Mycobacterium phage Tonenili

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Bixzunavirus; unclassified Bixzunavirus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 258 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C9EH55|A0A1C9EH55_9CAUD Uncharacterized protein OS=Mycobacterium phage Tonenili OX=1891703 GN=SEA_TONENILI_78 PE=4 SV=1
MM1 pKa = 7.82TDD3 pKa = 3.92FIAYY7 pKa = 9.86SEE9 pKa = 4.57EE10 pKa = 3.63PLGYY14 pKa = 10.29DD15 pKa = 5.17DD16 pKa = 4.36IVAYY20 pKa = 10.4QSFDD24 pKa = 3.57PPTDD28 pKa = 3.44QIPVVSEE35 pKa = 4.16AMVDD39 pKa = 3.49AYY41 pKa = 11.09LALPWWRR48 pKa = 11.84RR49 pKa = 11.84LAVDD53 pKa = 4.6FGLVDD58 pKa = 4.19PLTLVTT64 pKa = 5.18

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C9EHH3|A0A1C9EHH3_9CAUD Aminotransferase OS=Mycobacterium phage Tonenili OX=1891703 GN=SEA_TONENILI_219 PE=4 SV=1
MM1 pKa = 8.07RR2 pKa = 11.84SPKK5 pKa = 10.58VIGDD9 pKa = 3.5PARR12 pKa = 11.84PYY14 pKa = 9.17MLRR17 pKa = 11.84WHH19 pKa = 5.96ILKK22 pKa = 9.21WRR24 pKa = 11.84NLPRR28 pKa = 11.84IYY30 pKa = 10.29VHH32 pKa = 6.91KK33 pKa = 10.21FLRR36 pKa = 11.84SDD38 pKa = 4.15DD39 pKa = 3.81DD40 pKa = 3.92RR41 pKa = 11.84APHH44 pKa = 5.47NHH46 pKa = 6.31PWWFISILLWGKK58 pKa = 8.97YY59 pKa = 7.56VEE61 pKa = 4.7HH62 pKa = 6.87RR63 pKa = 11.84WIDD66 pKa = 3.54GVQQTRR72 pKa = 11.84LRR74 pKa = 11.84RR75 pKa = 11.84APSIAFRR82 pKa = 11.84PISTRR87 pKa = 11.84HH88 pKa = 4.77RR89 pKa = 11.84VEE91 pKa = 5.31LVEE94 pKa = 3.93GWYY97 pKa = 10.4DD98 pKa = 3.52FNSRR102 pKa = 11.84YY103 pKa = 10.04GIRR106 pKa = 11.84PAWTLIITGRR116 pKa = 11.84DD117 pKa = 3.25VRR119 pKa = 11.84GWGFWCPHH127 pKa = 4.69EE128 pKa = 4.81RR129 pKa = 11.84FVPSHH134 pKa = 6.02EE135 pKa = 3.94FRR137 pKa = 11.84GCGEE141 pKa = 3.85

Molecular weight: 17.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

258

0

258

49306

20

1267

191.1

21.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.786 ± 0.208

0.892 ± 0.082

7.052 ± 0.166

6.445 ± 0.169

3.077 ± 0.099

7.849 ± 0.251

2.466 ± 0.145

4.365 ± 0.127

3.764 ± 0.127

7.638 ± 0.194

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.539 ± 0.101

3.294 ± 0.12

5.679 ± 0.145

4.01 ± 0.131

6.916 ± 0.179

5.523 ± 0.145

6.407 ± 0.186

7.303 ± 0.156

1.911 ± 0.081

3.085 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski