Coprococcus catus
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3079 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3E2XS14|A0A3E2XS14_9FIRM Stage 0 sporulation protein A homolog OS=Coprococcus catus OX=116085 GN=DW747_01320 PE=4 SV=1
MM1 pKa = 7.68 KK2 pKa = 10.31 KK3 pKa = 10.06 RR4 pKa = 11.84 VITGLMAASVIVSLAGCGAGQKK26 pKa = 10.34 EE27 pKa = 4.91 SVEE30 pKa = 4.28 STAPATTTEE39 pKa = 4.34 TVAASQVEE47 pKa = 4.4 TAEE50 pKa = 3.94 ATEE53 pKa = 4.33 TEE55 pKa = 4.39 KK56 pKa = 10.37 TVQEE60 pKa = 4.03 DD61 pKa = 3.14 RR62 pKa = 11.84 AARR65 pKa = 11.84 IKK67 pKa = 10.49 AYY69 pKa = 10.0 QAALDD74 pKa = 4.4 NIYY77 pKa = 10.48 QNHH80 pKa = 6.76 ILPDD84 pKa = 3.99 GSEE87 pKa = 3.99 LRR89 pKa = 11.84 DD90 pKa = 4.54 DD91 pKa = 4.26 ISGEE95 pKa = 3.69 MSGNKK100 pKa = 9.14 FAVYY104 pKa = 10.25 DD105 pKa = 3.59 VDD107 pKa = 4.21 HH108 pKa = 7.9 DD109 pKa = 4.04 DD110 pKa = 3.99 RR111 pKa = 11.84 EE112 pKa = 4.32 EE113 pKa = 4.52 LVFCYY118 pKa = 8.97 TATSMASMIAKK129 pKa = 9.89 IYY131 pKa = 10.66 DD132 pKa = 3.34 YY133 pKa = 11.45 DD134 pKa = 3.79 EE135 pKa = 5.09 ASGEE139 pKa = 4.12 LYY141 pKa = 10.7 EE142 pKa = 4.86 EE143 pKa = 5.11 LSQFPMLTFYY153 pKa = 11.57 DD154 pKa = 3.78 NATIGAGISHH164 pKa = 6.0 NQGYY168 pKa = 10.48 AGEE171 pKa = 4.42 FWPYY175 pKa = 10.18 TFYY178 pKa = 10.67 TYY180 pKa = 11.16 DD181 pKa = 3.55 AANDD185 pKa = 3.8 SYY187 pKa = 10.8 RR188 pKa = 11.84 AEE190 pKa = 4.28 YY191 pKa = 10.07 FVDD194 pKa = 4.03 AWDD197 pKa = 4.08 KK198 pKa = 11.27 SLSEE202 pKa = 3.96 VNYY205 pKa = 10.55 NGEE208 pKa = 4.44 AFPDD212 pKa = 4.14 DD213 pKa = 4.23 ADD215 pKa = 4.05 KK216 pKa = 11.58 DD217 pKa = 3.89 GDD219 pKa = 4.17 GIVYY223 pKa = 10.27 YY224 pKa = 10.9 LEE226 pKa = 5.49 APDD229 pKa = 4.34 GSEE232 pKa = 3.79 TGEE235 pKa = 4.51 PLDD238 pKa = 5.51 KK239 pKa = 11.04 DD240 pKa = 5.69 AFDD243 pKa = 3.34 QWISEE248 pKa = 4.23 NVGKK252 pKa = 10.6 EE253 pKa = 3.87 EE254 pKa = 4.85 NIPFQDD260 pKa = 3.53 LTEE263 pKa = 4.51 DD264 pKa = 4.78 AISQIQQ270 pKa = 3.04
Molecular weight: 29.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.897
IPC_protein 3.884
Toseland 3.681
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.592
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.177
Thurlkill 3.719
EMBOSS 3.783
Sillero 3.999
Patrickios 1.291
IPC_peptide 3.859
IPC2_peptide 3.986
IPC2.peptide.svr19 3.898
Protein with the highest isoelectric point:
>tr|A0A3E2TNR7|A0A3E2TNR7_9FIRM Stp1/IreP family PP2C-type Ser/Thr phosphatase OS=Coprococcus catus OX=116085 GN=DW070_07630 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.71 MTFQPKK8 pKa = 7.78 TRR10 pKa = 11.84 QRR12 pKa = 11.84 SKK14 pKa = 8.94 VHH16 pKa = 5.99 GFRR19 pKa = 11.84 KK20 pKa = 10.0 RR21 pKa = 11.84 MSTANGRR28 pKa = 11.84 KK29 pKa = 8.89 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.71 GRR39 pKa = 11.84 KK40 pKa = 8.73 VLSAA44 pKa = 4.05
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.647
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.369
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3079
0
3079
982488
27
2929
319.1
35.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.872 ± 0.047
1.522 ± 0.017
5.923 ± 0.035
7.269 ± 0.054
3.892 ± 0.032
7.022 ± 0.039
1.955 ± 0.023
7.38 ± 0.046
6.52 ± 0.042
8.837 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.349 ± 0.027
4.245 ± 0.032
3.272 ± 0.024
3.445 ± 0.027
4.441 ± 0.038
5.734 ± 0.036
5.426 ± 0.038
6.907 ± 0.04
0.893 ± 0.015
4.095 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here