Nocardioides albidus
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4085 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C4WPK9|A0A5C4WPK9_9ACTN ABC transporter permease OS=Nocardioides albidus OX=1517589 GN=FHP29_00800 PE=3 SV=1
MM1 pKa = 7.51 NLRR4 pKa = 11.84 LADD7 pKa = 3.85 AQNQPFASEE16 pKa = 4.25 EE17 pKa = 4.15 ACTSYY22 pKa = 10.91 AAQGGVVQPYY32 pKa = 9.64 KK33 pKa = 10.9 AVVDD37 pKa = 3.93 SDD39 pKa = 3.59 GDD41 pKa = 4.13 TVSDD45 pKa = 4.13 ADD47 pKa = 4.11 EE48 pKa = 4.13 LAIGQRR54 pKa = 11.84 ADD56 pKa = 3.08 VYY58 pKa = 11.37 NGVDD62 pKa = 3.79 CRR64 pKa = 11.84 TATPRR69 pKa = 11.84 IAAGADD75 pKa = 3.19 LSGCVLRR82 pKa = 11.84 SAVLPTFFLWGTDD95 pKa = 3.74 FEE97 pKa = 4.84 NADD100 pKa = 3.91 LTDD103 pKa = 4.38 ADD105 pKa = 4.93 LMGAQLDD112 pKa = 3.81 QSNLSGANLSGADD125 pKa = 3.74 LSFAYY130 pKa = 10.56 VNNANLAGADD140 pKa = 3.44 LTGAHH145 pKa = 7.14 LAHH148 pKa = 6.67 AQLASYY154 pKa = 10.51 LVGADD159 pKa = 3.56 FTSTDD164 pKa = 3.34 RR165 pKa = 11.84 SDD167 pKa = 4.0 VLWITPFGSATCPDD181 pKa = 3.31 GTEE184 pKa = 3.82 AFLNEE189 pKa = 3.79 GGTCEE194 pKa = 4.04 GHH196 pKa = 7.36 LFDD199 pKa = 6.06 IPP201 pKa = 3.55
Molecular weight: 20.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.719
IPC_protein 3.732
Toseland 3.503
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.719
Rodwell 3.554
Grimsley 3.414
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.151
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.859
Patrickios 0.744
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.761
Protein with the highest isoelectric point:
>tr|A0A5C4WCZ4|A0A5C4WCZ4_9ACTN Uncharacterized protein OS=Nocardioides albidus OX=1517589 GN=FHP29_03985 PE=4 SV=1
MM1 pKa = 7.61 PSRR4 pKa = 11.84 RR5 pKa = 11.84 GRR7 pKa = 11.84 SRR9 pKa = 11.84 QPPSTSAHH17 pKa = 6.86 RR18 pKa = 11.84 ARR20 pKa = 11.84 SHH22 pKa = 5.76 PRR24 pKa = 11.84 GGRR27 pKa = 11.84 GPRR30 pKa = 11.84 PAGRR34 pKa = 11.84 RR35 pKa = 11.84 PGRR38 pKa = 11.84 GHH40 pKa = 7.22 ARR42 pKa = 11.84 PPHH45 pKa = 5.11 PTARR49 pKa = 11.84 RR50 pKa = 11.84 PGPFRR55 pKa = 11.84 ARR57 pKa = 11.84 PPHH60 pKa = 7.03 RR61 pKa = 11.84 YY62 pKa = 9.22 RR63 pKa = 11.84 NAARR67 pKa = 11.84 PPRR70 pKa = 11.84 PCARR74 pKa = 11.84 LQPSQGRR81 pKa = 11.84 TATQPQPRR89 pKa = 11.84 AARR92 pKa = 11.84 AAPTRR97 pKa = 11.84 PRR99 pKa = 11.84 WRR101 pKa = 11.84 RR102 pKa = 11.84 TAAGPRR108 pKa = 11.84 TPPPPRR114 pKa = 11.84 SGRR117 pKa = 11.84 AA118 pKa = 3.03
Molecular weight: 13.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.519
IPC2_protein 11.316
IPC_protein 12.822
Toseland 12.983
ProMoST 13.481
Dawson 12.983
Bjellqvist 12.983
Wikipedia 13.466
Rodwell 12.486
Grimsley 13.027
Solomon 13.481
Lehninger 13.378
Nozaki 12.983
DTASelect 12.983
Thurlkill 12.983
EMBOSS 13.481
Sillero 12.983
Patrickios 12.193
IPC_peptide 13.495
IPC2_peptide 12.471
IPC2.peptide.svr19 9.221
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4085
0
4085
1314685
33
3541
321.8
34.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.399 ± 0.047
0.741 ± 0.009
6.554 ± 0.032
5.829 ± 0.04
2.741 ± 0.021
9.287 ± 0.037
2.176 ± 0.019
3.625 ± 0.027
1.988 ± 0.031
10.218 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.785 ± 0.015
1.703 ± 0.02
5.708 ± 0.029
2.7 ± 0.018
7.745 ± 0.043
5.117 ± 0.024
5.935 ± 0.031
9.334 ± 0.034
1.478 ± 0.017
1.937 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here