Micromonospora sp. HM5-17

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; unclassified Micromonospora

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N2PJC7|A0A3N2PJC7_9ACTN TetR/AcrR family transcriptional regulator OS=Micromonospora sp. HM5-17 OX=2487710 GN=EF879_04450 PE=4 SV=1
MM1 pKa = 7.39GLIIGAVVLVLLCACGIGGVVWAINQSDD29 pKa = 4.0DD30 pKa = 4.02GDD32 pKa = 4.12PEE34 pKa = 4.28AAPTTSTSASPSAEE48 pKa = 3.82PSPSEE53 pKa = 5.07DD54 pKa = 3.27SGSDD58 pKa = 3.28SGSDD62 pKa = 3.67DD63 pKa = 3.7SGSDD67 pKa = 3.08EE68 pKa = 4.36TDD70 pKa = 3.59FKK72 pKa = 11.6EE73 pKa = 4.93GDD75 pKa = 3.84CLVNDD80 pKa = 4.64GTDD83 pKa = 3.75DD84 pKa = 4.09DD85 pKa = 4.57PEE87 pKa = 4.23LRR89 pKa = 11.84KK90 pKa = 10.15VPCGPNTYY98 pKa = 9.47EE99 pKa = 3.92VLSRR103 pKa = 11.84IPFTTDD109 pKa = 2.52GDD111 pKa = 3.96RR112 pKa = 11.84CIEE115 pKa = 4.17DD116 pKa = 5.16PIFGDD121 pKa = 3.55PDD123 pKa = 3.54TDD125 pKa = 3.45VNYY128 pKa = 10.28VYY130 pKa = 10.5DD131 pKa = 4.42DD132 pKa = 3.91SMDD135 pKa = 3.18IGDD138 pKa = 5.62FVLCLKK144 pKa = 10.53KK145 pKa = 10.57RR146 pKa = 3.55

Molecular weight: 15.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N2PFN3|A0A3N2PFN3_9ACTN Uncharacterized protein OS=Micromonospora sp. HM5-17 OX=2487710 GN=EF879_00365 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84VRR4 pKa = 11.84RR5 pKa = 11.84SLRR8 pKa = 11.84VLKK11 pKa = 10.12RR12 pKa = 11.84KK13 pKa = 9.3PGSVVVRR20 pKa = 11.84RR21 pKa = 11.84HH22 pKa = 4.64GRR24 pKa = 11.84VLVVNRR30 pKa = 11.84TNPRR34 pKa = 11.84WKK36 pKa = 9.18TRR38 pKa = 11.84QGG40 pKa = 3.12

Molecular weight: 4.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5101

0

5101

1718483

27

8061

336.9

36.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.829 ± 0.049

0.74 ± 0.012

5.859 ± 0.029

5.273 ± 0.029

2.554 ± 0.019

9.407 ± 0.035

1.997 ± 0.017

3.353 ± 0.023

1.487 ± 0.021

10.473 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.517 ± 0.014

1.758 ± 0.021

6.665 ± 0.035

2.618 ± 0.02

9.016 ± 0.045

4.623 ± 0.025

6.095 ± 0.032

9.027 ± 0.029

1.566 ± 0.013

2.143 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski