Clostridium sp. CAG:349

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1346 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6WPK9|R6WPK9_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:349 OX=1262797 GN=BN619_01014 PE=4 SV=1
MM1 pKa = 7.64INLNEE6 pKa = 4.01IAFIDD11 pKa = 3.58EE12 pKa = 5.13DD13 pKa = 3.79GFEE16 pKa = 4.62FNTGEE21 pKa = 3.94SLIRR25 pKa = 11.84FDD27 pKa = 4.33VVAYY31 pKa = 10.67CPDD34 pKa = 3.39TKK36 pKa = 10.8QITFAITNQGKK47 pKa = 9.94ISVLDD52 pKa = 3.59FEE54 pKa = 4.95VFEE57 pKa = 5.33DD58 pKa = 4.13EE59 pKa = 3.95NGHH62 pKa = 5.41YY63 pKa = 9.69IEE65 pKa = 5.26YY66 pKa = 10.37GSTYY70 pKa = 9.87EE71 pKa = 5.22KK72 pKa = 10.32IYY74 pKa = 10.48IDD76 pKa = 5.33DD77 pKa = 3.76VQEE80 pKa = 4.32DD81 pKa = 4.41FNN83 pKa = 4.71

Molecular weight: 9.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6X1M4|R6X1M4_9CLOT Phenylacetate-coenzyme A ligase OS=Clostridium sp. CAG:349 OX=1262797 GN=BN619_00603 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.82KK9 pKa = 7.91RR10 pKa = 11.84HH11 pKa = 3.93VTKK14 pKa = 10.46VHH16 pKa = 6.01GFRR19 pKa = 11.84KK20 pKa = 10.08RR21 pKa = 11.84MQTKK25 pKa = 9.63QGRR28 pKa = 11.84NVLKK32 pKa = 10.42RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NKK37 pKa = 9.66GRR39 pKa = 11.84AKK41 pKa = 9.57LTPHH45 pKa = 6.24NN46 pKa = 4.29

Molecular weight: 5.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1346

0

1346

421787

33

2422

313.4

34.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.872 ± 0.065

1.415 ± 0.031

6.281 ± 0.045

6.692 ± 0.059

4.717 ± 0.065

6.948 ± 0.059

1.409 ± 0.027

7.25 ± 0.048

8.253 ± 0.049

8.397 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.038

4.945 ± 0.044

3.166 ± 0.038

2.181 ± 0.035

4.199 ± 0.059

5.719 ± 0.051

5.461 ± 0.058

8.051 ± 0.064

0.649 ± 0.02

3.902 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski