Moraxella osloensis
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2336 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A378QAS6|A0A378QAS6_MOROS dTDP-glucose 4 6-dehydratase OS=Moraxella osloensis OX=34062 GN=rfbB PE=3 SV=1
MM1 pKa = 7.83 SDD3 pKa = 3.16 TVTDD7 pKa = 3.69 SMVDD11 pKa = 3.52 GVTLTDD17 pKa = 3.33 NAAKK21 pKa = 10.27 KK22 pKa = 9.95 VRR24 pKa = 11.84 NLRR27 pKa = 11.84 DD28 pKa = 3.42 SEE30 pKa = 4.43 GNNDD34 pKa = 3.23 LMLRR38 pKa = 11.84 VYY40 pKa = 9.11 VTGGGCSGFSYY51 pKa = 10.54 GFNFEE56 pKa = 4.7 EE57 pKa = 5.09 NLGDD61 pKa = 5.1 DD62 pKa = 4.73 DD63 pKa = 4.66 ATFSNDD69 pKa = 2.94 DD70 pKa = 3.18 VALVVDD76 pKa = 4.19 SLSYY80 pKa = 10.65 QYY82 pKa = 11.78 LHH84 pKa = 7.14 GSTIDD89 pKa = 3.55 YY90 pKa = 11.2 VEE92 pKa = 4.21 GLEE95 pKa = 4.38 GSRR98 pKa = 11.84 FTVNNPNATTTCGCGSSFSII118 pKa = 5.02
Molecular weight: 12.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.846
IPC_protein 3.821
Toseland 3.592
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.668
EMBOSS 3.795
Sillero 3.948
Patrickios 0.896
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.827
Protein with the highest isoelectric point:
>tr|A0A0X8K713|A0A0X8K713_MOROS Homoserine kinase OS=Moraxella osloensis OX=34062 GN=thrB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVIKK11 pKa = 10.52 RR12 pKa = 11.84 KK13 pKa = 8.26 RR14 pKa = 11.84 THH16 pKa = 5.92 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.03 GRR39 pKa = 11.84 HH40 pKa = 5.34 RR41 pKa = 11.84 LTVV44 pKa = 3.07
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2336
0
2336
750899
29
2904
321.4
35.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.071 ± 0.057
0.886 ± 0.015
5.732 ± 0.041
5.006 ± 0.048
3.949 ± 0.038
6.4 ± 0.062
2.343 ± 0.028
6.614 ± 0.042
5.626 ± 0.034
10.134 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.617 ± 0.027
4.855 ± 0.048
4.15 ± 0.032
5.39 ± 0.062
4.209 ± 0.038
6.134 ± 0.034
6.014 ± 0.046
6.663 ± 0.042
1.156 ± 0.018
3.052 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here