Parainfluenza virus 5 (strain W3) (PIV5) (Simian virus 5)
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q88435|NCAP_PIV5 Nucleoprotein OS=Parainfluenza virus 5 (strain W3) OX=11208 GN=NP PE=1 SV=1
MM1 pKa = 7.56 SSVLKK6 pKa = 10.47 AYY8 pKa = 9.85 EE9 pKa = 4.19 RR10 pKa = 11.84 FTLTQEE16 pKa = 4.31 LQDD19 pKa = 3.52 QSEE22 pKa = 4.75 EE23 pKa = 4.22 GTIPPTTLKK32 pKa = 10.47 PVIRR36 pKa = 11.84 VFILTSNNPEE46 pKa = 3.83 LRR48 pKa = 11.84 SRR50 pKa = 11.84 LLLFCLRR57 pKa = 11.84 IVLSNGARR65 pKa = 11.84 DD66 pKa = 3.57 SHH68 pKa = 7.35 RR69 pKa = 11.84 FGALLTMFSLPSATMLNHH87 pKa = 6.25 VKK89 pKa = 10.41 LADD92 pKa = 3.6 QSPEE96 pKa = 3.53 ADD98 pKa = 3.19 IEE100 pKa = 4.16 RR101 pKa = 11.84 VEE103 pKa = 3.83 IDD105 pKa = 3.27 GFEE108 pKa = 4.04 EE109 pKa = 5.24 GSFRR113 pKa = 11.84 LIPNARR119 pKa = 11.84 SGMSRR124 pKa = 11.84 GEE126 pKa = 3.88 INAYY130 pKa = 9.8 AALAEE135 pKa = 4.73 DD136 pKa = 5.71 LPDD139 pKa = 4.1 TLNHH143 pKa = 6.05 ATPFVDD149 pKa = 4.9 SEE151 pKa = 4.92 VEE153 pKa = 3.95 GTAWDD158 pKa = 4.18 EE159 pKa = 4.14 IEE161 pKa = 4.68 TFLDD165 pKa = 3.08 MCYY168 pKa = 10.57 SVLMQAWIVTCKK180 pKa = 10.52 CMTAPDD186 pKa = 3.62 QPAASIEE193 pKa = 3.98 KK194 pKa = 10.06 RR195 pKa = 11.84 LQKK198 pKa = 10.3 YY199 pKa = 8.17 RR200 pKa = 11.84 QQGRR204 pKa = 11.84 INPRR208 pKa = 11.84 YY209 pKa = 9.48 LLQPEE214 pKa = 4.17 ARR216 pKa = 11.84 RR217 pKa = 11.84 IIQNVIRR224 pKa = 11.84 KK225 pKa = 9.26 GMVVRR230 pKa = 11.84 HH231 pKa = 5.88 FLTFEE236 pKa = 4.09 LQLARR241 pKa = 11.84 AQSLVSNRR249 pKa = 11.84 YY250 pKa = 7.77 YY251 pKa = 11.74 AMVGDD256 pKa = 3.57 VGKK259 pKa = 10.91 YY260 pKa = 9.56 IEE262 pKa = 4.32 NCGMGGFFLTLKK274 pKa = 10.07 YY275 pKa = 10.78 ALGTRR280 pKa = 11.84 WPTLALAAFSGEE292 pKa = 4.03 LTKK295 pKa = 10.94 LKK297 pKa = 10.92 SLMALYY303 pKa = 8.09 QTLGEE308 pKa = 4.1 QARR311 pKa = 11.84 YY312 pKa = 8.72 LALLEE317 pKa = 4.94 SPHH320 pKa = 8.16 LMDD323 pKa = 5.63 FAAANYY329 pKa = 7.83 PLLYY333 pKa = 9.88 SYY335 pKa = 11.71 AMGIGYY341 pKa = 9.82 VLDD344 pKa = 3.66 VNMRR348 pKa = 11.84 NYY350 pKa = 10.82 AFSRR354 pKa = 11.84 SYY356 pKa = 10.23 MNKK359 pKa = 9.14 TYY361 pKa = 10.16 FQLGMEE367 pKa = 4.83 TARR370 pKa = 11.84 KK371 pKa = 7.78 QQGAVDD377 pKa = 3.49 MRR379 pKa = 11.84 MAEE382 pKa = 4.4 DD383 pKa = 3.8 LGLTQAEE390 pKa = 4.23 RR391 pKa = 11.84 TEE393 pKa = 4.24 MANTLAKK400 pKa = 9.63 LTTANRR406 pKa = 11.84 GADD409 pKa = 3.22 TRR411 pKa = 11.84 GGVNPFSSVTGTTQVPAAATGDD433 pKa = 3.78 TLEE436 pKa = 5.22 SYY438 pKa = 9.57 MAADD442 pKa = 4.53 RR443 pKa = 11.84 LRR445 pKa = 11.84 QRR447 pKa = 11.84 YY448 pKa = 9.11 ADD450 pKa = 4.45 AGTHH454 pKa = 6.39 DD455 pKa = 5.64 DD456 pKa = 4.11 EE457 pKa = 4.72 MPPLEE462 pKa = 4.75 EE463 pKa = 4.59 EE464 pKa = 4.57 EE465 pKa = 4.82 EE466 pKa = 4.78 DD467 pKa = 3.84 DD468 pKa = 3.74 TSAGPRR474 pKa = 11.84 TGPTLEE480 pKa = 4.1 QVALDD485 pKa = 3.73 IQNAAVGAPIHH496 pKa = 6.48 TDD498 pKa = 3.42 DD499 pKa = 5.38 LNAALGDD506 pKa = 3.89 LDD508 pKa = 3.57 II509 pKa = 6.39
Molecular weight: 56.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.952
IPC2_protein 5.003
IPC_protein 4.94
Toseland 4.838
ProMoST 5.067
Dawson 4.914
Bjellqvist 5.041
Wikipedia 4.774
Rodwell 4.813
Grimsley 4.762
Solomon 4.902
Lehninger 4.863
Nozaki 5.016
DTASelect 5.169
Thurlkill 4.838
EMBOSS 4.8
Sillero 5.092
Patrickios 3.973
IPC_peptide 4.914
IPC2_peptide 5.092
IPC2.peptide.svr19 5.043
Protein with the highest isoelectric point:
>sp|Q88434|L_PIV5 RNA-directed RNA polymerase L OS=Parainfluenza virus 5 (strain W3) OX=11208 GN=L PE=1 SV=1
MM1 pKa = 7.68 PSISIPADD9 pKa = 3.13 PTNPRR14 pKa = 11.84 QSIKK18 pKa = 10.76 AFPIVINSDD27 pKa = 3.08 GGEE30 pKa = 3.91 KK31 pKa = 10.56 GRR33 pKa = 11.84 LVKK36 pKa = 9.9 QLRR39 pKa = 11.84 TTYY42 pKa = 11.12 LNDD45 pKa = 4.07 LDD47 pKa = 3.93 THH49 pKa = 6.37 EE50 pKa = 4.97 PLVTFINTYY59 pKa = 10.24 GFIYY63 pKa = 10.18 EE64 pKa = 3.99 QDD66 pKa = 3.28 RR67 pKa = 11.84 GNTIVGEE74 pKa = 4.08 DD75 pKa = 3.34 QLGKK79 pKa = 10.38 KK80 pKa = 9.83 RR81 pKa = 11.84 EE82 pKa = 4.16 AVTAAMVTLGCGPNLPSLGNVLGQLRR108 pKa = 11.84 EE109 pKa = 4.0 FQVTVRR115 pKa = 11.84 KK116 pKa = 7.95 TSSKK120 pKa = 9.45 AEE122 pKa = 3.74 EE123 pKa = 4.02 MVFEE127 pKa = 4.11 IVKK130 pKa = 9.73 YY131 pKa = 8.79 PRR133 pKa = 11.84 IFRR136 pKa = 11.84 GHH138 pKa = 5.43 TLIQKK143 pKa = 10.21 GLVCVSAEE151 pKa = 4.2 KK152 pKa = 10.22 FVKK155 pKa = 10.57 SPGKK159 pKa = 9.4 IQSGMDD165 pKa = 3.34 YY166 pKa = 11.12 LFIPTFLSVTYY177 pKa = 9.89 CPAAIKK183 pKa = 9.84 FQVPGPMLKK192 pKa = 8.94 MRR194 pKa = 11.84 SRR196 pKa = 11.84 YY197 pKa = 6.47 TQSLQLEE204 pKa = 4.25 LMIRR208 pKa = 11.84 ILCKK212 pKa = 9.79 PDD214 pKa = 3.31 SPLMKK219 pKa = 10.14 VHH221 pKa = 6.4 TPDD224 pKa = 3.67 KK225 pKa = 10.25 EE226 pKa = 4.28 GRR228 pKa = 11.84 GCLVSVWLHH237 pKa = 4.57 VCNIFKK243 pKa = 10.67 SGNKK247 pKa = 9.49 NGSEE251 pKa = 3.74 WQEE254 pKa = 3.32 YY255 pKa = 7.21 WMRR258 pKa = 11.84 KK259 pKa = 7.19 CANMQLEE266 pKa = 4.3 VSIADD271 pKa = 3.19 MWGPTIIIHH280 pKa = 6.41 ARR282 pKa = 11.84 GHH284 pKa = 5.71 IPKK287 pKa = 9.82 SAKK290 pKa = 10.39 LFFGKK295 pKa = 10.44 GGWSCHH301 pKa = 5.65 PLHH304 pKa = 6.51 EE305 pKa = 4.59 VVPSVTKK312 pKa = 8.79 TLWSVGCEE320 pKa = 3.51 ITKK323 pKa = 10.6 AKK325 pKa = 10.62 AIIQEE330 pKa = 4.25 SSISLLVEE338 pKa = 3.81 TTDD341 pKa = 3.97 IISPKK346 pKa = 10.19 VKK348 pKa = 9.79 ISSKK352 pKa = 9.77 HH353 pKa = 4.19 RR354 pKa = 11.84 RR355 pKa = 11.84 FVKK358 pKa = 10.78 SNWGLFKK365 pKa = 10.08 KK366 pKa = 9.01 TKK368 pKa = 10.1 SLPNLTEE375 pKa = 4.03 LEE377 pKa = 4.23
Molecular weight: 42.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.923
IPC2_protein 8.785
IPC_protein 8.653
Toseland 9.604
ProMoST 9.253
Dawson 9.809
Bjellqvist 9.516
Wikipedia 9.911
Rodwell 10.306
Grimsley 9.838
Solomon 9.823
Lehninger 9.809
Nozaki 9.765
DTASelect 9.443
Thurlkill 9.677
EMBOSS 10.014
Sillero 9.765
Patrickios 9.502
IPC_peptide 9.838
IPC2_peptide 8.156
IPC2.peptide.svr19 7.738
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
4893
44
2255
611.6
68.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.56 ± 0.995
1.962 ± 0.273
4.66 ± 0.485
4.925 ± 0.655
3.638 ± 0.357
5.702 ± 0.581
1.737 ± 0.392
7.766 ± 0.706
4.558 ± 0.908
11.179 ± 0.767
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.452 ± 0.265
5.436 ± 0.477
5.109 ± 0.459
4.312 ± 0.493
4.68 ± 0.477
7.93 ± 0.533
7.46 ± 0.68
5.518 ± 0.731
1.165 ± 0.268
3.25 ± 0.497
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here