Vibrio phage VH2_2019

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9SW79|A0A6B9SW79_9CAUD Deoxycytidine triphosphate deaminase OS=Vibrio phage VH2_2019 OX=2686309 GN=dcd PE=4 SV=1
MM1 pKa = 7.64SYY3 pKa = 10.44YY4 pKa = 10.16INSAAEE10 pKa = 3.56AVQRR14 pKa = 11.84FNEE17 pKa = 4.08QLEE20 pKa = 4.31EE21 pKa = 4.41TPASLQDD28 pKa = 3.4GKK30 pKa = 11.02ILVDD34 pKa = 4.12TIQDD38 pKa = 4.05YY39 pKa = 11.57AEE41 pKa = 4.74HH42 pKa = 7.76DD43 pKa = 4.15YY44 pKa = 11.73DD45 pKa = 5.26CLNQEE50 pKa = 3.86WANWLDD56 pKa = 3.47MMVDD60 pKa = 3.98TNQAPAEE67 pKa = 3.9ARR69 pKa = 11.84EE70 pKa = 4.13WDD72 pKa = 3.63WEE74 pKa = 4.21DD75 pKa = 3.94EE76 pKa = 4.22II77 pKa = 6.47

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9SXU8|A0A6B9SXU8_9CAUD Uncharacterized protein OS=Vibrio phage VH2_2019 OX=2686309 GN=VH22019_00080 PE=4 SV=1
MM1 pKa = 7.48AKK3 pKa = 10.23AVTKK7 pKa = 9.98MLLIDD12 pKa = 4.07GVGFTYY18 pKa = 10.5RR19 pKa = 11.84ANVKK23 pKa = 10.19SRR25 pKa = 11.84LQEE28 pKa = 3.34VDD30 pKa = 3.52LTVKK34 pKa = 10.44NSQGRR39 pKa = 11.84VVASANIMRR48 pKa = 11.84HH49 pKa = 3.61NHH51 pKa = 4.25FTYY54 pKa = 10.25TITKK58 pKa = 8.1ARR60 pKa = 11.84KK61 pKa = 8.3VSGSKK66 pKa = 10.41KK67 pKa = 9.52LLEE70 pKa = 4.41VAHH73 pKa = 5.89SRR75 pKa = 11.84KK76 pKa = 9.39PEE78 pKa = 3.77YY79 pKa = 11.32YY80 pKa = 7.04MTQVAMWLAKK90 pKa = 10.17KK91 pKa = 10.37CC92 pKa = 3.71

Molecular weight: 10.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

108

0

108

25001

65

1376

231.5

25.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.932 ± 0.412

1.136 ± 0.107

5.904 ± 0.158

7.104 ± 0.192

3.096 ± 0.12

6.668 ± 0.174

2.148 ± 0.159

5.464 ± 0.149

6.56 ± 0.252

8.672 ± 0.209

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.036 ± 0.126

4.364 ± 0.184

3.916 ± 0.207

3.72 ± 0.14

4.408 ± 0.177

6.156 ± 0.168

7.116 ± 0.228

7.544 ± 0.239

1.256 ± 0.109

3.804 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski