Enterobacteriaceae bacterium ENNIH1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; unclassified Enterobacteriaceae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5131 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I8QA48|A0A2I8QA48_9ENTR 2-iminoacetate synthase ThiH OS=Enterobacteriaceae bacterium ENNIH1 OX=2066051 GN=C2U51_20365 PE=4 SV=1
MM1 pKa = 7.64SDD3 pKa = 4.45DD4 pKa = 3.53VALPLQFTDD13 pKa = 3.5AAASKK18 pKa = 10.04VKK20 pKa = 10.69NLIADD25 pKa = 3.69EE26 pKa = 4.96DD27 pKa = 4.07NPNLKK32 pKa = 10.27LRR34 pKa = 11.84VYY36 pKa = 9.05ITGGGCSGFQYY47 pKa = 11.03GFTFDD52 pKa = 4.96DD53 pKa = 4.21QINDD57 pKa = 3.16GDD59 pKa = 4.0MTIEE63 pKa = 3.95KK64 pKa = 10.11QGVGLVVDD72 pKa = 4.57PMSLQYY78 pKa = 11.16LVGGSVDD85 pKa = 3.52YY86 pKa = 11.39TEE88 pKa = 4.94GLEE91 pKa = 4.08GSRR94 pKa = 11.84FVVTNPNATSTCGCGSSFSII114 pKa = 5.02

Molecular weight: 12.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I8QB35|A0A2I8QB35_9ENTR Uncharacterized protein OS=Enterobacteriaceae bacterium ENNIH1 OX=2066051 GN=C2U51_21910 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5131

0

5131

1564357

15

1862

304.9

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.875 ± 0.041

1.112 ± 0.014

5.211 ± 0.027

5.672 ± 0.033

3.902 ± 0.024

7.364 ± 0.03

2.299 ± 0.017

5.767 ± 0.027

4.233 ± 0.034

10.807 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.758 ± 0.015

3.688 ± 0.021

4.451 ± 0.022

4.388 ± 0.026

5.773 ± 0.031

5.953 ± 0.029

5.422 ± 0.022

7.087 ± 0.026

1.508 ± 0.016

2.731 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski