAla: 0.375 ± 0.018	0.112CysCys: 0.112 ± 0.01	0.36CysAsp: 0.36 ± 0.014	0.53CysGlu: 0.53 ± 0.023	0.356CysPhe: 0.356 ± 0.015	0.605CysGly: 0.605 ± 0.023	0.217CysHis: 0.217 ± 0.013	0.612CysIle: 0.612 ± 0.021	0.425CysLys: 0.425 ± 0.018	0.743CysLeu: 0.743 ± 0.02	0.172CysMet: 0.172 ± 0.011	0.341CysAsn: 0.341 ± 0.014	0.313CysPro: 0.313 ± 0.017	0.237CysGln: 0.237 ± 0.013	0.269CysArg: 0.269 ± 0.013	0.515CysSer: 0.515 ± 0.018	0.38CysThr: 0.38 ± 0.016	0.374CysVal: 0.374 ± 0.016	0.083CysTrp: 0.083 ± 0.007	0.275CysTyr: 0.275 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	2.95AspAla: 2.95 ± 0.049	0.372AspCys: 0.372 ± 0.017	2.613AspAsp: 2.613 ± 0.045	4.735AspGlu: 4.735 ± 0.062	2.607AspPhe: 2.607 ± 0.042	3.332AspGly: 3.332 ± 0.05	1.031AspHis: 1.031 ± 0.029	4.234AspIle: 4.234 ± 0.053	3.47AspLys: 3.47 ± 0.051	4.919AspLeu: 4.919 ± 0.056	1.246AspMet: 1.246 ± 0.029	2.137AspAsn: 2.137 ± 0.042	1.876AspPro: 1.876 ± 0.048	1.567AspGln: 1.567 ± 0.034	1.914AspArg: 1.914 ± 0.033	2.669AspSer: 2.669 ± 0.041	2.432AspThr: 2.432 ± 0.043	3.63AspVal: 3.63 ± 0.06	0.653AspTrp: 0.653 ± 0.025	2.34AspTyr: 2.34 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.13GluAla: 5.13 ± 0.068	0.417GluCys: 0.417 ± 0.018	3.861GluAsp: 3.861 ± 0.057	7.178GluGlu: 7.178 ± 0.09	2.772GluPhe: 2.772 ± 0.046	4.114GluGly: 4.114 ± 0.058	1.624GluHis: 1.624 ± 0.031	6.314GluIle: 6.314 ± 0.064	6.973GluLys: 6.973 ± 0.079	7.323GluLeu: 7.323 ± 0.076	2.265GluMet: 2.265 ± 0.038	4.292GluAsn: 4.292 ± 0.064	1.863GluPro: 1.863 ± 0.037	3.705GluGln: 3.705 ± 0.062	3.352GluArg: 3.352 ± 0.052	3.485GluSer: 3.485 ± 0.052	3.888GluThr: 3.888 ± 0.062	4.977GluVal: 4.977 ± 0.071	0.857GluTrp: 0.857 ± 0.023	2.571GluTyr: 2.571 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	2.845PheAla: 2.845 ± 0.052	0.374PheCys: 0.374 ± 0.017	2.451PheAsp: 2.451 ± 0.043	3.023PheGlu: 3.023 ± 0.046	2.421PhePhe: 2.421 ± 0.049	3.175PheGly: 3.175 ± 0.05	1.137PheHis: 1.137 ± 0.031	4.484PheIle: 4.484 ± 0.064	2.697PheLys: 2.697 ± 0.041	4.745PheLeu: 4.745 ± 0.055	1.139PheMet: 1.139 ± 0.027	2.113PheAsn: 2.113 ± 0.042	1.567PhePro: 1.567 ± 0.032	1.684PheGln: 1.684 ± 0.036	1.431PheArg: 1.431 ± 0.032	3.414PheSer: 3.414 ± 0.042	2.709PheThr: 2.709 ± 0.046	3.082PheVal: 3.082 ± 0.055	0.513PheTrp: 0.513 ± 0.021	1.85PheTyr: 1.85 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	4.104GlyAla: 4.104 ± 0.072	0.607GlyCys: 0.607 ± 0.024	3.003GlyAsp: 3.003 ± 0.049	4.47GlyGlu: 4.47 ± 0.062	3.305GlyPhe: 3.305 ± 0.055	4.318GlyGly: 4.318 ± 0.076	1.26GlyHis: 1.26 ± 0.034	5.844GlyIle: 5.844 ± 0.077	4.959GlyLys: 4.959 ± 0.061	6.281GlyLeu: 6.281 ± 0.072	1.896GlyMet: 1.896 ± 0.035	2.938GlyAsn: 2.938 ± 0.051	1.561GlyPro: 1.561 ± 0.035	1.996GlyGln: 1.996 ± 0.035	2.321GlyArg: 2.321 ± 0.041	3.646GlySer: 3.646 ± 0.05	3.81GlyThr: 3.81 ± 0.06	4.505GlyVal: 4.505 ± 0.063	0.792GlyTrp: 0.792 ± 0.026	2.836GlyTyr: 2.836 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	1.173HisAla: 1.173 ± 0.029	0.204HisCys: 0.204 ± 0.012	1.068HisAsp: 1.068 ± 0.029	1.386HisGlu: 1.386 ± 0.029	1.132HisPhe: 1.132 ± 0.031	1.287HisGly: 1.287 ± 0.03	0.729HisHis: 0.729 ± 0.026	1.631HisIle: 1.631 ± 0.033	1.098HisLys: 1.098 ± 0.024	2.096HisLeu: 2.096 ± 0.04	0.455HisMet: 0.455 ± 0.017	0.87HisAsn: 0.87 ± 0.026	1.039HisPro: 1.039 ± 0.028	0.829HisGln: 0.829 ± 0.026	0.816HisArg: 0.816 ± 0.025	1.263HisSer: 1.263 ± 0.034	1.054HisThr: 1.054 ± 0.029	1.387HisVal: 1.387 ± 0.03	0.243HisTrp: 0.243 ± 0.014	1.011HisTyr: 1.011 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.873IleAla: 5.873 ± 0.077	0.752IleCys: 0.752 ± 0.021	4.814IleAsp: 4.814 ± 0.058	6.509IleGlu: 6.509 ± 0.071	3.682IlePhe: 3.682 ± 0.054	6.072IleGly: 6.072 ± 0.079	1.738IleHis: 1.738 ± 0.036	6.949IleIle: 6.949 ± 0.093	5.489IleLys: 5.489 ± 0.064	7.481IleLeu: 7.481 ± 0.083	1.815IleMet: 1.815 ± 0.035	3.929IleAsn: 3.929 ± 0.059	3.395IlePro: 3.395 ± 0.049	3.061IleGln: 3.061 ± 0.048	3.111IleArg: 3.111 ± 0.047	5.618IleSer: 5.618 ± 0.071	4.744IleThr: 4.744 ± 0.058	5.949IleVal: 5.949 ± 0.068	0.744IleTrp: 0.744 ± 0.026	2.86IleTyr: 2.86 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	4.463LysAla: 4.463 ± 0.059	0.369LysCys: 0.369 ± 0.016	4.036LysAsp: 4.036 ± 0.054	7.291LysGlu: 7.291 ± 0.078	2.198LysPhe: 2.198 ± 0.04	4.609LysGly: 4.609 ± 0.058	1.492LysHis: 1.492 ± 0.031	5.368LysIle: 5.368 ± 0.065	6.118LysLys: 6.118 ± 0.075	6.52LysLeu: 6.52 ± 0.068	2.202LysMet: 2.202 ± 0.038	3.711LysAsn: 3.711 ± 0.051	2.298LysPro: 2.298 ± 0.042	3.561LysGln: 3.561 ± 0.052	3.404LysArg: 3.404 ± 0.06	3.863LysSer: 3.863 ± 0.054	3.838LysThr: 3.838 ± 0.045	4.759LysVal: 4.759 ± 0.065	0.892LysTrp: 0.892 ± 0.022	2.565LysTyr: 2.565 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	6.62LeuAla: 6.62 ± 0.086	0.733LeuCys: 0.733 ± 0.024	4.682LeuAsp: 4.682 ± 0.057	6.63LeuGlu: 6.63 ± 0.076	5.061LeuPhe: 5.061 ± 0.078	5.921LeuGly: 5.921 ± 0.081	1.97LeuHis: 1.97 ± 0.039	7.861LeuIle: 7.861 ± 0.082	7.135LeuLys: 7.135 ± 0.07	10.258LeuLeu: 10.258 ± 0.114	2.372LeuMet: 2.372 ± 0.044	4.792LeuAsn: 4.792 ± 0.055	3.72LeuPro: 3.72 ± 0.051	3.586LeuGln: 3.586 ± 0.05	3.266LeuArg: 3.266 ± 0.052	6.935LeuSer: 6.935 ± 0.074	5.773LeuThr: 5.773 ± 0.066	5.958LeuVal: 5.958 ± 0.073	0.853LeuTrp: 0.853 ± 0.025	3.382LeuTyr: 3.382 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	1.661MetAla: 1.661 ± 0.034	0.141MetCys: 0.141 ± 0.009	1.309MetAsp: 1.309 ± 0.034	1.796MetGlu: 1.796 ± 0.034	1.076MetPhe: 1.076 ± 0.03	1.51MetGly: 1.51 ± 0.033	0.352MetHis: 0.352 ± 0.017	2.257MetIle: 2.257 ± 0.043	2.595MetLys: 2.595 ± 0.039	2.396MetLeu: 2.396 ± 0.041	0.831MetMet: 0.831 ± 0.026	1.617MetAsn: 1.617 ± 0.033	0.887MetPro: 0.887 ± 0.026	0.795MetGln: 0.795 ± 0.023	0.964MetArg: 0.964 ± 0.026	1.679MetSer: 1.679 ± 0.031	1.54MetThr: 1.54 ± 0.029	1.578MetVal: 1.578 ± 0.034	0.202MetTrp: 0.202 ± 0.011	0.745MetTyr: 0.745 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.601AsnAla: 2.601 ± 0.044	0.339AsnCys: 0.339 ± 0.016	2.589AsnAsp: 2.589 ± 0.045	4.411AsnGlu: 4.411 ± 0.059	1.787AsnPhe: 1.787 ± 0.034	3.834AsnGly: 3.834 ± 0.052	1.209AsnHis: 1.209 ± 0.029	3.77AsnIle: 3.77 ± 0.053	3.538AsnLys: 3.538 ± 0.053	4.099AsnLeu: 4.099 ± 0.052	1.213AsnMet: 1.213 ± 0.031	2.402AsnAsn: 2.402 ± 0.048	2.175AsnPro: 2.175 ± 0.045	2.196AsnGln: 2.196 ± 0.047	2.199AsnArg: 2.199 ± 0.04	2.779AsnSer: 2.779 ± 0.041	2.353AsnThr: 2.353 ± 0.038	3.273AsnVal: 3.273 ± 0.045	0.641AsnTrp: 0.641 ± 0.022	1.869AsnTyr: 1.869 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	1.901ProAla: 1.901 ± 0.037	0.187ProCys: 0.187 ± 0.011	1.838ProAsp: 1.838 ± 0.043	2.728ProGlu: 2.728 ± 0.057	1.985ProPhe: 1.985 ± 0.038	1.968ProGly: 1.968 ± 0.044	0.749ProHis: 0.749 ± 0.024	3.071ProIle: 3.071 ± 0.046	2.243ProLys: 2.243 ± 0.039	3.287ProLeu: 3.287 ± 0.05	0.724ProMet: 0.724 ± 0.025	1.891ProAsn: 1.891 ± 0.036	0.883ProPro: 0.883 ± 0.026	1.024ProGln: 1.024 ± 0.024	0.955ProArg: 0.955 ± 0.028	2.239ProSer: 2.239 ± 0.033	2.009ProThr: 2.009 ± 0.041	2.325ProVal: 2.325 ± 0.044	0.396ProTrp: 0.396 ± 0.016	1.467ProTyr: 1.467 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	2.479GlnAla: 2.479 ± 0.043	0.196GlnCys: 0.196 ± 0.01	1.57GlnAsp: 1.57 ± 0.034	2.652GlnGlu: 2.652 ± 0.048	1.808GlnPhe: 1.808 ± 0.035	2.026GlnGly: 2.026 ± 0.034	0.785GlnHis: 0.785 ± 0.024	2.991GlnIle: 2.991 ± 0.047	2.739GlnLys: 2.739 ± 0.046	4.239GlnLeu: 4.239 ± 0.059	1.056GlnMet: 1.056 ± 0.029	1.736GlnAsn: 1.736 ± 0.032	1.131GlnPro: 1.131 ± 0.032	1.814GlnGln: 1.814 ± 0.044	1.349GlnArg: 1.349 ± 0.034	2.207GlnSer: 2.207 ± 0.044	1.955GlnThr: 1.955 ± 0.035	2.306GlnVal: 2.306 ± 0.037	0.414GlnTrp: 0.414 ± 0.017	1.478GlnTyr: 1.478 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	2.136ArgAla: 2.136 ± 0.043	0.23ArgCys: 0.23 ± 0.016	1.882ArgAsp: 1.882 ± 0.037	3.074ArgGlu: 3.074 ± 0.05	1.876ArgPhe: 1.876 ± 0.042	2.063ArgGly: 2.063 ± 0.039	0.712ArgHis: 0.712 ± 0.022	3.07ArgIle: 3.07 ± 0.048	3.198ArgLys: 3.198 ± 0.056	3.765ArgLeu: 3.765 ± 0.056	1.089ArgMet: 1.089 ± 0.029	2.022ArgAsn: 2.022 ± 0.036	1.154ArgPro: 1.154 ± 0.029	1.362ArgGln: 1.362 ± 0.029	1.65ArgArg: 1.65 ± 0.037	2.008ArgSer: 2.008 ± 0.042	1.897ArgThr: 1.897 ± 0.035	2.338ArgVal: 2.338 ± 0.04	0.395ArgTrp: 0.395 ± 0.018	1.513ArgTyr: 1.513 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	3.465SerAla: 3.465 ± 0.052	0.412SerCys: 0.412 ± 0.016	2.821SerAsp: 2.821 ± 0.048	4.255SerGlu: 4.255 ± 0.056	3.474SerPhe: 3.474 ± 0.051	4.084SerGly: 4.084 ± 0.058	1.197SerHis: 1.197 ± 0.026	5.682SerIle: 5.682 ± 0.068	4.242SerLys: 4.242 ± 0.054	6.395SerLeu: 6.395 ± 0.073	1.644SerMet: 1.644 ± 0.034	2.965SerAsn: 2.965 ± 0.049	2.012SerPro: 2.012 ± 0.036	2.015SerGln: 2.015 ± 0.038	2.107SerArg: 2.107 ± 0.04	4.251SerSer: 4.251 ± 0.065	3.307SerThr: 3.307 ± 0.05	3.883SerVal: 3.883 ± 0.046	0.693SerTrp: 0.693 ± 0.021	2.508SerTyr: 2.508 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	3.53ThrAla: 3.53 ± 0.054	0.361ThrCys: 0.361 ± 0.019	2.702ThrAsp: 2.702 ± 0.046	3.539ThrGlu: 3.539 ± 0.061	2.717ThrPhe: 2.717 ± 0.048	3.801ThrGly: 3.801 ± 0.058	1.024ThrHis: 1.024 ± 0.03	5.064ThrIle: 5.064 ± 0.055	3.733ThrLys: 3.733 ± 0.052	5.269ThrLeu: 5.269 ± 0.054	1.247ThrMet: 1.247 ± 0.028	2.898ThrAsn: 2.898 ± 0.042	2.126ThrPro: 2.126 ± 0.04	1.355ThrGln: 1.355 ± 0.031	1.754ThrArg: 1.754 ± 0.035	3.503ThrSer: 3.503 ± 0.051	3.003ThrThr: 3.003 ± 0.045	4.078ThrVal: 4.078 ± 0.062	0.519ThrTrp: 0.519 ± 0.021	2.192ThrTyr: 2.192 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	4.467ValAla: 4.467 ± 0.068	0.509ValCys: 0.509 ± 0.021	3.464ValAsp: 3.464 ± 0.05	4.895ValGlu: 4.895 ± 0.056	3.005ValPhe: 3.005 ± 0.044	4.187ValGly: 4.187 ± 0.065	1.257ValHis: 1.257 ± 0.028	5.691ValIle: 5.691 ± 0.067	4.864ValLys: 4.864 ± 0.059	6.308ValLeu: 6.308 ± 0.062	1.68ValMet: 1.68 ± 0.039	3.294ValAsn: 3.294 ± 0.048	2.398ValPro: 2.398 ± 0.043	2.171ValGln: 2.171 ± 0.042	2.339ValArg: 2.339 ± 0.039	4.45ValSer: 4.45 ± 0.059	3.983ValThr: 3.983 ± 0.059	4.535ValVal: 4.535 ± 0.073	0.632ValTrp: 0.632 ± 0.022	2.252ValTyr: 2.252 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.568TrpAla: 0.568 ± 0.019	0.092TrpCys: 0.092 ± 0.008	0.6TrpAsp: 0.6 ± 0.024	0.766TrpGlu: 0.766 ± 0.024	0.549TrpPhe: 0.549 ± 0.022	0.721TrpGly: 0.721 ± 0.027	0.234TrpHis: 0.234 ± 0.012	0.9TrpIle: 0.9 ± 0.026	0.767TrpLys: 0.767 ± 0.023	1.159TrpLeu: 1.159 ± 0.028	0.324TrpMet: 0.324 ± 0.015	0.611TrpAsn: 0.611 ± 0.023	0.232TrpPro: 0.232 ± 0.014	0.376TrpGln: 0.376 ± 0.017	0.42TrpArg: 0.42 ± 0.018	0.66TrpSer: 0.66 ± 0.021	0.5TrpThr: 0.5 ± 0.019	0.662TrpVal: 0.662 ± 0.021	0.153TrpTrp: 0.153 ± 0.011	0.397TrpTyr: 0.397 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.029TyrAla: 2.029 ± 0.039	0.348TyrCys: 0.348 ± 0.017	2.094TyrAsp: 2.094 ± 0.037	2.788TyrGlu: 2.788 ± 0.043	2.048TyrPhe: 2.048 ± 0.039	2.47TyrGly: 2.47 ± 0.043	0.968TyrHis: 0.968 ± 0.027	2.794TyrIle: 2.794 ± 0.046	2.524TyrLys: 2.524 ± 0.043	3.868TyrLeu: 3.868 ± 0.053	0.866TyrMet: 0.866 ± 0.025	1.666TyrAsn: 1.666 ± 0.034	1.445TyrPro: 1.445 ± 0.03	1.672TyrGln: 1.672 ± 0.039	1.611TyrArg: 1.611 ± 0.034	2.391TyrSer: 2.391 ± 0.039	1.912TyrThr: 1.912 ± 0.04	2.322TyrVal: 2.322 ± 0.042	0.455TyrTrp: 0.455 ± 0.02	1.694TyrTyr: 1.694 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5220 proteins (1467317 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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