Amino acid dipepetide frequency for Pseudomonas phage LKA5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.479AlaAla: 16.479 ± 1.465
0.841AlaCys: 0.841 ± 0.322
8.66AlaAsp: 8.66 ± 0.821
8.759AlaGlu: 8.759 ± 0.895
3.118AlaPhe: 3.118 ± 0.316
10.144AlaGly: 10.144 ± 0.869
1.979AlaHis: 1.979 ± 0.372
5.542AlaIle: 5.542 ± 0.601
6.235AlaLys: 6.235 ± 0.894
10.144AlaLeu: 10.144 ± 1.079
3.365AlaMet: 3.365 ± 0.447
3.019AlaAsn: 3.019 ± 0.424
6.631AlaPro: 6.631 ± 0.734
6.928AlaGln: 6.928 ± 0.853
8.61AlaArg: 8.61 ± 0.689
6.037AlaSer: 6.037 ± 0.666
5.245AlaThr: 5.245 ± 0.697
8.215AlaVal: 8.215 ± 0.728
2.029AlaTrp: 2.029 ± 0.342
3.068AlaTyr: 3.068 ± 0.499
0.0AlaXaa: 0.0 ± 0.0
Cys
0.693CysAla: 0.693 ± 0.234
0.346CysCys: 0.346 ± 0.156
0.643CysAsp: 0.643 ± 0.264
0.544CysGlu: 0.544 ± 0.203
0.099CysPhe: 0.099 ± 0.082
1.039CysGly: 1.039 ± 0.305
0.099CysHis: 0.099 ± 0.08
0.396CysIle: 0.396 ± 0.138
0.396CysLys: 0.396 ± 0.141
0.841CysLeu: 0.841 ± 0.255
0.099CysMet: 0.099 ± 0.082
0.297CysAsn: 0.297 ± 0.151
0.396CysPro: 0.396 ± 0.159
0.445CysGln: 0.445 ± 0.19
0.594CysArg: 0.594 ± 0.243
0.693CysSer: 0.693 ± 0.261
0.297CysThr: 0.297 ± 0.155
0.495CysVal: 0.495 ± 0.222
0.099CysTrp: 0.099 ± 0.069
0.297CysTyr: 0.297 ± 0.148
0.0CysXaa: 0.0 ± 0.0
Asp
9.056AspAla: 9.056 ± 0.83
0.445AspCys: 0.445 ± 0.195
3.513AspAsp: 3.513 ± 0.436
3.761AspGlu: 3.761 ± 0.535
1.831AspPhe: 1.831 ± 0.324
5.196AspGly: 5.196 ± 0.612
0.94AspHis: 0.94 ± 0.206
2.87AspIle: 2.87 ± 0.304
1.831AspLys: 1.831 ± 0.333
4.949AspLeu: 4.949 ± 0.447
2.177AspMet: 2.177 ± 0.304
1.435AspAsn: 1.435 ± 0.281
3.464AspPro: 3.464 ± 0.574
3.118AspGln: 3.118 ± 0.416
3.959AspArg: 3.959 ± 0.535
3.217AspSer: 3.217 ± 0.397
3.414AspThr: 3.414 ± 0.422
4.107AspVal: 4.107 ± 0.502
1.039AspTrp: 1.039 ± 0.241
1.683AspTyr: 1.683 ± 0.358
0.0AspXaa: 0.0 ± 0.0
Glu
8.363GluAla: 8.363 ± 1.008
0.544GluCys: 0.544 ± 0.18
2.87GluAsp: 2.87 ± 0.461
3.217GluGlu: 3.217 ± 0.511
2.078GluPhe: 2.078 ± 0.513
4.652GluGly: 4.652 ± 0.586
1.138GluHis: 1.138 ± 0.235
2.821GluIle: 2.821 ± 0.584
2.771GluLys: 2.771 ± 0.466
6.136GluLeu: 6.136 ± 0.609
1.188GluMet: 1.188 ± 0.223
1.633GluAsn: 1.633 ± 0.235
2.672GluPro: 2.672 ± 0.464
4.503GluGln: 4.503 ± 0.522
5.295GluArg: 5.295 ± 0.607
3.761GluSer: 3.761 ± 0.548
2.524GluThr: 2.524 ± 0.403
3.711GluVal: 3.711 ± 0.525
0.99GluTrp: 0.99 ± 0.288
1.88GluTyr: 1.88 ± 0.323
0.0GluXaa: 0.0 ± 0.0
Phe
3.118PheAla: 3.118 ± 0.394
0.198PheCys: 0.198 ± 0.098
1.93PheAsp: 1.93 ± 0.372
2.029PheGlu: 2.029 ± 0.4
0.495PhePhe: 0.495 ± 0.131
2.722PheGly: 2.722 ± 0.515
0.594PheHis: 0.594 ± 0.191
1.039PheIle: 1.039 ± 0.266
1.237PheLys: 1.237 ± 0.262
2.078PheLeu: 2.078 ± 0.326
0.445PheMet: 0.445 ± 0.136
0.94PheAsn: 0.94 ± 0.228
1.633PhePro: 1.633 ± 0.33
1.237PheGln: 1.237 ± 0.182
2.029PheArg: 2.029 ± 0.332
1.732PheSer: 1.732 ± 0.246
1.485PheThr: 1.485 ± 0.336
2.177PheVal: 2.177 ± 0.458
0.445PheTrp: 0.445 ± 0.233
0.99PheTyr: 0.99 ± 0.33
0.0PheXaa: 0.0 ± 0.0
Gly
8.165GlyAla: 8.165 ± 0.819
0.94GlyCys: 0.94 ± 0.307
4.602GlyAsp: 4.602 ± 0.461
5.196GlyGlu: 5.196 ± 0.721
3.019GlyPhe: 3.019 ± 0.535
6.334GlyGly: 6.334 ± 0.7
1.188GlyHis: 1.188 ± 0.211
3.909GlyIle: 3.909 ± 0.508
3.266GlyLys: 3.266 ± 0.465
6.235GlyLeu: 6.235 ± 0.511
1.979GlyMet: 1.979 ± 0.341
2.326GlyAsn: 2.326 ± 0.323
2.771GlyPro: 2.771 ± 0.46
4.998GlyGln: 4.998 ± 0.537
6.037GlyArg: 6.037 ± 0.604
4.503GlySer: 4.503 ± 0.537
5.097GlyThr: 5.097 ± 0.773
5.493GlyVal: 5.493 ± 0.523
1.435GlyTrp: 1.435 ± 0.261
2.524GlyTyr: 2.524 ± 0.378
0.0GlyXaa: 0.0 ± 0.0
His
2.029HisAla: 2.029 ± 0.246
0.198HisCys: 0.198 ± 0.116
1.138HisAsp: 1.138 ± 0.302
1.287HisGlu: 1.287 ± 0.254
0.841HisPhe: 0.841 ± 0.249
1.88HisGly: 1.88 ± 0.268
0.594HisHis: 0.594 ± 0.199
0.643HisIle: 0.643 ± 0.227
0.346HisLys: 0.346 ± 0.124
1.683HisLeu: 1.683 ± 0.504
0.544HisMet: 0.544 ± 0.164
0.693HisAsn: 0.693 ± 0.221
0.891HisPro: 0.891 ± 0.236
0.891HisGln: 0.891 ± 0.172
2.029HisArg: 2.029 ± 0.278
1.039HisSer: 1.039 ± 0.365
1.089HisThr: 1.089 ± 0.28
1.287HisVal: 1.287 ± 0.221
0.297HisTrp: 0.297 ± 0.117
0.594HisTyr: 0.594 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
5.146IleAla: 5.146 ± 0.453
0.643IleCys: 0.643 ± 0.221
3.316IleAsp: 3.316 ± 0.305
3.316IleGlu: 3.316 ± 0.455
0.594IlePhe: 0.594 ± 0.178
3.316IleGly: 3.316 ± 0.41
0.99IleHis: 0.99 ± 0.273
1.039IleIle: 1.039 ± 0.267
2.078IleLys: 2.078 ± 0.349
2.474IleLeu: 2.474 ± 0.3
0.594IleMet: 0.594 ± 0.142
1.831IleAsn: 1.831 ± 0.255
2.375IlePro: 2.375 ± 0.301
1.138IleGln: 1.138 ± 0.222
2.821IleArg: 2.821 ± 0.323
2.078IleSer: 2.078 ± 0.41
2.771IleThr: 2.771 ± 0.408
2.425IleVal: 2.425 ± 0.403
0.891IleTrp: 0.891 ± 0.259
1.188IleTyr: 1.188 ± 0.354
0.0IleXaa: 0.0 ± 0.0
Lys
4.949LysAla: 4.949 ± 0.808
0.346LysCys: 0.346 ± 0.153
2.573LysAsp: 2.573 ± 0.396
2.326LysGlu: 2.326 ± 0.4
1.039LysPhe: 1.039 ± 0.213
2.969LysGly: 2.969 ± 0.417
0.841LysHis: 0.841 ± 0.232
0.99LysIle: 0.99 ± 0.254
1.93LysLys: 1.93 ± 0.35
3.464LysLeu: 3.464 ± 0.385
1.089LysMet: 1.089 ± 0.226
0.643LysAsn: 0.643 ± 0.173
2.524LysPro: 2.524 ± 0.303
1.979LysGln: 1.979 ± 0.353
2.573LysArg: 2.573 ± 0.388
2.029LysSer: 2.029 ± 0.407
2.177LysThr: 2.177 ± 0.393
2.672LysVal: 2.672 ± 0.356
0.396LysTrp: 0.396 ± 0.151
0.99LysTyr: 0.99 ± 0.212
0.0LysXaa: 0.0 ± 0.0
Leu
11.431LeuAla: 11.431 ± 0.869
0.544LeuCys: 0.544 ± 0.203
5.146LeuAsp: 5.146 ± 0.467
4.553LeuGlu: 4.553 ± 0.393
2.375LeuPhe: 2.375 ± 0.339
5.493LeuGly: 5.493 ± 0.592
1.88LeuHis: 1.88 ± 0.366
2.672LeuIle: 2.672 ± 0.466
2.821LeuLys: 2.821 ± 0.295
6.779LeuLeu: 6.779 ± 0.804
2.029LeuMet: 2.029 ± 0.288
3.266LeuAsn: 3.266 ± 0.41
4.602LeuPro: 4.602 ± 0.661
3.414LeuGln: 3.414 ± 0.335
6.681LeuArg: 6.681 ± 0.627
4.85LeuSer: 4.85 ± 0.487
4.949LeuThr: 4.949 ± 0.547
6.037LeuVal: 6.037 ± 0.686
0.99LeuTrp: 0.99 ± 0.222
1.633LeuTyr: 1.633 ± 0.351
0.0LeuXaa: 0.0 ± 0.0
Met
2.969MetAla: 2.969 ± 0.529
0.049MetCys: 0.049 ± 0.063
1.039MetAsp: 1.039 ± 0.237
0.742MetGlu: 0.742 ± 0.233
0.396MetPhe: 0.396 ± 0.17
2.177MetGly: 2.177 ± 0.464
0.445MetHis: 0.445 ± 0.16
1.039MetIle: 1.039 ± 0.194
1.039MetLys: 1.039 ± 0.215
2.227MetLeu: 2.227 ± 0.263
0.445MetMet: 0.445 ± 0.153
0.742MetAsn: 0.742 ± 0.177
2.227MetPro: 2.227 ± 0.292
1.237MetGln: 1.237 ± 0.303
1.584MetArg: 1.584 ± 0.46
1.831MetSer: 1.831 ± 0.324
1.88MetThr: 1.88 ± 0.261
1.039MetVal: 1.039 ± 0.294
0.247MetTrp: 0.247 ± 0.119
0.594MetTyr: 0.594 ± 0.141
0.0MetXaa: 0.0 ± 0.0
Asn
3.662AsnAla: 3.662 ± 0.462
0.247AsnCys: 0.247 ± 0.124
1.633AsnAsp: 1.633 ± 0.342
1.386AsnGlu: 1.386 ± 0.247
0.792AsnPhe: 0.792 ± 0.201
2.524AsnGly: 2.524 ± 0.363
0.445AsnHis: 0.445 ± 0.146
1.237AsnIle: 1.237 ± 0.342
1.089AsnLys: 1.089 ± 0.22
2.672AsnLeu: 2.672 ± 0.397
1.089AsnMet: 1.089 ± 0.245
0.742AsnAsn: 0.742 ± 0.238
2.227AsnPro: 2.227 ± 0.354
1.633AsnGln: 1.633 ± 0.341
2.177AsnArg: 2.177 ± 0.313
1.93AsnSer: 1.93 ± 0.287
1.683AsnThr: 1.683 ± 0.216
1.534AsnVal: 1.534 ± 0.285
0.643AsnTrp: 0.643 ± 0.232
0.693AsnTyr: 0.693 ± 0.237
0.0AsnXaa: 0.0 ± 0.0
Pro
7.769ProAla: 7.769 ± 0.549
0.396ProCys: 0.396 ± 0.18
4.454ProAsp: 4.454 ± 0.548
4.206ProGlu: 4.206 ± 0.378
1.386ProPhe: 1.386 ± 0.323
3.711ProGly: 3.711 ± 0.651
1.089ProHis: 1.089 ± 0.318
2.227ProIle: 2.227 ± 0.557
1.732ProLys: 1.732 ± 0.321
3.464ProLeu: 3.464 ± 0.463
1.485ProMet: 1.485 ± 0.263
1.781ProAsn: 1.781 ± 0.38
3.909ProPro: 3.909 ± 1.085
2.326ProGln: 2.326 ± 0.441
3.118ProArg: 3.118 ± 0.426
3.167ProSer: 3.167 ± 0.361
3.266ProThr: 3.266 ± 0.307
3.86ProVal: 3.86 ± 0.5
0.693ProTrp: 0.693 ± 0.16
1.633ProTyr: 1.633 ± 0.35
0.0ProXaa: 0.0 ± 0.0
Gln
8.363GlnAla: 8.363 ± 0.724
0.346GlnCys: 0.346 ± 0.151
2.227GlnAsp: 2.227 ± 0.351
3.068GlnGlu: 3.068 ± 0.363
1.633GlnPhe: 1.633 ± 0.273
3.86GlnGly: 3.86 ± 0.53
1.534GlnHis: 1.534 ± 0.253
2.87GlnIle: 2.87 ± 0.627
1.089GlnLys: 1.089 ± 0.244
3.019GlnLeu: 3.019 ± 0.396
1.237GlnMet: 1.237 ± 0.307
1.237GlnAsn: 1.237 ± 0.38
2.87GlnPro: 2.87 ± 0.302
4.305GlnGln: 4.305 ± 0.683
3.266GlnArg: 3.266 ± 0.523
2.375GlnSer: 2.375 ± 0.407
1.633GlnThr: 1.633 ± 0.251
3.464GlnVal: 3.464 ± 0.398
0.841GlnTrp: 0.841 ± 0.259
1.88GlnTyr: 1.88 ± 0.311
0.0GlnXaa: 0.0 ± 0.0
Arg
7.868ArgAla: 7.868 ± 0.742
0.742ArgCys: 0.742 ± 0.234
4.206ArgAsp: 4.206 ± 0.628
4.998ArgGlu: 4.998 ± 0.759
2.969ArgPhe: 2.969 ± 0.375
5.245ArgGly: 5.245 ± 0.747
1.386ArgHis: 1.386 ± 0.314
3.563ArgIle: 3.563 ± 0.439
3.118ArgLys: 3.118 ± 0.393
7.027ArgLeu: 7.027 ± 0.592
1.435ArgMet: 1.435 ± 0.232
2.276ArgAsn: 2.276 ± 0.326
3.662ArgPro: 3.662 ± 0.522
4.058ArgGln: 4.058 ± 0.541
6.136ArgArg: 6.136 ± 0.653
3.563ArgSer: 3.563 ± 0.705
3.563ArgThr: 3.563 ± 0.401
5.344ArgVal: 5.344 ± 0.485
1.287ArgTrp: 1.287 ± 0.32
1.979ArgTyr: 1.979 ± 0.43
0.0ArgXaa: 0.0 ± 0.0
Ser
6.037SerAla: 6.037 ± 0.754
0.346SerCys: 0.346 ± 0.177
3.662SerAsp: 3.662 ± 0.541
3.662SerGlu: 3.662 ± 0.498
1.386SerPhe: 1.386 ± 0.236
4.998SerGly: 4.998 ± 0.651
1.089SerHis: 1.089 ± 0.313
1.979SerIle: 1.979 ± 0.342
2.078SerLys: 2.078 ± 0.291
4.998SerLeu: 4.998 ± 0.479
1.534SerMet: 1.534 ± 0.254
1.93SerAsn: 1.93 ± 0.296
3.365SerPro: 3.365 ± 0.528
2.276SerGln: 2.276 ± 0.386
3.365SerArg: 3.365 ± 0.337
2.969SerSer: 2.969 ± 0.358
3.167SerThr: 3.167 ± 0.559
3.464SerVal: 3.464 ± 0.325
0.891SerTrp: 0.891 ± 0.205
1.485SerTyr: 1.485 ± 0.365
0.0SerXaa: 0.0 ± 0.0
Thr
6.779ThrAla: 6.779 ± 0.944
0.396ThrCys: 0.396 ± 0.13
3.266ThrAsp: 3.266 ± 0.346
2.524ThrGlu: 2.524 ± 0.389
1.93ThrPhe: 1.93 ± 0.385
5.394ThrGly: 5.394 ± 0.568
0.841ThrHis: 0.841 ± 0.202
2.227ThrIle: 2.227 ± 0.401
1.633ThrLys: 1.633 ± 0.284
4.008ThrLeu: 4.008 ± 0.466
0.792ThrMet: 0.792 ± 0.207
1.633ThrAsn: 1.633 ± 0.285
3.711ThrPro: 3.711 ± 0.416
1.781ThrGln: 1.781 ± 0.342
3.86ThrArg: 3.86 ± 0.487
3.068ThrSer: 3.068 ± 0.356
2.969ThrThr: 2.969 ± 0.633
4.008ThrVal: 4.008 ± 0.57
0.742ThrTrp: 0.742 ± 0.195
1.435ThrTyr: 1.435 ± 0.214
0.0ThrXaa: 0.0 ± 0.0
Val
7.967ValAla: 7.967 ± 0.66
0.594ValCys: 0.594 ± 0.205
4.701ValAsp: 4.701 ± 0.524
5.097ValGlu: 5.097 ± 0.808
1.435ValPhe: 1.435 ± 0.376
4.949ValGly: 4.949 ± 0.486
1.831ValHis: 1.831 ± 0.354
2.87ValIle: 2.87 ± 0.508
2.425ValLys: 2.425 ± 0.382
5.79ValLeu: 5.79 ± 0.558
1.584ValMet: 1.584 ± 0.235
1.633ValAsn: 1.633 ± 0.224
3.761ValPro: 3.761 ± 0.602
3.266ValGln: 3.266 ± 0.564
5.839ValArg: 5.839 ± 0.626
3.563ValSer: 3.563 ± 0.436
3.316ValThr: 3.316 ± 0.391
4.206ValVal: 4.206 ± 0.465
0.643ValTrp: 0.643 ± 0.257
1.138ValTyr: 1.138 ± 0.291
0.0ValXaa: 0.0 ± 0.0
Trp
1.831TrpAla: 1.831 ± 0.308
0.297TrpCys: 0.297 ± 0.158
0.693TrpAsp: 0.693 ± 0.18
0.742TrpGlu: 0.742 ± 0.187
0.198TrpPhe: 0.198 ± 0.115
1.287TrpGly: 1.287 ± 0.326
0.594TrpHis: 0.594 ± 0.142
0.099TrpIle: 0.099 ± 0.085
0.594TrpLys: 0.594 ± 0.159
2.078TrpLeu: 2.078 ± 0.528
0.297TrpMet: 0.297 ± 0.119
0.693TrpAsn: 0.693 ± 0.161
0.594TrpPro: 0.594 ± 0.151
0.445TrpGln: 0.445 ± 0.123
1.485TrpArg: 1.485 ± 0.268
0.594TrpSer: 0.594 ± 0.225
0.891TrpThr: 0.891 ± 0.192
1.188TrpVal: 1.188 ± 0.303
0.198TrpTrp: 0.198 ± 0.078
0.445TrpTyr: 0.445 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.375TyrAla: 2.375 ± 0.425
0.346TyrCys: 0.346 ± 0.128
1.732TyrAsp: 1.732 ± 0.259
1.386TyrGlu: 1.386 ± 0.365
0.94TyrPhe: 0.94 ± 0.256
2.128TyrGly: 2.128 ± 0.403
0.495TyrHis: 0.495 ± 0.159
0.99TyrIle: 0.99 ± 0.176
0.792TyrLys: 0.792 ± 0.227
2.078TyrLeu: 2.078 ± 0.31
0.495TyrMet: 0.495 ± 0.187
1.287TyrAsn: 1.287 ± 0.353
1.386TyrPro: 1.386 ± 0.319
1.138TyrGln: 1.138 ± 0.236
2.969TyrArg: 2.969 ± 0.335
1.683TyrSer: 1.683 ± 0.375
1.584TyrThr: 1.584 ± 0.253
1.88TyrVal: 1.88 ± 0.428
0.445TyrTrp: 0.445 ± 0.199
0.297TyrTyr: 0.297 ± 0.109
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (20209 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski